Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_5002 and Ac3H11_5003 overlap by 4 nucleotides Ac3H11_5003 and Ac3H11_5004 are separated by 37 nucleotides Ac3H11_5004 and Ac3H11_5005 overlap by 39 nucleotides Ac3H11_5005 and Ac3H11_5006 are separated by 312 nucleotides
Ac3H11_5002 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 172,217 to 174,142
_5002
Ac3H11_5003 - putative transmembrane protein, at 174,139 to 174,657
_5003
Ac3H11_5004 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 174,695 to 176,593
_5004
Ac3H11_5005 - hypothetical protein, at 176,555 to 176,671
_5005
Ac3H11_5006 - Mobile element protein, at 176,984 to 178,015
_5006
Position (kb) 174
175
176
177 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.835 kb on + strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.994 kb on + strand at 173.995 kb on - strand at 173.995 kb on - strand at 173.995 kb on - strand at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.670 kb on + strand at 174.670 kb on + strand at 174.705 kb on + strand at 174.705 kb on + strand at 174.705 kb on + strand at 174.706 kb on - strand at 174.723 kb on + strand at 174.724 kb on - strand at 174.724 kb on - strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.931 kb on - strand, within Ac3H11_5004 at 175.060 kb on - strand, within Ac3H11_5004 at 175.307 kb on + strand, within Ac3H11_5004 at 175.345 kb on - strand, within Ac3H11_5004 at 175.362 kb on + strand, within Ac3H11_5004 at 175.410 kb on + strand, within Ac3H11_5004 at 175.521 kb on + strand, within Ac3H11_5004 at 175.522 kb on - strand, within Ac3H11_5004 at 175.522 kb on - strand, within Ac3H11_5004 at 175.522 kb on - strand, within Ac3H11_5004 at 175.522 kb on - strand, within Ac3H11_5004 at 175.560 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.734 kb on + strand, within Ac3H11_5004 at 175.735 kb on - strand, within Ac3H11_5004 at 175.735 kb on - strand, within Ac3H11_5004 at 175.735 kb on - strand, within Ac3H11_5004 at 175.735 kb on - strand, within Ac3H11_5004 at 175.735 kb on - strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.740 kb on + strand, within Ac3H11_5004 at 175.741 kb on - strand, within Ac3H11_5004 at 175.784 kb on - strand, within Ac3H11_5004 at 175.784 kb on - strand, within Ac3H11_5004 at 175.815 kb on + strand, within Ac3H11_5004 at 175.815 kb on + strand, within Ac3H11_5004 at 175.816 kb on - strand, within Ac3H11_5004 at 175.816 kb on - strand, within Ac3H11_5004 at 175.816 kb on - strand, within Ac3H11_5004 at 175.816 kb on - strand, within Ac3H11_5004 at 175.980 kb on + strand, within Ac3H11_5004 at 176.021 kb on - strand, within Ac3H11_5004 at 176.232 kb on + strand, within Ac3H11_5004 at 176.232 kb on + strand, within Ac3H11_5004 at 176.232 kb on + strand, within Ac3H11_5004 at 176.232 kb on + strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.233 kb on - strand, within Ac3H11_5004 at 176.331 kb