Experiment: MoLS4 without Biotin with Avidin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabF and DORF42491 overlap by 1 nucleotides DORF42491 and bioF overlap by 28 nucleotides bioF and bioD overlap by 19 nucleotides bioD and lysA-2 are separated by 155 nucleotides
DVU2563: fabF - beta-ketoacyl synthase, putative (TIGR), at 2,674,645 to 2,676,093
fabF
DORF42491: DORF42491 - SEED: FIG00602458: hypothetical protein, at 2,676,093 to 2,676,686
DORF42491
DVU2564: bioF - 8-amino-7-oxononanoate synthase (Dmitry Rodionov), at 2,676,659 to 2,677,933
bioF
DVU2565: bioD - dethiobiotin synthetase (TIGR), at 2,677,915 to 2,678,655
bioD
DVU2566: lysA-2 - diaminopimelate decarboxylase (TIGR), at 2,678,811 to 2,680,076
lysA-2
Position (kb)
2676
2677
2678 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2675.715 kb on - strand, within fabF at 2675.758 kb on - strand, within fabF at 2675.758 kb on - strand, within fabF at 2675.758 kb on - strand, within fabF at 2675.849 kb on - strand, within fabF at 2675.860 kb on + strand, within fabF at 2675.885 kb on - strand, within fabF at 2675.885 kb on - strand, within fabF at 2676.008 kb on + strand at 2676.016 kb on - strand at 2676.016 kb on - strand at 2676.016 kb on - strand at 2676.175 kb on - strand, within DORF42491 at 2676.264 kb on - strand, within DORF42491 at 2676.269 kb on - strand, within DORF42491 at 2676.284 kb on - strand, within DORF42491 at 2676.309 kb on + strand, within DORF42491 at 2676.326 kb on + strand, within DORF42491 at 2676.398 kb on + strand, within DORF42491 at 2676.446 kb on + strand, within DORF42491 at 2676.481 kb on + strand, within DORF42491 at 2676.510 kb on - strand, within DORF42491 at 2676.513 kb on - strand, within DORF42491 at 2676.515 kb on - strand, within DORF42491 at 2676.520 kb on - strand, within DORF42491 at 2676.543 kb on + strand, within DORF42491 at 2676.544 kb on + strand, within DORF42491 at 2676.554 kb on - strand, within DORF42491 at 2676.565 kb on + strand, within DORF42491 at 2676.565 kb on + strand, within DORF42491 at 2676.565 kb on + strand, within DORF42491 at 2676.567 kb on - strand, within DORF42491 at 2676.573 kb on - strand, within DORF42491 at 2676.641 kb on + strand at 2676.643 kb on + strand at 2676.651 kb on - strand at 2676.670 kb on - strand at 2676.671 kb on + strand at 2676.679 kb on - strand at 2676.681 kb on + strand at 2676.689 kb on - strand at 2676.689 kb on - strand at 2676.724 kb on - strand at 2676.766 kb on - strand at 2676.870 kb on + strand, within bioF at 2676.887 kb on + strand, within bioF at 2676.982 kb on - strand, within bioF at 2677.056 kb on - strand, within bioF at 2677.092 kb on - strand, within bioF at 2677.148 kb on - strand, within bioF at 2677.159 kb on - strand, within bioF at 2677.217 kb on - strand, within bioF at 2677.308 kb on - strand, within bioF at 2677.400 kb on + strand, within bioF at 2677.409 kb on - strand, within bioF at 2677.480 kb on + strand, within bioF at 2677.480 kb on + strand, within bioF at 2677.488 kb on - strand, within bioF at 2677.676 kb on - strand, within bioF at 2677.688 kb on - strand, within bioF at 2677.722 kb on + strand, within bioF at 2677.722 kb on + strand, within bioF at 2677.722 kb on + strand, within bioF at 2677.729 kb on - strand, within bioF at 2677.730 kb on - strand, within bioF at 2677.753 kb on - strand, within bioF at 2677.796 kb on + strand, within bioF at 2677.957 kb on + strand at 2678.031 kb on + strand, within bioD at 2678.031 kb on + strand, within bioD at 2678.036 kb on + strand, within bioD at 2678.039 kb on - strand, within bioD at 2678.052 kb on + strand, within bioD at 2678.060 kb on - strand, within bioD at 2678.060 kb on - strand, within bioD at 2678.266 kb on - strand, within bioD at 2678.288 kb on - strand, within bioD at 2678.298 kb on - strand, within bioD at 2678.365 kb on + strand, within bioD at 2678.373 kb on + strand, within bioD at 2678.403 kb on - strand, within bioD at 2678.474 kb on - strand, within bioD at 2678.493 kb on + strand, within bioD at 2678.508 kb on - strand, within bioD at 2678.549 kb on + strand, within bioD at 2678.616 kb on - strand at 2678.659 kb on + strand at 2678.659 kb on + strand at 2678.660 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.693 kb on - strand at 2678.724 kb on + strand at 2678.757 kb on + strand at 2678.794 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Biotin with Avidin remove 2,675,715 - fabF DVU2563 0.74 -3.6 2,675,758 - fabF DVU2563 0.77 -2.4 2,675,758 - fabF DVU2563 0.77 -2.3 2,675,758 - fabF DVU2563 0.77 -3.6 2,675,849 - fabF DVU2563 0.83 -2.5 2,675,860 + fabF DVU2563 0.84 -2.5 2,675,885 - fabF DVU2563 0.86 -2.0 2,675,885 - fabF DVU2563 0.86 -2.9 2,676,008 + -3.8 2,676,016 - -2.4 2,676,016 - -2.2 2,676,016 - -1.5 2,676,175 - DORF42491 0.14 -2.2 2,676,264 - DORF42491 0.29 -2.0 2,676,269 - DORF42491 0.30 -3.5 2,676,284 - DORF42491 0.32 -1.9 2,676,309 + DORF42491 0.36 -2.3 2,676,326 + DORF42491 0.39 -1.2 2,676,398 + DORF42491 0.51 -3.5 2,676,446 + DORF42491 0.59 -1.8 2,676,481 + DORF42491 0.65 -1.8 2,676,510 - DORF42491 0.70 -2.2 2,676,513 - DORF42491 0.71 -2.1 2,676,515 - DORF42491 0.71 -0.7 2,676,520 - DORF42491 0.72 -1.2 2,676,543 + DORF42491 0.76 -0.7 2,676,544 + DORF42491 0.76 -1.0 2,676,554 - DORF42491 0.78 -2.2 2,676,565 + DORF42491 0.79 -2.4 2,676,565 + DORF42491 0.79 -2.0 2,676,565 + DORF42491 0.79 -1.9 2,676,567 - DORF42491 0.80 -1.6 2,676,573 - DORF42491 0.81 -2.9 2,676,641 + -1.7 2,676,643 + +0.2 2,676,651 - -2.2 2,676,670 - -1.8 2,676,671 + -1.9 2,676,679 - -1.9 2,676,681 + -0.8 2,676,689 - -2.4 2,676,689 - -2.4 2,676,724 - -3.1 2,676,766 - -1.6 2,676,870 + bioF DVU2564 0.17 -2.6 2,676,887 + bioF DVU2564 0.18 -3.2 2,676,982 - bioF DVU2564 0.25 -1.6 2,677,056 - bioF DVU2564 0.31 -1.5 2,677,092 - bioF DVU2564 0.34 -3.7 2,677,148 - bioF DVU2564 0.38 -0.9 2,677,159 - bioF DVU2564 0.39 -1.9 2,677,217 - bioF DVU2564 0.44 -2.5 2,677,308 - bioF DVU2564 0.51 -2.3 2,677,400 + bioF DVU2564 0.58 -1.9 2,677,409 - bioF DVU2564 0.59 -3.0 2,677,480 + bioF DVU2564 0.64 -2.6 2,677,480 + bioF DVU2564 0.64 -2.1 2,677,488 - bioF DVU2564 0.65 -2.2 2,677,676 - bioF DVU2564 0.80 -2.5 2,677,688 - bioF DVU2564 0.81 -2.0 2,677,722 + bioF DVU2564 0.83 -2.2 2,677,722 + bioF DVU2564 0.83 -1.6 2,677,722 + bioF DVU2564 0.83 -1.1 2,677,729 - bioF DVU2564 0.84 -2.3 2,677,730 - bioF DVU2564 0.84 -2.5 2,677,753 - bioF DVU2564 0.86 -2.6 2,677,796 + bioF DVU2564 0.89 -1.9 2,677,957 + -2.8 2,678,031 + bioD DVU2565 0.16 -2.6 2,678,031 + bioD DVU2565 0.16 -2.3 2,678,036 + bioD DVU2565 0.16 -0.4 2,678,039 - bioD DVU2565 0.17 -3.2 2,678,052 + bioD DVU2565 0.18 -4.7 2,678,060 - bioD DVU2565 0.20 -1.9 2,678,060 - bioD DVU2565 0.20 -1.2 2,678,266 - bioD DVU2565 0.47 -0.6 2,678,288 - bioD DVU2565 0.50 -0.4 2,678,298 - bioD DVU2565 0.52 -2.7 2,678,365 + bioD DVU2565 0.61 -3.0 2,678,373 + bioD DVU2565 0.62 -1.6 2,678,403 - bioD DVU2565 0.66 -2.5 2,678,474 - bioD DVU2565 0.75 -1.7 2,678,493 + bioD DVU2565 0.78 -1.4 2,678,508 - bioD DVU2565 0.80 -2.3 2,678,549 + bioD DVU2565 0.86 -0.6 2,678,616 - -1.8 2,678,659 + +0.0 2,678,659 + +0.1 2,678,660 - -0.8 2,678,667 - -0.0 2,678,667 - -1.9 2,678,667 - +0.1 2,678,667 - -0.9 2,678,693 - -0.4 2,678,724 + +0.7 2,678,757 + +0.3 2,678,794 + -0.6
Or see this region's nucleotide sequence