Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1766 and Ac3H11_1779 are separated by 94 nucleotides Ac3H11_1779 and Ac3H11_1767 are separated by 74 nucleotides Ac3H11_1767 and Ac3H11_1768 are separated by 98 nucleotides
Ac3H11_1766 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 379,923 to 381,038
_1766
Ac3H11_1779 - tRNA-Ser-CGA, at 381,133 to 381,219
_1779
Ac3H11_1767 - L-lactate dehydrogenase (EC 1.1.2.3), at 381,294 to 382,553
_1767
Ac3H11_1768 - LysR family transcriptional regulator y2377 (Yersinia pestis KIM), at 382,652 to 383,551
_1768
Position (kb) 381
382
383 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 380.463 kb on + strand, within Ac3H11_1766 at 380.463 kb on + strand, within Ac3H11_1766 at 380.463 kb on + strand, within Ac3H11_1766 at 380.464 kb on - strand, within Ac3H11_1766 at 380.464 kb on - strand, within Ac3H11_1766 at 380.550 kb on + strand, within Ac3H11_1766 at 380.550 kb on + strand, within Ac3H11_1766 at 380.551 kb on - strand, within Ac3H11_1766 at 380.640 kb on + strand, within Ac3H11_1766 at 380.994 kb on + strand at 380.995 kb on - strand at 381.044 kb on + strand at 381.044 kb on + strand at 381.045 kb on - strand at 381.045 kb on - strand at 381.045 kb on - strand at 381.437 kb on - strand, within Ac3H11_1767 at 381.437 kb on - strand, within Ac3H11_1767 at 381.437 kb on - strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.972 kb on + strand, within Ac3H11_1767 at 381.973 kb on - strand, within Ac3H11_1767 at 381.973 kb on - strand, within Ac3H11_1767 at 382.057 kb on - strand, within Ac3H11_1767 at 382.060 kb on + strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.061 kb on - strand, within Ac3H11_1767 at 382.237 kb on + strand, within Ac3H11_1767 at 382.238 kb on - strand, within Ac3H11_1767 at 382.582 kb on + strand at 382.582 kb on + strand at 382.583 kb on - strand at 382.614 kb on - strand at 382.614 kb on - strand at 382.618 kb on - strand at 383.053 kb on - strand, within Ac3H11_1768 at 383.137 kb on - strand, within Ac3H11_1768 at 383.444 kb on + strand, within Ac3H11_1768 at 383.444 kb on + strand, within Ac3H11_1768 at 383.444 kb on + strand, within Ac3H11_1768 at 383.450 kb on + strand, within Ac3H11_1768 at 383.450 kb on + strand, within Ac3H11_1768 at 383.451 kb on - strand, within Ac3H11_1768 at 383.451 kb on - strand, within Ac3H11_1768
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 380,463 + Ac3H11_1766 0.48 +2.5 380,463 + Ac3H11_1766 0.48 -0.2 380,463 + Ac3H11_1766 0.48 +0.1 380,464 - Ac3H11_1766 0.48 -0.1 380,464 - Ac3H11_1766 0.48 +1.9 380,550 + Ac3H11_1766 0.56 +1.6 380,550 + Ac3H11_1766 0.56 -0.3 380,551 - Ac3H11_1766 0.56 +1.7 380,640 + Ac3H11_1766 0.64 -2.6 380,994 + +1.3 380,995 - +1.2 381,044 + -0.1 381,044 + +0.7 381,045 - -1.2 381,045 - +0.6 381,045 - -0.2 381,437 - Ac3H11_1767 0.11 +0.5 381,437 - Ac3H11_1767 0.11 +2.2 381,437 - Ac3H11_1767 0.11 +0.2 381,972 + Ac3H11_1767 0.54 -1.4 381,972 + Ac3H11_1767 0.54 +0.9 381,972 + Ac3H11_1767 0.54 -0.6 381,972 + Ac3H11_1767 0.54 +0.2 381,972 + Ac3H11_1767 0.54 +0.8 381,972 + Ac3H11_1767 0.54 +1.9 381,973 - Ac3H11_1767 0.54 -0.8 381,973 - Ac3H11_1767 0.54 -0.6 382,057 - Ac3H11_1767 0.61 -2.8 382,060 + Ac3H11_1767 0.61 -2.1 382,061 - Ac3H11_1767 0.61 +0.9 382,061 - Ac3H11_1767 0.61 -0.1 382,061 - Ac3H11_1767 0.61 -0.5 382,061 - Ac3H11_1767 0.61 +0.5 382,061 - Ac3H11_1767 0.61 +0.7 382,061 - Ac3H11_1767 0.61 -1.7 382,237 + Ac3H11_1767 0.75 -1.0 382,238 - Ac3H11_1767 0.75 +0.6 382,582 + +0.6 382,582 + -0.5 382,583 - -0.2 382,614 - -2.6 382,614 - -1.0 382,618 - -0.1 383,053 - Ac3H11_1768 0.45 +0.1 383,137 - Ac3H11_1768 0.54 -1.2 383,444 + Ac3H11_1768 0.88 -1.3 383,444 + Ac3H11_1768 0.88 -2.0 383,444 + Ac3H11_1768 0.88 +0.0 383,450 + Ac3H11_1768 0.89 +0.2 383,450 + Ac3H11_1768 0.89 -2.1 383,451 - Ac3H11_1768 0.89 -0.1 383,451 - Ac3H11_1768 0.89 +3.1
Or see this region's nucleotide sequence