on + strand, within Ac3H11_5004 at 176.331 kb on + strand, within Ac3H11_5004 at 176.331 kb on + strand, within Ac3H11_5004 at 176.331 kb on + strand, within Ac3H11_5004 at 176.332 kb on - strand, within Ac3H11_5004 at 176.332 kb on - strand, within Ac3H11_5004 at 176.385 kb on + strand, within Ac3H11_5004 at 176.386 kb on - strand, within Ac3H11_5004 at 176.386 kb on - strand, within Ac3H11_5004 at 176.386 kb on - strand, within Ac3H11_5004 at 176.386 kb on - strand, within Ac3H11_5004 at 176.386 kb on - strand, within Ac3H11_5004 at 176.421 kb on + strand at 176.421 kb on + strand at 176.427 kb on + strand at 176.427 kb on + strand at 176.427 kb on + strand at 176.427 kb on + strand at 176.428 kb on - strand at 176.428 kb on - strand at 176.428 kb on - strand at 176.428 kb on - strand at 176.428 kb on - strand at 176.439 kb on + strand at 176.439 kb on + strand at 176.439 kb on + strand at 176.439 kb on + strand at 176.439 kb on + strand at 176.440 kb on - strand at 176.440 kb on - strand at 176.440 kb on - strand at 176.440 kb on - strand at 176.440 kb on - strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.448 kb on + strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.449 kb on - strand at 176.563 kb on - strand at 176.563 kb on - strand at 176.691 kb on - strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.763 kb on + strand at 176.764 kb on - strand at 176.764 kb on - strand at 176.764 kb on - strand at 176.764 kb on - strand at 176.764 kb on - strand at 176.764 kb on - strand at 176.846 kb on - strand at 176.846 kb on - strand at 176.849 kb on + strand at 176.849 kb on + strand at 176.849 kb on + strand at 176.850 kb on - strand at 176.850 kb on - strand at 176.863 kb on - strand at 176.905 kb on + strand at 176.905 kb on + strand at 176.906 kb on - strand at 176.906 kb on - strand at 176.913 kb on + strand at 176.913 kb on + strand at 176.913 kb on + strand at 176.914 kb on - strand at 176.914 kb on - strand at 176.914 kb on - strand at 177.008 kb on - strand at 177.419 kb on + strand, within Ac3H11_5006 at 177.419 kb on + strand, within Ac3H11_5006 at 177.419 kb on + strand, within Ac3H11_5006 at 177.419 kb on + strand, within Ac3H11_5006 at 177.419 kb on + strand, within Ac3H11_5006 at 177.420 kb on - strand, within Ac3H11_5006 at 177.420 kb on - strand, within Ac3H11_5006 at 177.420 kb on - strand, within Ac3H11_5006 at 177.420 kb on - strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.474 kb on + strand, within Ac3H11_5006 at 177.475 kb on - strand, within Ac3H11_5006 at 177.475 kb on - strand, within Ac3H11_5006 at 177.475 kb on - strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.545 kb on + strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006 at 177.546 kb on - strand, within Ac3H11_5006
