Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_68 and Ac3H11_69 are separated by 16 nucleotides Ac3H11_69 and Ac3H11_70 are separated by 93 nucleotides
Ac3H11_68 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 70,564 to 71,550
_68
Ac3H11_69 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 71,567 to 72,640
_69
Ac3H11_70 - D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22), at 72,734 to 74,878
_70
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 70.826 kb on - strand, within Ac3H11_68 at 71.443 kb on + strand, within Ac3H11_68 at 71.444 kb on - strand, within Ac3H11_68 at 71.488 kb on - strand at 71.750 kb on + strand, within Ac3H11_69 at 71.751 kb on - strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.117 kb on - strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.254 kb on + strand, within Ac3H11_69 at 72.255 kb on - strand, within Ac3H11_69 at 72.329 kb on + strand, within Ac3H11_69 at 72.330 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.459 kb on - strand, within Ac3H11_69 at 72.578 kb on + strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.885 kb on - strand at 73.068 kb on - strand, within Ac3H11_70 at 73.260 kb on - strand, within Ac3H11_70 at 73.260 kb on - strand, within Ac3H11_70 at 73.314 kb on - strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.566 kb on - strand, within Ac3H11_70 at 73.566 kb on - strand, within Ac3H11_70
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 70,826 - Ac3H11_68 0.27 -0.9 71,443 + Ac3H11_68 0.89 +2.1 71,444 - Ac3H11_68 0.89 +0.4 71,488 - +0.7 71,750 + Ac3H11_69 0.17 -0.3 71,751 - Ac3H11_69 0.17 +0.3 72,116 + Ac3H11_69 0.51 +0.2 72,116 + Ac3H11_69 0.51 -0.1 72,117 - Ac3H11_69 0.51 +0.7 72,119 + Ac3H11_69 0.51 +2.0 72,119 + Ac3H11_69 0.51 -0.3 72,119 + Ac3H11_69 0.51 -0.1 72,254 + Ac3H11_69 0.64 +2.6 72,255 - Ac3H11_69 0.64 +0.7 72,329 + Ac3H11_69 0.71 -2.5 72,330 - Ac3H11_69 0.71 +1.5 72,414 - Ac3H11_69 0.79 -0.2 72,414 - Ac3H11_69 0.79 -0.5 72,459 - Ac3H11_69 0.83 -0.1 72,578 + -0.8 72,579 - -1.6 72,579 - -0.2 72,884 + -2.0 72,884 + -0.3 72,884 + -2.0 72,884 + -0.1 72,884 + -3.2 72,885 - +0.8 73,068 - Ac3H11_70 0.16 -1.4 73,260 - Ac3H11_70 0.25 -0.3 73,260 - Ac3H11_70 0.25 +0.3 73,314 - Ac3H11_70 0.27 +0.1 73,565 + Ac3H11_70 0.39 -0.5 73,565 + Ac3H11_70 0.39 -3.8 73,565 + Ac3H11_70 0.39 -1.1 73,565 + Ac3H11_70 0.39 -0.6 73,565 + Ac3H11_70 0.39 -0.2 73,565 + Ac3H11_70 0.39 +0.3 73,566 - Ac3H11_70 0.39 -2.2 73,566 - Ac3H11_70 0.39 -1.7
Or see this region's nucleotide sequence