Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3698 and Ac3H11_3699 are separated by 9 nucleotides Ac3H11_3699 and Ac3H11_3700 are separated by 20 nucleotides Ac3H11_3700 and Ac3H11_3701 are separated by 32 nucleotides Ac3H11_3701 and Ac3H11_3702 are separated by 213 nucleotides
Ac3H11_3698 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 56,431 to 57,483
_3698
Ac3H11_3699 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 57,493 to 57,957
_3699
Ac3H11_3700 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 57,978 to 60,230
_3700
Ac3H11_3701 - hypothetical protein, at 60,263 to 60,382
_3701
Ac3H11_3702 - Urea ABC transporter, urea binding protein, at 60,596 to 61,858
_3702
Position (kb)
57
58
59
60
61 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 56.986 kb on + strand, within Ac3H11_3698 at 56.986 kb on + strand, within Ac3H11_3698 at 56.987 kb on - strand, within Ac3H11_3698 at 56.987 kb on - strand, within Ac3H11_3698 at 57.409 kb on + strand at 57.409 kb on + strand at 57.410 kb on - strand at 57.410 kb on - strand at 57.410 kb on - strand at 57.481 kb on + strand at 57.481 kb on + strand at 57.548 kb on + strand, within Ac3H11_3699 at 57.549 kb on - strand, within Ac3H11_3699 at 58.843 kb on + strand, within Ac3H11_3700 at 58.843 kb on + strand, within Ac3H11_3700 at 58.936 kb on + strand, within Ac3H11_3700 at 58.936 kb on + strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 58.937 kb on - strand, within Ac3H11_3700 at 59.381 kb on - strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.947 kb on + strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 59.948 kb on - strand, within Ac3H11_3700 at 60.374 kb on - strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.383 kb on + strand at 60.398 kb on + strand at 60.480 kb on - strand at 60.524 kb on + strand at 60.524 kb on + strand at 60.524 kb on + strand at 60.525 kb on - strand at 60.576 kb on + strand at 60.576 kb on + strand at 60.576 kb on + strand at 60.577 kb on - strand at 60.577 kb on - strand at 60.577 kb on - strand at 60.608 kb on + strand at 60.609 kb on - strand at 60.984 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.017 kb on - strand, within Ac3H11_3702 at 61.062 kb on - strand, within Ac3H11_3702 at 61.118 kb on + strand, within Ac3H11_3702 at 61.118 kb on + strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.119 kb on - strand, within Ac3H11_3702 at 61.157 kb on + strand, within Ac3H11_3702 at 61.157 kb on + strand, within Ac3H11_3702 at 61.202 kb on + strand, within Ac3H11_3702 at 61.202 kb on + strand, within Ac3H11_3702 at 61.203 kb on - strand, within Ac3H11_3702 at 61.203 kb on - strand, within Ac3H11_3702 at 61.227 kb on - strand, within Ac3H11_3702
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 56,986 + Ac3H11_3698 0.53 +0.1 56,986 + Ac3H11_3698 0.53 +0.8 56,987 - Ac3H11_3698 0.53 +1.9 56,987 - Ac3H11_3698 0.53 -0.3 57,409 + -0.1 57,409 + -1.1 57,410 - +0.5 57,410 - -0.7 57,410 - -0.7 57,481 + -1.0 57,481 + -1.4 57,548 + Ac3H11_3699 0.12 -1.0 57,549 - Ac3H11_3699 0.12 -3.4 58,843 + Ac3H11_3700 0.38 +0.4 58,843 + Ac3H11_3700 0.38 +1.9 58,936 + Ac3H11_3700 0.43 -0.8 58,936 + Ac3H11_3700 0.43 -0.1 58,937 - Ac3H11_3700 0.43 -0.1 58,937 - Ac3H11_3700 0.43 +0.3 58,937 - Ac3H11_3700 0.43 +0.4 58,937 - Ac3H11_3700 0.43 +0.1 59,381 - Ac3H11_3700 0.62 +1.6 59,947 + Ac3H11_3700 0.87 +3.1 59,947 + Ac3H11_3700 0.87 +0.5 59,947 + Ac3H11_3700 0.87 -0.6 59,948 - Ac3H11_3700 0.87 -1.2 59,948 - Ac3H11_3700 0.87 -0.1 59,948 - Ac3H11_3700 0.87 -0.1 59,948 - Ac3H11_3700 0.87 -0.2 59,948 - Ac3H11_3700 0.87 -3.1 60,374 - -1.3 60,383 + -0.8 60,383 + -1.8 60,383 + +1.7 60,383 + +0.9 60,398 + -2.8 60,480 - +0.3 60,524 + -0.5 60,524 + -0.5 60,524 + -1.0 60,525 - -0.2 60,576 + +0.2 60,576 + +0.6 60,576 + -1.5 60,577 - +1.2 60,577 - -1.1 60,577 - -1.5 60,608 + -1.2 60,609 - +2.6 60,984 - Ac3H11_3702 0.31 -1.0 61,017 - Ac3H11_3702 0.33 -0.7 61,017 - Ac3H11_3702 0.33 +0.6 61,017 - Ac3H11_3702 0.33 -0.7 61,017 - Ac3H11_3702 0.33 +0.2 61,017 - Ac3H11_3702 0.33 +0.8 61,062 - Ac3H11_3702 0.37 +1.7 61,118 + Ac3H11_3702 0.41 -0.8 61,118 + Ac3H11_3702 0.41 -3.0 61,119 - Ac3H11_3702 0.41 -0.3 61,119 - Ac3H11_3702 0.41 -2.3 61,119 - Ac3H11_3702 0.41 +0.6 61,119 - Ac3H11_3702 0.41 +1.6 61,157 + Ac3H11_3702 0.44 +0.3 61,157 + Ac3H11_3702 0.44 -1.4 61,202 + Ac3H11_3702 0.48 -0.8 61,202 + Ac3H11_3702 0.48 -2.4 61,203 - Ac3H11_3702 0.48 -0.7 61,203 - Ac3H11_3702 0.48 +0.4 61,227 - Ac3H11_3702 0.50 +1.7
Or see this region's nucleotide sequence