Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1610 and Ac3H11_1611 overlap by 4 nucleotides Ac3H11_1611 and Ac3H11_1612 are separated by 5 nucleotides Ac3H11_1612 and Ac3H11_1613 overlap by 4 nucleotides Ac3H11_1613 and Ac3H11_1614 overlap by 4 nucleotides
Ac3H11_1610 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 193,708 to 194,796
_1610
Ac3H11_1611 - ABC transporter, permease protein, at 194,793 to 195,692
_1611
Ac3H11_1612 - ABC transporter, permease protein, at 195,698 to 196,492
_1612
Ac3H11_1613 - Opine oxidase subunit C, at 196,489 to 196,833
_1613
Ac3H11_1614 - Opine oxidase subunit A, at 196,830 to 198,236
_1614
Position (kb)
195
196
197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 194.899 kb on + strand, within Ac3H11_1611 at 194.997 kb on + strand, within Ac3H11_1611 at 195.021 kb on + strand, within Ac3H11_1611 at 195.021 kb on + strand, within Ac3H11_1611 at 195.021 kb on + strand, within Ac3H11_1611 at 195.022 kb on - strand, within Ac3H11_1611 at 195.022 kb on - strand, within Ac3H11_1611 at 195.024 kb on + strand, within Ac3H11_1611 at 195.025 kb on - strand, within Ac3H11_1611 at 195.025 kb on - strand, within Ac3H11_1611 at 195.025 kb on - strand, within Ac3H11_1611 at 195.105 kb on + strand, within Ac3H11_1611 at 195.105 kb on + strand, within Ac3H11_1611 at 195.106 kb on - strand, within Ac3H11_1611 at 195.106 kb on - strand, within Ac3H11_1611 at 195.106 kb on - strand, within Ac3H11_1611 at 195.106 kb on - strand, within Ac3H11_1611 at 195.106 kb on - strand, within Ac3H11_1611 at 195.279 kb on + strand, within Ac3H11_1611 at 195.279 kb on + strand, within Ac3H11_1611 at 195.280 kb on - strand, within Ac3H11_1611 at 195.558 kb on + strand, within Ac3H11_1611 at 195.558 kb on + strand, within Ac3H11_1611 at 195.568 kb on - strand, within Ac3H11_1611 at 195.648 kb on + strand at 195.648 kb on + strand at 195.648 kb on + strand at 195.648 kb on + strand at 195.649 kb on - strand at 196.005 kb on + strand, within Ac3H11_1612 at 196.128 kb on - strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.358 kb on + strand, within Ac3H11_1612 at 196.359 kb on - strand, within Ac3H11_1612 at 196.359 kb on - strand, within Ac3H11_1612 at 196.359 kb on - strand, within Ac3H11_1612 at 196.364 kb on + strand, within Ac3H11_1612 at 196.364 kb on + strand, within Ac3H11_1612 at 196.365 kb on - strand, within Ac3H11_1612 at 196.365 kb on - strand, within Ac3H11_1612 at 196.365 kb on - strand, within Ac3H11_1612 at 196.365 kb on - strand, within Ac3H11_1612 at 196.365 kb on - strand, within Ac3H11_1612 at 196.458 kb on - strand at 196.966 kb on - strand at 197.010 kb on + strand, within Ac3H11_1614 at 197.166 kb on + strand, within Ac3H11_1614 at 197.166 kb on + strand, within Ac3H11_1614 at 197.166 kb on + strand, within Ac3H11_1614 at 197.167 kb on - strand, within Ac3H11_1614 at 197.242 kb on - strand, within Ac3H11_1614 at 197.242 kb on - strand, within Ac3H11_1614 at 197.331 kb on + strand, within Ac3H11_1614 at 197.331 kb on + strand, within Ac3H11_1614 at 197.332 kb on - strand, within Ac3H11_1614 at 197.332 kb on - strand, within Ac3H11_1614 at 197.344 kb on - strand, within Ac3H11_1614 at 197.349 kb on + strand, within Ac3H11_1614 at 197.463 kb on + strand, within Ac3H11_1614 at 197.463 kb on + strand, within Ac3H11_1614 at 197.463 kb on + strand, within Ac3H11_1614 at 197.464 kb on - strand, within Ac3H11_1614 at 197.464 kb on - strand, within Ac3H11_1614 at 197.464 kb on - strand, within Ac3H11_1614 at 197.464 kb on - strand, within Ac3H11_1614 at 197.464 kb on - strand, within Ac3H11_1614 at 197.466 kb on + strand, within Ac3H11_1614 at 197.467 kb on - strand, within Ac3H11_1614
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 194,899 + Ac3H11_1611 0.12 +0.2 194,997 + Ac3H11_1611 0.23 -2.4 195,021 + Ac3H11_1611 0.25 +3.0 195,021 + Ac3H11_1611 0.25 +0.8 195,021 + Ac3H11_1611 0.25 -1.5 195,022 - Ac3H11_1611 0.25 -1.2 195,022 - Ac3H11_1611 0.25 -0.0 195,024 + Ac3H11_1611 0.26 -1.9 195,025 - Ac3H11_1611 0.26 -1.0 195,025 - Ac3H11_1611 0.26 -0.8 195,025 - Ac3H11_1611 0.26 +0.9 195,105 + Ac3H11_1611 0.35 -2.6 195,105 + Ac3H11_1611 0.35 +1.4 195,106 - Ac3H11_1611 0.35 +2.0 195,106 - Ac3H11_1611 0.35 +0.6 195,106 - Ac3H11_1611 0.35 -0.6 195,106 - Ac3H11_1611 0.35 -0.4 195,106 - Ac3H11_1611 0.35 +1.6 195,279 + Ac3H11_1611 0.54 +1.5 195,279 + Ac3H11_1611 0.54 -1.4 195,280 - Ac3H11_1611 0.54 -0.1 195,558 + Ac3H11_1611 0.85 +1.8 195,558 + Ac3H11_1611 0.85 +0.6 195,568 - Ac3H11_1611 0.86 -0.1 195,648 + +1.2 195,648 + +1.4 195,648 + -0.8 195,648 + +0.0 195,649 - -0.4 196,005 + Ac3H11_1612 0.39 -0.1 196,128 - Ac3H11_1612 0.54 -0.6 196,358 + Ac3H11_1612 0.83 +0.9 196,358 + Ac3H11_1612 0.83 -0.5 196,358 + Ac3H11_1612 0.83 -0.6 196,358 + Ac3H11_1612 0.83 +1.4 196,358 + Ac3H11_1612 0.83 -0.3 196,358 + Ac3H11_1612 0.83 -1.3 196,358 + Ac3H11_1612 0.83 -0.7 196,358 + Ac3H11_1612 0.83 -1.7 196,359 - Ac3H11_1612 0.83 -2.5 196,359 - Ac3H11_1612 0.83 -0.4 196,359 - Ac3H11_1612 0.83 +1.0 196,364 + Ac3H11_1612 0.84 -1.6 196,364 + Ac3H11_1612 0.84 -2.4 196,365 - Ac3H11_1612 0.84 +0.7 196,365 - Ac3H11_1612 0.84 -0.6 196,365 - Ac3H11_1612 0.84 +0.3 196,365 - Ac3H11_1612 0.84 +0.2 196,365 - Ac3H11_1612 0.84 +0.5 196,458 - +0.0 196,966 - -0.0 197,010 + Ac3H11_1614 0.13 -2.2 197,166 + Ac3H11_1614 0.24 +1.0 197,166 + Ac3H11_1614 0.24 -0.4 197,166 + Ac3H11_1614 0.24 +0.6 197,167 - Ac3H11_1614 0.24 -3.3 197,242 - Ac3H11_1614 0.29 -0.2 197,242 - Ac3H11_1614 0.29 -0.9 197,331 + Ac3H11_1614 0.36 -0.8 197,331 + Ac3H11_1614 0.36 -0.9 197,332 - Ac3H11_1614 0.36 -0.4 197,332 - Ac3H11_1614 0.36 -0.3 197,344 - Ac3H11_1614 0.37 +0.4 197,349 + Ac3H11_1614 0.37 +1.1 197,463 + Ac3H11_1614 0.45 -0.6 197,463 + Ac3H11_1614 0.45 +0.4 197,463 + Ac3H11_1614 0.45 -2.2 197,464 - Ac3H11_1614 0.45 +0.1 197,464 - Ac3H11_1614 0.45 -1.0 197,464 - Ac3H11_1614 0.45 +3.0 197,464 - Ac3H11_1614 0.45 -2.1 197,464 - Ac3H11_1614 0.45 -0.4 197,466 + Ac3H11_1614 0.45 -1.8 197,467 - Ac3H11_1614 0.45 +0.2
Or see this region's nucleotide sequence