Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_71 and Ac3H11_72 are separated by 166 nucleotides Ac3H11_72 and Ac3H11_73 are separated by 215 nucleotides Ac3H11_73 and Ac3H11_74 are separated by 27 nucleotides
Ac3H11_71 - Extracellular ligand-binding receptor, at 75,001 to 76,149
_71
Ac3H11_72 - D-(-)-3-hydroxybutyrate oligomer hydrolase, at 76,316 to 77,191
_72
Ac3H11_73 - Phenylalanyl-tRNA synthetase beta subunit, at 77,407 to 77,874
_73
Ac3H11_74 - hypothetical protein, at 77,902 to 78,144
_74
Position (kb) 76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 75.394 kb on + strand, within Ac3H11_71 at 75.394 kb on + strand, within Ac3H11_71 at 75.394 kb on + strand, within Ac3H11_71 at 75.395 kb on - strand, within Ac3H11_71 at 75.395 kb on - strand, within Ac3H11_71 at 75.526 kb on + strand, within Ac3H11_71 at 75.527 kb on - strand, within Ac3H11_71 at 75.527 kb on - strand, within Ac3H11_71 at 75.760 kb on + strand, within Ac3H11_71 at 75.760 kb on + strand, within Ac3H11_71 at 75.761 kb on - strand, within Ac3H11_71 at 75.761 kb on - strand, within Ac3H11_71 at 75.931 kb on + strand, within Ac3H11_71 at 75.932 kb on - strand, within Ac3H11_71 at 76.045 kb on + strand at 76.046 kb on - strand at 76.046 kb on - strand at 76.073 kb on - strand at 76.073 kb on - strand at 76.103 kb on - strand at 76.205 kb on - strand at 76.361 kb on + strand at 76.373 kb on + strand at 76.373 kb on + strand at 76.547 kb on + strand, within Ac3H11_72 at 76.764 kb on - strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.965 kb on - strand, within Ac3H11_72 at 76.983 kb on - strand, within Ac3H11_72 at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.267 kb on + strand at 77.268 kb on - strand at 77.367 kb on + strand at 77.473 kb on + strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.678 kb on - strand, within Ac3H11_73 at 77.866 kb on + strand at 77.866 kb on + strand at 77.867 kb on - strand at 77.867 kb on - strand at 77.872 kb on + strand at 77.873 kb on - strand at 78.012 kb on - strand, within Ac3H11_74 at 78.012 kb on - strand, within Ac3H11_74 at 78.123 kb on - strand at 78.155 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 75,394 + Ac3H11_71 0.34 +0.9 75,394 + Ac3H11_71 0.34 +0.7 75,394 + Ac3H11_71 0.34 +1.1 75,395 - Ac3H11_71 0.34 -0.3 75,395 - Ac3H11_71 0.34 -2.9 75,526 + Ac3H11_71 0.46 +2.0 75,527 - Ac3H11_71 0.46 -2.5 75,527 - Ac3H11_71 0.46 +1.6 75,760 + Ac3H11_71 0.66 +3.6 75,760 + Ac3H11_71 0.66 -0.4 75,761 - Ac3H11_71 0.66 -1.0 75,761 - Ac3H11_71 0.66 -0.5 75,931 + Ac3H11_71 0.81 -0.1 75,932 - Ac3H11_71 0.81 -0.6 76,045 + -0.3 76,046 - -0.1 76,046 - +0.7 76,073 - -0.5 76,073 - -0.4 76,103 - -0.7 76,205 - -1.8 76,361 + -0.7 76,373 + -2.5 76,373 + -0.1 76,547 + Ac3H11_72 0.26 +2.7 76,764 - Ac3H11_72 0.51 +0.0 76,826 + Ac3H11_72 0.58 -0.2 76,826 + Ac3H11_72 0.58 -2.9 76,826 + Ac3H11_72 0.58 +2.7 76,826 + Ac3H11_72 0.58 -0.3 76,965 - Ac3H11_72 0.74 -3.2 76,983 - Ac3H11_72 0.76 -0.6 77,241 - +1.7 77,241 - -1.6 77,241 - -0.3 77,241 - +0.1 77,267 + -0.3 77,268 - +0.0 77,367 + -2.0 77,473 + Ac3H11_73 0.14 +0.2 77,474 - Ac3H11_73 0.14 +0.7 77,474 - Ac3H11_73 0.14 +0.4 77,474 - Ac3H11_73 0.14 +2.4 77,474 - Ac3H11_73 0.14 +0.6 77,668 + Ac3H11_73 0.56 +0.2 77,668 + Ac3H11_73 0.56 +0.6 77,678 - Ac3H11_73 0.58 +1.2 77,866 + +1.2 77,866 + -2.0 77,867 - -1.0 77,867 - +4.8 77,872 + -1.8 77,873 - +2.4 78,012 - Ac3H11_74 0.45 +0.5 78,012 - Ac3H11_74 0.45 +0.8 78,123 - +0.8 78,155 + +0.9
Or see this region's nucleotide sequence