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 173,805 + Ac3H11_5002 0.82 +1.9 173,805 + Ac3H11_5002 0.82 -0.5 173,805 + Ac3H11_5002 0.82 +2.6 173,805 + Ac3H11_5002 0.82 +2.1 173,805 + Ac3H11_5002 0.82 -3.5 173,806 - Ac3H11_5002 0.83 +1.7 173,806 - Ac3H11_5002 0.83 +1.8 173,806 - Ac3H11_5002 0.83 +0.4 173,806 - Ac3H11_5002 0.83 +0.1 173,806 - Ac3H11_5002 0.83 -0.6 173,835 + Ac3H11_5002 0.84 -3.8 173,836 - Ac3H11_5002 0.84 -2.0 173,836 - Ac3H11_5002 0.84 +0.5 173,836 - Ac3H11_5002 0.84 -0.6 173,994 + +0.4 173,995 - +1.2 173,995 - -2.6 173,995 - +0.5 174,566 + Ac3H11_5003 0.82 -1.1 174,566 + Ac3H11_5003 0.82 -0.6 174,566 + Ac3H11_5003 0.82 -0.3 174,566 + Ac3H11_5003 0.82 -0.3 174,567 - Ac3H11_5003 0.82 -0.2 174,567 - +3.0 174,567 - Ac3H11_5003 0.82 -0.0 174,567 - Ac3H11_5003 0.82 +1.0 174,567 - Ac3H11_5003 0.82 +1.5 174,567 - Ac3H11_5003 0.82 -2.4 174,567 - Ac3H11_5003 0.82 -1.6 174,670 + +0.0 174,670 + -0.4 174,705 + -0.9 174,705 + +2.7 174,705 + -1.2 174,706 - +0.7 174,723 + -0.1 174,724 - +0.5 174,724 - +1.3 174,804 + +1.5 174,804 + -1.2 174,804 + -0.3 174,804 + -1.2 174,804 + +3.2 174,804 + +0.1 174,805 - -0.2 174,805 - +0.6 174,805 - -0.3 174,805 - +2.6 174,805 - +0.9 174,846 + -0.8 174,846 + +1.1 174,846 + -1.3 174,846 + +0.3 174,846 + -3.1 174,846 + +1.0 174,846 + -0.6 174,847 - -0.3 174,847 - +1.5 174,847 - -0.8 174,847 - -0.3 174,847 - +1.6 174,931 - Ac3H11_5004 0.12 -0.4 175,060 - Ac3H11_5004 0.19 -1.6 175,307 + Ac3H11_5004 0.32 -1.1 175,345 - Ac3H11_5004 0.34 -1.0 175,362 + Ac3H11_5004 0.35 +0.2 175,410 + Ac3H11_5004 0.38 -0.1 175,521 + Ac3H11_5004 0.43 -0.5 175,522 - Ac3H11_5004 0.44 -2.5 175,522 - Ac3H11_5004 0.44 +0.7 175,522 - Ac3H11_5004 0.44 -1.9 175,522 - Ac3H11_5004 0.44 -1.4 175,560 + Ac3H11_5004 0.46 -0.8 175,734 + Ac3H11_5004 0.55 -1.2 175,734 + Ac3H11_5004 0.55 +0.5 175,734 + Ac3H11_5004 0.55 -2.2 175,734 + Ac3H11_5004 0.55 -1.1 175,734 + Ac3H11_5004 0.55 +4.4 175,734 + Ac3H11_5004 0.55 -0.8 175,734 + Ac3H11_5004 0.55 +0.6 175,735 - Ac3H11_5004 0.55 -0.1 175,735 - Ac3H11_5004 0.55 -1.8 175,735 - Ac3H11_5004 0.55 +0.7 175,735 - Ac3H11_5004 0.55 -1.6 175,735 - Ac3H11_5004 0.55 -0.1 175,740 + Ac3H11_5004 0.55 -0.4 175,740 + Ac3H11_5004 0.55 +0.5 175,740 + Ac3H11_5004 0.55 -0.2 175,740 + Ac3H11_5004 0.55 -0.7 175,740 + Ac3H11_5004 0.55 +0.3 175,740 + Ac3H11_5004 0.55 -0.6 175,740 + Ac3H11_5004 0.55 +0.4 175,741 - Ac3H11_5004 0.55 -2.2 175,784 - Ac3H11_5004 0.57 -0.6 175,784 - Ac3H11_5004 0.57 -1.2 175,815 + Ac3H11_5004 0.59 -0.9 175,815 + Ac3H11_5004 0.59 -0.2 175,816 - Ac3H11_5004 0.59 -0.9 175,816 - Ac3H11_5004 0.59 +1.0 175,816 - Ac3H11_5004 0.59 -1.4 175,816 - Ac3H11_5004 0.59 -1.8 175,980 + Ac3H11_5004 0.68 -1.2 176,021 - Ac3H11_5004 0.70 +0.2 176,232 + Ac3H11_5004 0.81 +0.8 176,232 + Ac3H11_5004 0.81 +0.3 176,232 + Ac3H11_5004 0.81 -0.2 176,232 + Ac3H11_5004 0.81 -2.1 176,233 - Ac3H11_5004 0.81 +2.2 176,233 - Ac3H11_5004 0.81 -2.2 176,233 - Ac3H11_5004 0.81 +2.0 176,233 - Ac3H11_5004 0.81 +1.2 176,233 - Ac3H11_5004 0.81 -0.3 176,233 - Ac3H11_5004 0.81 +1.8 176,331 + Ac3H11_5004 0.86 +0.9 176,331 + Ac3H11_5004 0.86 +0.8 176,331 + Ac3H11_5004 0.86 +1.3 176,331 + Ac3H11_5004 0.86 -0.6 176,332 - Ac3H11_5004 0.86 -1.3 176,332 - Ac3H11_5004 0.86 -0.2 176,385 + Ac3H11_5004 0.89 -1.1 176,386 - Ac3H11_5004 0.89 -0.3 176,386 - Ac3H11_5004 0.89 -0.8 176,386 - Ac3H11_5004 0.89 -0.3 176,386 - Ac3H11_5004 0.89 +0.9 176,386 - Ac3H11_5004 0.89 +0.8 176,421 + +0.2 176,421 + +2.2 176,427 + -1.1 176,427 + -1.7 176,427 + +1.0 176,427 + +0.2 176,428 - +0.7 176,428 - +4.8 176,428 - +0.2 176,428 - -0.3 176,428 - +0.1 176,439 + -1.1 176,439 + -0.8 176,439 + +0.2 176,439 + -1.0 176,439 + +2.1 176,440 - +1.7 176,440 - +0.6 176,440 - +1.5 176,440 - -1.6 176,440 - -1.4 176,448 + +1.2 176,448 + +0.1 176,448 + -0.3 176,448 + -0.2 176,448 + -0.4 176,448 + +1.0 176,448 + +1.0 176,448 + -1.1 176,448 + +1.3 176,448 + +0.3 176,448 + +2.0 176,448 + -1.8 176,448 + -0.5 176,448 + -0.5 176,449 - -1.4 176,449 - -1.0 176,449 - -0.1 176,449 - -0.5 176,449 - -1.5 176,449 - +0.1 176,449 - -2.7 176,449 - -1.2 176,449 - +1.2 176,449 - -0.7 176,449 - +0.6 176,449 - -1.0 176,449 - -0.5 176,449 - +2.0 176,563 - +0.9 176,563 - +0.5 176,691 - +0.1 176,763 + -0.1 176,763 + -1.2 176,763 + -0.8 176,763 + -0.7 176,763 + -0.1 176,763 + +0.7 176,763 + -1.1 176,764 - -1.6 176,764 - +0.5 176,764 - -0.2 176,764 - -0.1 176,764 - +0.4 176,764 - -1.8 176,846 - +2.4 176,846 - +2.3 176,849 + +0.5 176,849 + +0.6 176,849 + -3.2 176,850 - +1.6 176,850 - +2.1 176,863 - +0.2 176,905 + +1.2 176,905 + -0.3 176,906 - +0.6 176,906 - +2.6 176,913 + +0.5 176,913 + -0.6 176,913 + +0.8 176,914 - -0.4 176,914 - +0.6 176,914 - +0.3 177,008 - +0.4 177,419 + Ac3H11_5006 0.42 -2.4 177,419 + Ac3H11_5006 0.42 -0.3 177,419 + Ac3H11_5006 0.42 +0.7 177,419 + Ac3H11_5006 0.42 +2.0 177,419 + Ac3H11_5006 0.42 -0.4 177,420 - Ac3H11_5006 0.42 +0.2 177,420 - Ac3H11_5006 0.42 +1.5 177,420 - Ac3H11_5006 0.42 +1.1 177,420 - Ac3H11_5006 0.42 -0.6 177,474 + Ac3H11_5006 0.47 -0.1 177,474 + Ac3H11_5006 0.47 +0.2 177,474 + Ac3H11_5006 0.47 +1.8 177,474 + Ac3H11_5006 0.47 -0.7 177,474 + Ac3H11_5006 0.47 -0.5 177,474 + Ac3H11_5006 0.47 +1.6 177,474 + Ac3H11_5006 0.47 -0.8 177,474 + Ac3H11_5006 0.47 -1.9 177,475 - Ac3H11_5006 0.48 +0.1 177,475 - Ac3H11_5006 0.48 -1.2 177,475 - Ac3H11_5006 0.48 +2.1 177,545 + Ac3H11_5006 0.54 +0.6 177,545 + Ac3H11_5006 0.54 +0.4 177,545 + Ac3H11_5006 0.54 -0.2 177,545 + Ac3H11_5006 0.54 +2.6 177,545 + Ac3H11_5006 0.54 +0.5 177,545 + Ac3H11_5006 0.54 -0.5 177,545 + Ac3H11_5006 0.54 +0.7 177,545 + Ac3H11_5006 0.54 -0.7 177,546 - Ac3H11_5006 0.54 -0.4 177,546 - Ac3H11_5006 0.54 -0.4 177,546 - Ac3H11_5006 0.54 -3.5 177,546 - Ac3H11_5006 0.54 -0.2 177,546 - Ac3H11_5006 0.54 +0.8 177,546 - Ac3H11_5006 0.54 +0.4 177,546 - Ac3H11_5006 0.54 +0.6 177,546 - Ac3H11_5006 0.54 +1.0 177,546 - Ac3H11_5006 0.54 -0.5 177,546 - Ac3H11_5006 0.54 -2.3 177,546 - Ac3H11_5006 0.54 -0.2
Or see this region's nucleotide sequence