Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4487 and Ac3H11_4488 are separated by 72 nucleotides Ac3H11_4488 and Ac3H11_4489 are separated by 85 nucleotides
Ac3H11_4487 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 74,905 to 75,825
_4487
Ac3H11_4488 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 75,898 to 77,877
_4488
Ac3H11_4489 - Nitrate/nitrite response regulator protein, at 77,963 to 78,664
_4489
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 75.440 kb on + strand, within Ac3H11_4487 at 75.440 kb on + strand, within Ac3H11_4487 at 75.441 kb on - strand, within Ac3H11_4487 at 75.702 kb on + strand, within Ac3H11_4487 at 75.703 kb on + strand, within Ac3H11_4487 at 75.703 kb on + strand, within Ac3H11_4487 at 75.704 kb on - strand, within Ac3H11_4487 at 75.837 kb on - strand at 75.837 kb on - strand at 75.886 kb on + strand at 75.886 kb on + strand at 75.886 kb on + strand at 75.886 kb on + strand at 75.886 kb on + strand at 75.886 kb on + strand at 75.887 kb on - strand at 75.887 kb on - strand at 75.887 kb on - strand at 75.887 kb on - strand at 75.887 kb on - strand at 75.887 kb on - strand at 76.435 kb on + strand, within Ac3H11_4488 at 76.435 kb on + strand, within Ac3H11_4488 at 76.435 kb on + strand, within Ac3H11_4488 at 76.436 kb on - strand, within Ac3H11_4488 at 76.436 kb on - strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.666 kb on + strand, within Ac3H11_4488 at 76.667 kb on - strand, within Ac3H11_4488 at 76.667 kb on - strand, within Ac3H11_4488 at 76.667 kb on - strand, within Ac3H11_4488 at 76.798 kb on + strand, within Ac3H11_4488 at 76.798 kb on + strand, within Ac3H11_4488 at 76.798 kb on + strand, within Ac3H11_4488 at 76.798 kb on + strand, within Ac3H11_4488 at 76.798 kb on + strand, within Ac3H11_4488 at 76.799 kb on - strand, within Ac3H11_4488 at 76.799 kb on - strand, within Ac3H11_4488 at 76.799 kb on - strand, within Ac3H11_4488 at 76.799 kb on - strand, within Ac3H11_4488 at 76.799 kb on - strand, within Ac3H11_4488 at 77.317 kb on + strand, within Ac3H11_4488 at 77.318 kb on - strand, within Ac3H11_4488 at 77.318 kb on - strand, within Ac3H11_4488 at 77.318 kb on - strand, within Ac3H11_4488 at 77.318 kb on - strand, within Ac3H11_4488 at 77.318 kb on - strand, within Ac3H11_4488 at 77.825 kb on + strand at 77.825 kb on + strand at 77.825 kb on + strand at 77.826 kb on - strand at 77.826 kb on - strand at 77.826 kb on - strand at 77.876 kb on - strand at 77.876 kb on - strand at 77.876 kb on - strand at 78.635 kb on + strand at 78.635 kb on + strand at 78.636 kb on - strand at 78.636 kb on - strand at 78.636 kb on - strand at 78.840 kb on + strand at 78.840 kb on + strand at 78.841 kb on - strand at 78.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 75,440 + Ac3H11_4487 0.58 +2.5 75,440 + Ac3H11_4487 0.58 +3.7 75,441 - Ac3H11_4487 0.58 -2.0 75,702 + Ac3H11_4487 0.87 +0.1 75,703 + Ac3H11_4487 0.87 -1.6 75,703 + Ac3H11_4487 0.87 +1.3 75,704 - Ac3H11_4487 0.87 -1.7 75,837 - +0.0 75,837 - +2.2 75,886 + +0.3 75,886 + +0.4 75,886 + -1.4 75,886 + -1.2 75,886 + -0.3 75,886 + +0.1 75,887 - +0.2 75,887 - +1.3 75,887 - +0.7 75,887 - +0.9 75,887 - -0.9 75,887 - +0.9 76,435 + Ac3H11_4488 0.27 -0.4 76,435 + Ac3H11_4488 0.27 +1.0 76,435 + Ac3H11_4488 0.27 -0.4 76,436 - Ac3H11_4488 0.27 -0.1 76,436 - Ac3H11_4488 0.27 +1.2 76,666 + Ac3H11_4488 0.39 +1.7 76,666 + Ac3H11_4488 0.39 +2.9 76,666 + Ac3H11_4488 0.39 +0.6 76,666 + Ac3H11_4488 0.39 +0.2 76,666 + Ac3H11_4488 0.39 -0.0 76,666 + Ac3H11_4488 0.39 -1.3 76,666 + Ac3H11_4488 0.39 -3.0 76,666 + Ac3H11_4488 0.39 -2.8 76,667 - Ac3H11_4488 0.39 +0.9 76,667 - Ac3H11_4488 0.39 -0.8 76,667 - Ac3H11_4488 0.39 +0.7 76,798 + Ac3H11_4488 0.45 +0.6 76,798 + Ac3H11_4488 0.45 -1.5 76,798 + Ac3H11_4488 0.45 -1.3 76,798 + Ac3H11_4488 0.45 -0.2 76,798 + Ac3H11_4488 0.45 +0.7 76,799 - Ac3H11_4488 0.46 +1.0 76,799 - Ac3H11_4488 0.46 +0.1 76,799 - Ac3H11_4488 0.46 +1.7 76,799 - Ac3H11_4488 0.46 -0.9 76,799 - Ac3H11_4488 0.46 -0.9 77,317 + Ac3H11_4488 0.72 +0.3 77,318 - Ac3H11_4488 0.72 +1.3 77,318 - Ac3H11_4488 0.72 +0.5 77,318 - Ac3H11_4488 0.72 -1.9 77,318 - Ac3H11_4488 0.72 +0.7 77,318 - Ac3H11_4488 0.72 +1.0 77,825 + +0.2 77,825 + -0.0 77,825 + +0.8 77,826 - -0.3 77,826 - -1.8 77,826 - -0.6 77,876 - +0.3 77,876 - +1.1 77,876 - +0.1 78,635 + -0.5 78,635 + -1.0 78,636 - -2.0 78,636 - -1.2 78,636 - -1.6 78,840 + -1.5 78,840 + -0.2 78,841 - -1.9 78,841 - -1.0
Or see this region's nucleotide sequence