Experiment: MoLS4 without Biotin with Avidin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioB and bioA overlap by 4 nucleotides bioA and DVU2560 overlap by 4 nucleotides DVU2560 and DVU2561 overlap by 4 nucleotides
DVU2558: bioB - biotin synthase (Dmitry Rodionov), at 2,669,895 to 2,670,827
bioB
DVU2559: bioA - adenosylmethionine--8-amino-7-oxononanoate aminotransferase (TIGR), at 2,670,824 to 2,672,452
bioA
DVU2560: DVU2560 - conserved domain protein (TIGR), at 2,672,449 to 2,672,835
DVU2560
DVU2561: DVU2561 - oxidoreductase, short chain dehydrogenase/reductase family (TIGR), at 2,672,832 to 2,673,860
DVU2561
Position (kb)
2670
2671
2672
2673 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2669.966 kb on + strand at 2669.974 kb on + strand at 2670.002 kb on - strand, within bioB at 2670.029 kb on + strand, within bioB at 2670.103 kb on + strand, within bioB at 2670.153 kb on + strand, within bioB at 2670.153 kb on - strand, within bioB at 2670.304 kb on - strand, within bioB at 2670.391 kb on + strand, within bioB at 2670.399 kb on - strand, within bioB at 2670.401 kb on + strand, within bioB at 2670.404 kb on - strand, within bioB at 2670.409 kb on - strand, within bioB at 2670.450 kb on - strand, within bioB at 2670.483 kb on + strand, within bioB at 2670.646 kb on - strand, within bioB at 2670.702 kb on - strand, within bioB at 2670.718 kb on - strand, within bioB at 2670.739 kb on + strand at 2670.776 kb on + strand at 2670.839 kb on - strand at 2670.858 kb on - strand at 2670.996 kb on - strand, within bioA at 2671.126 kb on + strand, within bioA at 2671.162 kb on + strand, within bioA at 2671.231 kb on - strand, within bioA at 2671.243 kb on - strand, within bioA at 2671.243 kb on - strand, within bioA at 2671.318 kb on - strand, within bioA at 2671.323 kb on - strand, within bioA at 2671.323 kb on - strand, within bioA at 2671.411 kb on - strand, within bioA at 2671.411 kb on - strand, within bioA at 2671.421 kb on + strand, within bioA at 2671.470 kb on + strand, within bioA at 2671.470 kb on + strand, within bioA at 2671.478 kb on - strand, within bioA at 2671.502 kb on + strand, within bioA at 2671.508 kb on - strand, within bioA at 2671.815 kb on - strand, within bioA at 2671.838 kb on + strand, within bioA at 2671.939 kb on + strand, within bioA at 2671.951 kb on + strand, within bioA at 2671.961 kb on - strand, within bioA at 2671.967 kb on + strand, within bioA at 2671.967 kb on + strand, within bioA at 2671.969 kb on - strand, within bioA at 2671.975 kb on - strand, within bioA at 2671.995 kb on + strand, within bioA at 2672.071 kb on - strand, within bioA at 2672.117 kb on - strand, within bioA at 2672.127 kb on + strand, within bioA at 2672.127 kb on + strand, within bioA at 2672.140 kb on - strand, within bioA at 2672.160 kb on + strand, within bioA at 2672.285 kb on + strand, within bioA at 2672.285 kb on + strand, within bioA at 2672.293 kb on - strand at 2672.396 kb on - strand at 2672.422 kb on - strand at 2672.456 kb on - strand at 2672.509 kb on - strand, within DVU2560 at 2672.519 kb on - strand, within DVU2560 at 2672.576 kb on - strand, within DVU2560 at 2672.662 kb on + strand, within DVU2560 at 2672.672 kb on + strand, within DVU2560 at 2672.674 kb on - strand, within DVU2560 at 2672.684 kb on + strand, within DVU2560 at 2672.695 kb on - strand, within DVU2560 at 2672.702 kb on - strand, within DVU2560 at 2672.728 kb on + strand, within DVU2560 at 2672.830 kb on + strand at 2672.853 kb on + strand at 2672.904 kb on + strand at 2672.906 kb on + strand at 2672.914 kb on - strand at 2672.914 kb on - strand at 2672.931 kb on - strand at 2672.953 kb on + strand, within DVU2561 at 2673.041 kb on - strand, within DVU2561 at 2673.077 kb on - strand, within DVU2561 at 2673.106 kb on + strand, within DVU2561 at 2673.114 kb on - strand, within DVU2561 at 2673.124 kb on - strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.145 kb on - strand, within DVU2561 at 2673.153 kb on + strand, within DVU2561 at 2673.165 kb on + strand, within DVU2561 at 2673.197 kb on - strand, within DVU2561 at 2673.301 kb on - strand, within DVU2561
