Experiment: MoLS4 without Biotin with Avidin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1847 and DVU1848 are separated by 162 nucleotides DVU1848 and pcm are separated by 3 nucleotides pcm and DVU1850 are separated by 224 nucleotides DVU1850 and DVU1851 are separated by 80 nucleotides
DVU1847: DVU1847 - conserved hypothetical protein (TIGR), at 1,915,787 to 1,916,680
DVU1847
DVU1848: DVU1848 - conserved hypothetical protein (TIGR), at 1,916,843 to 1,917,436
DVU1848
DVU1849: pcm - protein-L-isoaspartate O-methyltransferase (TIGR), at 1,917,440 to 1,918,072
pcm
DVU1850: DVU1850 - CBS domain protein (TIGR), at 1,918,297 to 1,918,725
DVU1850
DVU1851: DVU1851 - peptidase, M23/M37 family (TIGR), at 1,918,806 to 1,920,128
DVU1851
Position (kb)
1917
1918
1919 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1916.770 kb on + strand at 1916.778 kb on - strand at 1916.788 kb on - strand at 1916.929 kb on + strand, within DVU1848 at 1916.946 kb on + strand, within DVU1848 at 1916.959 kb on - strand, within DVU1848 at 1917.027 kb on + strand, within DVU1848 at 1917.075 kb on + strand, within DVU1848 at 1917.208 kb on + strand, within DVU1848 at 1917.251 kb on + strand, within DVU1848 at 1917.254 kb on - strand, within DVU1848 at 1917.347 kb on + strand, within DVU1848 at 1917.424 kb on + strand at 1917.571 kb on - strand, within pcm at 1917.665 kb on - strand, within pcm at 1917.693 kb on + strand, within pcm at 1917.775 kb on + strand, within pcm at 1917.812 kb on + strand, within pcm at 1917.812 kb on + strand, within pcm at 1917.897 kb on + strand, within pcm at 1917.944 kb on - strand, within pcm at 1918.224 kb on - strand at 1918.260 kb on + strand at 1918.457 kb on - strand, within DVU1850 at 1918.584 kb on + strand, within DVU1850 at 1918.709 kb on - strand at 1918.709 kb on - strand at 1918.727 kb on + strand at 1918.802 kb on + strand at 1918.911 kb on - strand at 1919.037 kb on - strand, within DVU1851
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Biotin with Avidin remove 1,916,770 + -0.3 1,916,778 - -0.7 1,916,788 - +1.0 1,916,929 + DVU1848 0.14 -0.3 1,916,946 + DVU1848 0.17 -0.1 1,916,959 - DVU1848 0.20 -0.6 1,917,027 + DVU1848 0.31 +2.3 1,917,075 + DVU1848 0.39 -1.1 1,917,208 + DVU1848 0.61 +0.2 1,917,251 + DVU1848 0.69 +0.2 1,917,254 - DVU1848 0.69 -0.1 1,917,347 + DVU1848 0.85 -0.2 1,917,424 + +0.1 1,917,571 - pcm DVU1849 0.21 -1.2 1,917,665 - pcm DVU1849 0.36 -0.4 1,917,693 + pcm DVU1849 0.40 -2.0 1,917,775 + pcm DVU1849 0.53 -0.5 1,917,812 + pcm DVU1849 0.59 -1.0 1,917,812 + pcm DVU1849 0.59 -1.3 1,917,897 + pcm DVU1849 0.72 +0.9 1,917,944 - pcm DVU1849 0.80 +0.3 1,918,224 - +0.2 1,918,260 + +0.2 1,918,457 - DVU1850 0.37 +0.0 1,918,584 + DVU1850 0.67 -1.5 1,918,709 - -0.0 1,918,709 - +0.6 1,918,727 + +0.6 1,918,802 + +0.8 1,918,911 - +0.1 1,919,037 - DVU1851 0.17 -0.8
Or see this region's nucleotide sequence