Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1813 and Ac3H11_1814 are separated by 66 nucleotides Ac3H11_1814 and Ac3H11_1815 overlap by 17 nucleotides Ac3H11_1815 and Ac3H11_1816 are separated by 28 nucleotides
Ac3H11_1813 - CRISPR-associated protein Cas1, at 18,717 to 19,724
_1813
Ac3H11_1814 - low-specificity D-threonine aldolase, at 19,791 to 20,960
_1814
Ac3H11_1815 - Phosphoglycerate mutase (EC 5.4.2.1), at 20,944 to 21,585
_1815
Ac3H11_1816 - Probable lipoprotein transmembrane, at 21,614 to 22,228
_1816
Position (kb) 19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 19.018 kb on + strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.091 kb on - strand, within Ac3H11_1813 at 19.110 kb on + strand, within Ac3H11_1813 at 19.201 kb on - strand, within Ac3H11_1813 at 19.201 kb on - strand, within Ac3H11_1813 at 19.260 kb on + strand, within Ac3H11_1813 at 19.260 kb on + strand, within Ac3H11_1813 at 19.261 kb on - strand, within Ac3H11_1813 at 19.300 kb on - strand, within Ac3H11_1813 at 19.300 kb on - strand, within Ac3H11_1813 at 19.363 kb on + strand, within Ac3H11_1813 at 19.363 kb on + strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.379 kb on - strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.567 kb on + strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.580 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.602 kb on + strand, within Ac3H11_1813 at 19.642 kb on + strand at 19.642 kb on + strand at 19.643 kb on - strand at 19.643 kb on - strand at 19.643 kb on - strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.646 kb on - strand at 19.681 kb on - strand at 19.747 kb on - strand at 19.776 kb on + strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.966 kb on + strand, within Ac3H11_1814 at 19.999 kb on + strand, within Ac3H11_1814 at 20.396 kb on - strand, within Ac3H11_1814 at 20.396 kb on - strand, within Ac3H11_1814 at 21.108 kb on - strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.709 kb on - strand, within Ac3H11_1816 at 21.759 kb on + strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.903 kb on + strand, within Ac3H11_1816 at 21.903 kb on + strand at 21.903 kb on + strand, within Ac3H11_1816 at 21.904 kb on - strand, within Ac3H11_1816 at 21.912 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 19,018 + Ac3H11_1813 0.30 -2.5 19,019 - Ac3H11_1813 0.30 -1.7 19,019 - Ac3H11_1813 0.30 -1.3 19,019 - Ac3H11_1813 0.30 +1.7 19,091 - Ac3H11_1813 0.37 +1.6 19,110 + Ac3H11_1813 0.39 -0.8 19,201 - Ac3H11_1813 0.48 -2.3 19,201 - Ac3H11_1813 0.48 -0.7 19,260 + Ac3H11_1813 0.54 +0.5 19,260 + Ac3H11_1813 0.54 +0.2 19,261 - Ac3H11_1813 0.54 -0.4 19,300 - Ac3H11_1813 0.58 +1.0 19,300 - Ac3H11_1813 0.58 +0.2 19,363 + Ac3H11_1813 0.64 -3.5 19,363 + Ac3H11_1813 0.64 -2.7 19,364 - Ac3H11_1813 0.64 -1.5 19,364 - Ac3H11_1813 0.64 +2.8 19,364 - Ac3H11_1813 0.64 -1.6 19,364 - Ac3H11_1813 0.64 +0.3 19,364 - Ac3H11_1813 0.64 +0.7 