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Biotin with Avidin remove 2,669,966 + -2.4 2,669,974 + -1.6 2,670,002 - bioB DVU2558 0.11 -2.8 2,670,029 + bioB DVU2558 0.14 -1.7 2,670,103 + bioB DVU2558 0.22 -1.4 2,670,153 + bioB DVU2558 0.28 -2.1 2,670,153 - bioB DVU2558 0.28 -1.9 2,670,304 - bioB DVU2558 0.44 -1.9 2,670,391 + bioB DVU2558 0.53 -3.5 2,670,399 - bioB DVU2558 0.54 -2.3 2,670,401 + bioB DVU2558 0.54 -2.0 2,670,404 - bioB DVU2558 0.55 -1.2 2,670,409 - bioB DVU2558 0.55 -3.0 2,670,450 - bioB DVU2558 0.59 -0.7 2,670,483 + bioB DVU2558 0.63 -0.4 2,670,646 - bioB DVU2558 0.80 -1.5 2,670,702 - bioB DVU2558 0.86 -2.7 2,670,718 - bioB DVU2558 0.88 -2.4 2,670,739 + -0.9 2,670,776 + -2.1 2,670,839 - -1.3 2,670,858 - -2.8 2,670,996 - bioA DVU2559 0.11 -0.7 2,671,126 + bioA DVU2559 0.19 -2.0 2,671,162 + bioA DVU2559 0.21 -1.6 2,671,231 - bioA DVU2559 0.25 -2.9 2,671,243 - bioA DVU2559 0.26 -3.0 2,671,243 - bioA DVU2559 0.26 -1.8 2,671,318 - bioA DVU2559 0.30 -3.9 2,671,323 - bioA DVU2559 0.31 -3.0 2,671,323 - bioA DVU2559 0.31 -1.5 2,671,411 - bioA DVU2559 0.36 -2.5 2,671,411 - bioA DVU2559 0.36 -1.0 2,671,421 + bioA DVU2559 0.37 -2.4 2,671,470 + bioA DVU2559 0.40 -1.8 2,671,470 + bioA DVU2559 0.40 -2.1 2,671,478 - bioA DVU2559 0.40 -0.5 2,671,502 + bioA DVU2559 0.42 -1.3 2,671,508 - bioA DVU2559 0.42 -2.3 2,671,815 - bioA DVU2559 0.61 -1.5 2,671,838 + bioA DVU2559 0.62 -2.0 2,671,939 + bioA DVU2559 0.68 -1.7 2,671,951 + bioA DVU2559 0.69 -2.7 2,671,961 - bioA DVU2559 0.70 -3.1 2,671,967 + bioA DVU2559 0.70 -2.0 2,671,967 + bioA DVU2559 0.70 -2.3 2,671,969 - bioA DVU2559 0.70 -2.4 2,671,975 - bioA DVU2559 0.71 -1.7 2,671,995 + bioA DVU2559 0.72 -2.3 2,672,071 - bioA DVU2559 0.77 -2.2 2,672,117 - bioA DVU2559 0.79 -4.9 2,672,127 + bioA DVU2559 0.80 -0.6 2,672,127 + bioA DVU2559 0.80 -0.7 2,672,140 - bioA DVU2559 0.81 -2.5 2,672,160 + bioA DVU2559 0.82 -2.5 2,672,285 + bioA DVU2559 0.90 -1.9 2,672,285 + bioA DVU2559 0.90 -3.1 2,672,293 - -1.8 2,672,396 - -2.2 2,672,422 - -1.2 2,672,456 - -0.1 2,672,509 - DVU2560 0.16 -2.2 2,672,519 - DVU2560 0.18 -2.3 2,672,576 - DVU2560 0.33 -2.5 2,672,662 + DVU2560 0.55 -2.5 2,672,672 + DVU2560 0.58 -1.8 2,672,674 - DVU2560 0.58 -2.2 2,672,684 + DVU2560 0.61 -2.4 2,672,695 - DVU2560 0.64 -1.8 2,672,702 - DVU2560 0.65 -2.3 2,672,728 + DVU2560 0.72 -0.7 2,672,830 + -0.3 2,672,853 + -2.3 2,672,904 + -1.3 2,672,906 + -0.7 2,672,914 - -0.3 2,672,914 - -2.2 2,672,931 - -2.1 2,672,953 + DVU2561 0.12 -2.7 2,673,041 - DVU2561 0.20 -1.6 2,673,077 - DVU2561 0.24 -0.4 2,673,106 + DVU2561 0.27 -2.3 2,673,114 - DVU2561 0.27 -3.5 2,673,124 - DVU2561 0.28 -2.3 2,673,127 + DVU2561 0.29 -1.8 2,673,127 + DVU2561 0.29 -2.3 2,673,127 + DVU2561 0.29 -1.9 2,673,127 + DVU2561 0.29 -3.4 2,673,135 - DVU2561 0.29 -2.4 2,673,135 - DVU2561 0.29 -2.8 2,673,135 - DVU2561 0.29 -1.9 2,673,135 - DVU2561 0.29 -2.2 2,673,145 - DVU2561 0.30 -3.1 2,673,153 + DVU2561 0.31 -2.8 2,673,165 + DVU2561 0.32 -1.6 2,673,197 - DVU2561 0.35 -2.2 2,673,301 - DVU2561 0.46 -1.5
Or see this region's nucleotide sequence