Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1048 and Ac3H11_938 are separated by 67 nucleotides Ac3H11_938 and Ac3H11_939 are separated by 125 nucleotides Ac3H11_939 and Ac3H11_940 are separated by 93 nucleotides
Ac3H11_1048 - tRNA-Leu-TAA, at 58,102 to 58,191
_1048
Ac3H11_938 - FIG00582348: hypothetical protein, at 58,259 to 58,843
_938
Ac3H11_939 - COG1451: Predicted metal-dependent hydrolase, at 58,969 to 59,508
_939
Ac3H11_940 - Glutamate racemase (EC 5.1.1.3), at 59,602 to 60,495
_940
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 58.196 kb on + strand at 58.217 kb on + strand at 58.217 kb on + strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.218 kb on - strand at 58.316 kb on + strand at 58.316 kb on + strand at 58.358 kb on + strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.359 kb on - strand, within Ac3H11_938 at 58.517 kb on + strand, within Ac3H11_938 at 58.517 kb on + strand, within Ac3H11_938 at 58.518 kb on - strand, within Ac3H11_938 at 58.518 kb on - strand, within Ac3H11_938 at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.934 kb on + strand at 58.935 kb on - strand at 58.935 kb on - strand at 58.935 kb on - strand at 58.935 kb on - strand at 59.002 kb on + strand at 59.003 kb on - strand at 59.003 kb on - strand at 59.014 kb on + strand at 59.089 kb on + strand, within Ac3H11_939 at 59.089 kb on + strand, within Ac3H11_939 at 59.089 kb on + strand, within Ac3H11_939 at 59.090 kb on - strand, within Ac3H11_939 at 59.128 kb on + strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.129 kb on - strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.134 kb on + strand, within Ac3H11_939 at 59.159 kb on - strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.392 kb on + strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.393 kb on - strand, within Ac3H11_939 at 59.422 kb on + strand, within Ac3H11_939 at 59.422 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.431 kb on + strand, within Ac3H11_939 at 59.432 kb on - strand, within Ac3H11_939 at 59.432 kb on - strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.452 kb on + strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.453 kb on - strand, within Ac3H11_939 at 59.535 kb on + strand at 59.536 kb on - strand at 59.542 kb on - strand at 59.557 kb on - strand at 59.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 58,196 + -2.6 58,217 + +1.4 58,217 + +0.7 58,218 - -1.3 58,218 - -0.8 58,218 - -0.6 58,218 - +0.7 58,316 + -0.3 58,316 + +0.9 58,358 + Ac3H11_938 0.17 -1.0 58,359 - Ac3H11_938 0.17 -2.0 58,359 - Ac3H11_938 0.17 +0.9 58,359 - Ac3H11_938 0.17 -1.8 58,517 + Ac3H11_938 0.44 -1.7 58,517 + Ac3H11_938 0.44 -1.5 58,518 - Ac3H11_938 0.44 +0.9 58,518 - Ac3H11_938 0.44 -2.3 58,934 + +0.3 58,934 + -0.3 58,934 + +4.4 58,934 + -0.3 58,934 + +0.9 58,935 - +0.6 58,935 - -0.2 58,935 - -0.6 58,935 - +0.4 59,002 + -0.2 59,003 - +0.8 59,003 - +0.7 59,014 + -0.3 59,089 + Ac3H11_939 0.22 +0.6 59,089 + Ac3H11_939 0.22 +1.9 59,089 + Ac3H11_939 0.22 -0.5 59,090 - Ac3H11_939 0.22 +4.2 59,128 + Ac3H11_939 0.29 -2.6 59,129 - Ac3H11_939 0.30 +2.4 59,129 - Ac3H11_939 0.30 -2.2 59,129 - Ac3H11_939 0.30 +0.5 59,129 - Ac3H11_939 0.30 +1.9 59,134 + Ac3H11_939 0.31 -0.4 59,134 + Ac3H11_939 0.31 -1.6 59,134 + Ac3H11_939 0.31 -0.7 59,159 - Ac3H11_939 0.35 +1.0 59,392 + Ac3H11_939 0.78 -2.2 59,392 + Ac3H11_939 0.78 +2.8 59,392 + Ac3H11_939 0.78 +1.7 59,392 + Ac3H11_939 0.78 -0.3 59,392 + Ac3H11_939 0.78 +0.9 59,392 + Ac3H11_939 0.78 +1.4 59,392 + Ac3H11_939 0.78 -1.1 59,393 - Ac3H11_939 0.79 -2.2 59,393 - Ac3H11_939 0.79 -0.7 59,393 - Ac3H11_939 0.79 +0.8 59,393 - Ac3H11_939 0.79 -0.7 59,393 - Ac3H11_939 0.79 -0.7 59,422 + Ac3H11_939 0.84 -2.0 59,422 + Ac3H11_939 0.84 +0.0 59,431 + Ac3H11_939 0.86 -1.5 59,431 + Ac3H11_939 0.86 -0.3 59,431 + Ac3H11_939 0.86 -1.2 59,432 - Ac3H11_939 0.86 -0.7 59,432 - Ac3H11_939 0.86 +2.0 59,452 + Ac3H11_939 0.89 -1.9 59,452 + Ac3H11_939 0.89 -0.3 59,452 + Ac3H11_939 0.89 +0.4 59,452 + Ac3H11_939 0.89 -0.5 59,453 - Ac3H11_939 0.90 -0.8 59,453 - Ac3H11_939 0.90 +2.5 59,453 - Ac3H11_939 0.90 +1.5 59,453 - Ac3H11_939 0.90 -0.0 59,535 + -0.4 59,536 - -1.4 59,542 - -0.3 59,557 - +0.5 59,557 - -1.8
Or see this region's nucleotide sequence