19,379 - Ac3H11_1813 0.66 -1.7 19,511 + Ac3H11_1813 0.79 +2.4 19,511 + Ac3H11_1813 0.79 -0.1 19,511 + Ac3H11_1813 0.79 +1.5 19,511 + Ac3H11_1813 0.79 -2.0 19,511 + Ac3H11_1813 0.79 -3.0 19,511 + Ac3H11_1813 0.79 -0.6 19,511 + Ac3H11_1813 0.79 -3.2 19,511 + Ac3H11_1813 0.79 -0.3 19,512 - Ac3H11_1813 0.79 -0.4 19,512 - Ac3H11_1813 0.79 +2.3 19,512 - Ac3H11_1813 0.79 +0.7 19,512 - Ac3H11_1813 0.79 -2.0 19,512 - Ac3H11_1813 0.79 +0.6 19,512 - Ac3H11_1813 0.79 -0.7 19,512 - Ac3H11_1813 0.79 +0.5 19,512 - Ac3H11_1813 0.79 +2.6 19,512 - Ac3H11_1813 0.79 -0.9 19,512 - Ac3H11_1813 0.79 -0.7 19,567 + Ac3H11_1813 0.84 -3.3 19,568 - Ac3H11_1813 0.84 +1.8 19,568 - Ac3H11_1813 0.84 +0.8 19,568 - Ac3H11_1813 0.84 +0.6 19,580 + Ac3H11_1813 0.86 +1.2 19,597 + Ac3H11_1813 0.87 -0.5 19,597 + Ac3H11_1813 0.87 +0.3 19,597 + Ac3H11_1813 0.87 -0.1 19,597 + Ac3H11_1813 0.87 -2.0 19,597 + Ac3H11_1813 0.87 -0.1 19,597 + Ac3H11_1813 0.87 -1.9 19,598 - Ac3H11_1813 0.87 +0.0 19,598 - Ac3H11_1813 0.87 -1.2 19,598 - Ac3H11_1813 0.87 -1.8 19,598 - Ac3H11_1813 0.87 -0.1 19,598 - Ac3H11_1813 0.87 +0.1 19,598 - Ac3H11_1813 0.87 +0.2 19,598 - Ac3H11_1813 0.87 +3.8 19,598 - Ac3H11_1813 0.87 +1.6 19,598 - Ac3H11_1813 0.87 -1.3 19,598 - Ac3H11_1813 0.87 +0.2 19,598 - Ac3H11_1813 0.87 -2.7 19,602 + Ac3H11_1813 0.88 -0.8 19,642 + -0.0 19,642 + +0.7 19,643 - -0.6 19,643 - +1.3 19,643 - -0.0 19,645 + -0.9 19,645 + +0.7 19,645 + -0.7 19,645 + -2.6 19,645 + -1.5 19,646 - -0.6 19,681 - -0.5 19,747 - +1.4 19,776 + -1.4 19,777 - +0.8 19,777 - +1.2 19,966 + Ac3H11_1814 0.15 +0.7 19,999 + Ac3H11_1814 0.18 -1.3 20,396 - Ac3H11_1814 0.52 -1.4 20,396 - Ac3H11_1814 0.52 +2.8 21,108 - Ac3H11_1815 0.26 -0.1 21,239 + Ac3H11_1815 0.46 +0.7 21,239 + Ac3H11_1815 0.46 +2.8 21,239 + Ac3H11_1815 0.46 -2.5 21,240 - Ac3H11_1815 0.46 +0.8 21,240 - Ac3H11_1815 0.46 -1.9 21,240 - Ac3H11_1815 0.46 +1.0 21,425 + Ac3H11_1815 0.75 +1.3 21,425 + Ac3H11_1815 0.75 -0.7 21,425 + Ac3H11_1815 0.75 +1.0 21,426 - Ac3H11_1815 0.75 +0.2 21,426 - Ac3H11_1815 0.75 -0.1 21,426 - Ac3H11_1815 0.75 -2.3 21,426 - Ac3H11_1815 0.75 +0.2 21,426 - Ac3H11_1815 0.75 -2.4 21,426 - Ac3H11_1815 0.75 +1.2 21,426 - Ac3H11_1815 0.75 -1.3 21,426 - Ac3H11_1815 0.75 +1.2 21,426 - Ac3H11_1815 0.75 +2.7 21,426 - Ac3H11_1815 0.75 -0.2 21,426 - Ac3H11_1815 0.75 -1.2 21,426 - Ac3H11_1815 0.75 +0.7 21,708 + Ac3H11_1816 0.15 -0.5 21,708 + Ac3H11_1816 0.15 +3.6 21,708 + Ac3H11_1816 0.15 -1.7 21,708 + Ac3H11_1816 0.15 -1.0 21,708 + Ac3H11_1816 0.15 -0.6 21,708 + Ac3H11_1816 0.15 +0.8 21,709 - Ac3H11_1816 0.15 -1.0 21,759 + Ac3H11_1816 0.24 -1.9 21,760 - Ac3H11_1816 0.24 -1.1 21,760 - Ac3H11_1816 0.24 +0.7 21,903 + Ac3H11_1816 0.47 +0.5 21,903 + +1.8 21,903 + Ac3H11_1816 0.47 +1.2 21,904 - Ac3H11_1816 0.47 -0.3 21,912 + Ac3H11_1816 0.48 -0.9 21,930 + Ac3H11_1816 0.51 -1.7 21,930 + Ac3H11_1816 0.51 +0.0 21,931 - Ac3H11_1816 0.52 +0.4 21,931 - Ac3H11_1816 0.52 -1.3
Or see this region's nucleotide sequence