Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03360 and MMP_RS03365 are separated by 19 nucleotides MMP_RS03365 and MMP_RS03370 are separated by 39 nucleotides MMP_RS03370 and MMP_RS03375 are separated by 147 nucleotides MMP_RS03375 and MMP_RS03380 are separated by 142 nucleotides
MMP_RS03360: MMP_RS03360 - 50S ribosomal protein L24, at 632,868 to 633,056
_RS03360
MMP_RS03365: MMP_RS03365 - 30S ribosomal protein S28e, at 633,076 to 633,306
_RS03365
MMP_RS03370: MMP_RS03370 - 50S ribosomal protein L7Ae, at 633,346 to 633,699
_RS03370
MMP_RS03375: MMP_RS03375 - methanogenesis marker 12 protein, at 633,847 to 634,824
_RS03375
MMP_RS03380: MMP_RS03380 - DUF354 domain-containing protein, at 634,967 to 635,980
_RS03380
Position (kb) 633
634
635 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 633.691 kb on - strand at 633.692 kb on + strand at 633.790 kb on - strand at 633.793 kb on + strand at 633.810 kb on + strand at 633.816 kb on - strand at 633.839 kb on - strand at 633.889 kb on - strand at 634.127 kb on - strand at 634.333 kb on - strand at 634.397 kb on - strand at 634.398 kb on + strand at 634.588 kb on - strand at 634.655 kb on + strand at 634.843 kb on - strand at 634.847 kb on + strand at 634.867 kb on - strand at 634.870 kb on + strand at 634.874 kb on + strand at 634.884 kb on - strand at 634.884 kb on - strand at 634.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE remove 633,691 - -1.2 633,692 + -2.7 633,790 - -3.2 633,793 + -3.2 633,810 + -3.3 633,816 - -2.6 633,839 - +0.2 633,889 - -2.0 634,127 - -3.3 634,333 - -3.3 634,397 - -1.3 634,398 + -4.0 634,588 - -0.9 634,655 + -2.5 634,843 - +0.5 634,847 + +1.4 634,867 - -1.2 634,870 + -2.3 634,874 + -1.6 634,884 - -3.6 634,884 - -4.3 634,974 - -2.2
Or see this region's nucleotide sequence