Experiment: Survival; Treatment=FW300; TreatmentDuration=2hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS01660 and ABCV34_RS01665 are separated by 23 nucleotides ABCV34_RS01665 and ABCV34_RS01670 are separated by 52 nucleotides
ABCV34_RS01660: ABCV34_RS01660 - bifunctional 2-methylcitrate dehydratase/aconitate hydratase, at 356,525 to 357,976
_RS01660
ABCV34_RS01665: ABCV34_RS01665 - 2-methylcitrate synthase, at 358,000 to 359,265
_RS01665
ABCV34_RS01670: ABCV34_RS01670 - methylisocitrate lyase, at 359,318 to 360,217
_RS01670
Position (kb) 357
358
359
360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 357.150 kb on + strand, within ABCV34_RS01660 at 357.151 kb on - strand, within ABCV34_RS01660 at 357.151 kb on - strand, within ABCV34_RS01660 at 357.900 kb on + strand at 357.913 kb on - strand at 357.965 kb on - strand at 357.965 kb on - strand at 358.001 kb on + strand at 358.001 kb on + strand at 358.001 kb on + strand at 358.001 kb on + strand at 358.002 kb on - strand at 358.002 kb on - strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.049 kb on + strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.050 kb on - strand at 358.176 kb on - strand, within ABCV34_RS01665 at 358.340 kb on + strand, within ABCV34_RS01665 at 358.341 kb on - strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.511 kb on + strand, within ABCV34_RS01665 at 358.512 kb on - strand, within ABCV34_RS01665 at 358.512 kb on - strand, within ABCV34_RS01665 at 358.512 kb on - strand, within ABCV34_RS01665 at 358.512 kb on - strand, within ABCV34_RS01665 at 358.512 kb on - strand, within ABCV34_RS01665 at 358.581 kb on - strand, within ABCV34_RS01665 at 358.712 kb on + strand, within ABCV34_RS01665 at 358.712 kb on + strand, within ABCV34_RS01665 at 358.718 kb on + strand, within ABCV34_RS01665 at 358.724 kb on + strand, within ABCV34_RS01665 at 358.725 kb on - strand, within ABCV34_RS01665 at 358.725 kb on - strand, within ABCV34_RS01665 at 358.934 kb on + strand, within ABCV34_RS01665 at 358.934 kb on + strand, within ABCV34_RS01665 at 358.934 kb on + strand, within ABCV34_RS01665 at 358.935 kb on - strand, within ABCV34_RS01665 at 358.935 kb on - strand, within ABCV34_RS01665 at 358.935 kb on - strand, within ABCV34_RS01665 at 358.935 kb on - strand, within ABCV34_RS01665 at 358.935 kb on - strand, within ABCV34_RS01665 at 359.394 kb on + strand at 359.394 kb on + strand at 359.395 kb on - strand at 359.395 kb on - strand at 359.463 kb on + strand, within ABCV34_RS01670 at 359.512 kb on - strand, within ABCV34_RS01670 at 359.600 kb on + strand, within ABCV34_RS01670 at 359.600 kb on + strand, within ABCV34_RS01670 at 359.600 kb on + strand, within ABCV34_RS01670 at 359.600 kb on + strand, within ABCV34_RS01670 at 359.601 kb on - strand, within ABCV34_RS01670 at 359.614 kb on + strand, within ABCV34_RS01670 at 359.614 kb on + strand, within ABCV34_RS01670 at 359.628 kb on + strand, within ABCV34_RS01670 at 359.628 kb on + strand, within ABCV34_RS01670 at 359.628 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.643 kb on + strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.644 kb on - strand, within ABCV34_RS01670 at 359.754 kb on + strand, within ABCV34_RS01670 at 359.861 kb on + strand, within ABCV34_RS01670 at 359.861 kb on + strand, within ABCV34_RS01670 at 360.023 kb on + strand, within ABCV34_RS01670 at 360.023 kb on + strand, within ABCV34_RS01670 at 360.023 kb on + strand, within ABCV34_RS01670 at 360.024 kb on - strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.087 kb on + strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670 at 360.088 kb on - strand, within ABCV34_RS01670
