Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3944 and Ac3H11_3945 are separated by 376 nucleotides Ac3H11_3945 and Ac3H11_3946 overlap by 1 nucleotides Ac3H11_3946 and Ac3H11_3947 overlap by 20 nucleotides Ac3H11_3947 and Ac3H11_3948 overlap by 105 nucleotides
Ac3H11_3944 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 166,426 to 167,424
_3944
Ac3H11_3945 - Sensory histidine kinase QseC, at 167,801 to 169,138
_3945
Ac3H11_3946 - Two-component system response regulator QseB, at 169,138 to 169,797
_3946
Ac3H11_3947 - hypothetical protein, at 169,778 to 169,900
_3947
Ac3H11_3948 - Ferrichrome-iron receptor, at 169,796 to 172,105
_3948
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.977 kb on - strand, within Ac3H11_3944 at 167.024 kb on + strand, within Ac3H11_3944 at 167.025 kb on - strand, within Ac3H11_3944 at 167.338 kb on + strand at 167.338 kb on + strand at 167.339 kb on - strand at 167.339 kb on - strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.384 kb on + strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.385 kb on - strand at 167.428 kb on + strand at 167.428 kb on + strand at 167.428 kb on + strand at 167.437 kb on - strand at 167.437 kb on - strand at 167.611 kb on + strand at 167.612 kb on - strand at 167.612 kb on - strand at 167.725 kb on - strand at 167.766 kb on + strand at 167.777 kb on + strand at 167.777 kb on + strand at 167.796 kb on + strand at 167.797 kb on - strand at 167.806 kb on + strand at 168.069 kb on + strand, within Ac3H11_3945 at 168.069 kb on + strand, within Ac3H11_3945 at 168.069 kb on + strand, within Ac3H11_3945 at 168.070 kb on - strand, within Ac3H11_3945 at 168.070 kb on - strand, within Ac3H11_3945 at 168.982 kb on - strand, within Ac3H11_3945 at 168.982 kb on - strand, within Ac3H11_3945 at 169.220 kb on + strand, within Ac3H11_3946 at 169.220 kb on + strand, within Ac3H11_3946 at 169.220 kb on + strand, within Ac3H11_3946 at 169.221 kb on - strand, within Ac3H11_3946 at 169.221 kb on - strand, within Ac3H11_3946 at 169.418 kb on + strand, within Ac3H11_3946 at 169.419 kb on - strand, within Ac3H11_3946 at 169.505 kb on + strand, within Ac3H11_3946 at 169.506 kb on - strand, within Ac3H11_3946 at 169.506 kb on - strand, within Ac3H11_3946 at 169.665 kb on - strand, within Ac3H11_3946 at 169.724 kb on + strand, within Ac3H11_3946 at 169.725 kb on - strand, within Ac3H11_3946 at 169.809 kb on - strand at 169.809 kb on - strand at 169.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 166,977 - Ac3H11_3944 0.55 -1.5 167,024 + Ac3H11_3944 0.60 -1.8 167,025 - Ac3H11_3944 0.60 -1.3 167,338 + -0.1 167,338 + -1.9 167,339 - +0.2 167,339 - -0.9 167,384 + +1.3 167,384 + -1.8 167,384 + -0.1 167,384 + -2.2 167,384 + -0.6 167,384 + +0.4 167,384 + +0.3 167,384 + +1.8 167,385 - +0.9 167,385 - -0.9 167,385 - -0.9 167,385 - -0.3 167,428 + +1.1 167,428 + -1.4 167,428 + -0.0 167,437 - -0.1 167,437 - +0.4 167,611 + +0.6 167,612 - +0.4 167,612 - -0.9 167,725 - +0.8 167,766 + -2.1 167,777 + +1.8 167,777 + -0.1 167,796 + -2.6 167,797 - +0.4 167,806 + -0.3 168,069 + Ac3H11_3945 0.20 +0.8 168,069 + Ac3H11_3945 0.20 -1.2 168,069 + Ac3H11_3945 0.20 -0.7 168,070 - Ac3H11_3945 0.20 +0.4 168,070 - Ac3H11_3945 0.20 +0.1 168,982 - Ac3H11_3945 0.88 -0.7 168,982 - Ac3H11_3945 0.88 -1.5 169,220 + Ac3H11_3946 0.12 +0.5 169,220 + Ac3H11_3946 0.12 -2.8 169,220 + Ac3H11_3946 0.12 -1.4 169,221 - Ac3H11_3946 0.13 +1.1 169,221 - Ac3H11_3946 0.13 -0.9 169,418 + Ac3H11_3946 0.42 +0.0 169,419 - Ac3H11_3946 0.43 +0.6 169,505 + Ac3H11_3946 0.56 +0.4 169,506 - Ac3H11_3946 0.56 +0.1 169,506 - Ac3H11_3946 0.56 -0.3 169,665 - Ac3H11_3946 0.80 +0.4 169,724 + Ac3H11_3946 0.89 +0.5 169,725 - Ac3H11_3946 0.89 -0.9 169,809 - +1.1 169,809 - -0.4 169,913 + -2.2
Or see this region's nucleotide sequence