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300; TreatmentDuration=2hrs; Outgrowth=R2A_Kan10_Lactate remove 357,150 + ABCV34_RS01660 0.43 -1.2 357,151 - ABCV34_RS01660 0.43 +0.4 357,151 - ABCV34_RS01660 0.43 -0.2 357,900 + -0.8 357,913 - +0.8 357,965 - +0.8 357,965 - -0.6 358,001 + -1.6 358,001 + -1.8 358,001 + +0.1 358,001 + +1.4 358,002 - -0.6 358,002 - -0.2 358,049 + -1.5 358,049 + -1.8 358,049 + -3.3 358,049 + -1.0 358,049 + +0.0 358,049 + +0.5 358,049 + -1.3 358,049 + -1.7 358,049 + -2.9 358,049 + -1.1 358,050 - -2.2 358,050 - +0.4 358,050 - -1.3 358,050 - -1.4 358,050 - -3.0 358,050 - -0.3 358,050 - -1.3 358,050 - -0.0 358,050 - -1.1 358,050 - -0.8 358,176 - ABCV34_RS01665 0.14 -1.7 358,340 + ABCV34_RS01665 0.27 -2.7 358,341 - ABCV34_RS01665 0.27 -0.5 358,511 + ABCV34_RS01665 0.40 -1.3 358,511 + ABCV34_RS01665 0.40 -1.4 358,511 + ABCV34_RS01665 0.40 +0.4 358,511 + ABCV34_RS01665 0.40 +0.3 358,511 + ABCV34_RS01665 0.40 -1.3 358,511 + ABCV34_RS01665 0.40 -0.8 358,511 + ABCV34_RS01665 0.40 -1.6 358,511 + ABCV34_RS01665 0.40 +1.7 358,511 + ABCV34_RS01665 0.40 -2.3 358,511 + ABCV34_RS01665 0.40 -0.7 358,511 + ABCV34_RS01665 0.40 +1.5 358,512 - ABCV34_RS01665 0.40 -0.6 358,512 - ABCV34_RS01665 0.40 +1.4 358,512 - ABCV34_RS01665 0.40 -1.6 358,512 - ABCV34_RS01665 0.40 -2.2 358,512 - ABCV34_RS01665 0.40 -2.0 358,581 - ABCV34_RS01665 0.46 -0.8 358,712 + ABCV34_RS01665 0.56 -1.5 358,712 + ABCV34_RS01665 0.56 -1.3 358,718 + ABCV34_RS01665 0.57 -0.3 358,724 + ABCV34_RS01665 0.57 -1.0 358,725 - ABCV34_RS01665 0.57 -0.8 358,725 - ABCV34_RS01665 0.57 -1.2 358,934 + ABCV34_RS01665 0.74 -0.6 358,934 + ABCV34_RS01665 0.74 -2.3 358,934 + ABCV34_RS01665 0.74 -0.6 358,935 - ABCV34_RS01665 0.74 -0.3 358,935 - ABCV34_RS01665 0.74 -1.6 358,935 - ABCV34_RS01665 0.74 -0.8 358,935 - ABCV34_RS01665 0.74 -0.3 358,935 - ABCV34_RS01665 0.74 +1.5 359,394 + -1.7 359,394 + +0.7 359,395 - -0.3 359,395 - -2.5 359,463 + ABCV34_RS01670 0.16 +0.2 359,512 - ABCV34_RS01670 0.22 +3.1 359,600 + ABCV34_RS01670 0.31 -2.1 359,600 + ABCV34_RS01670 0.31 -2.0 359,600 + ABCV34_RS01670 0.31 -0.3 359,600 + ABCV34_RS01670 0.31 +0.4 359,601 - ABCV34_RS01670 0.31 -0.4 359,614 + ABCV34_RS01670 0.33 -0.2 359,614 + ABCV34_RS01670 0.33 -0.8 359,628 + ABCV34_RS01670 0.34 -1.5 359,628 + ABCV34_RS01670 0.34 -1.0 359,628 + ABCV34_RS01670 0.34 +0.1 359,643 + ABCV34_RS01670 0.36 -1.2 359,643 + ABCV34_RS01670 0.36 -1.8 359,643 + ABCV34_RS01670 0.36 -3.4 359,643 + ABCV34_RS01670 0.36 -2.7 359,643 + ABCV34_RS01670 0.36 -1.7 359,643 + ABCV34_RS01670 0.36 -3.2 359,643 + ABCV34_RS01670 0.36 +1.0 359,643 + ABCV34_RS01670 0.36 -2.9 359,644 - ABCV34_RS01670 0.36 -1.3 359,644 - ABCV34_RS01670 0.36 -0.9 359,644 - ABCV34_RS01670 0.36 -1.0 359,644 - ABCV34_RS01670 0.36 +0.8 359,644 - ABCV34_RS01670 0.36 +0.7 359,644 - ABCV34_RS01670 0.36 -0.3 359,754 + ABCV34_RS01670 0.48 -1.8 359,861 + ABCV34_RS01670 0.60 -0.8 359,861 + ABCV34_RS01670 0.60 -1.3 360,023 + ABCV34_RS01670 0.78 -0.6 360,023 + ABCV34_RS01670 0.78 +1.2 360,023 + ABCV34_RS01670 0.78 -1.2 360,024 - ABCV34_RS01670 0.78 +0.2 360,087 + ABCV34_RS01670 0.85 -1.7 360,087 + ABCV34_RS01670 0.85 +1.0 360,087 + ABCV34_RS01670 0.85 -2.3 360,087 + ABCV34_RS01670 0.85 -2.2 360,087 + ABCV34_RS01670 0.85 -1.2 360,087 + ABCV34_RS01670 0.85 -0.6 360,087 + ABCV34_RS01670 0.85 +0.3 360,088 - ABCV34_RS01670 0.86 -0.3 360,088 - ABCV34_RS01670 0.86 -1.3 360,088 - ABCV34_RS01670 0.86 -1.0 360,088 - ABCV34_RS01670 0.86 -1.8 360,088 - ABCV34_RS01670 0.86 -1.6 360,088 - ABCV34_RS01670 0.86 -1.3 360,088 - ABCV34_RS01670 0.86 -1.7
Or see this region's nucleotide sequence