Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_137 and Ac3H11_138 are separated by 36 nucleotides Ac3H11_138 and Ac3H11_139 are separated by 17 nucleotides Ac3H11_139 and Ac3H11_140 are separated by 3 nucleotides
Ac3H11_137 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 20,789 to 22,075
_137
Ac3H11_138 - Gluconolactonase, at 22,112 to 23,035
_138
Ac3H11_139 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 23,053 to 23,955
_139
Ac3H11_140 - Tricarboxylate transport protein TctC, at 23,959 to 24,915
_140
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 21.359 kb on + strand, within Ac3H11_137 at 21.359 kb on + strand, within Ac3H11_137 at 21.659 kb on + strand, within Ac3H11_137 at 21.659 kb on + strand, within Ac3H11_137 at 21.660 kb on - strand, within Ac3H11_137 at 21.660 kb on - strand, within Ac3H11_137 at 21.953 kb on + strand at 21.954 kb on - strand at 21.954 kb on - strand at 21.954 kb on - strand at 21.954 kb on - strand at 21.959 kb on + strand at 21.959 kb on + strand at 21.959 kb on + strand at 21.959 kb on + strand at 21.959 kb on + strand at 21.959 kb on + strand at 21.960 kb on - strand at 21.960 kb on - strand at 21.960 kb on - strand at 21.960 kb on - strand at 21.960 kb on - strand at 22.073 kb on + strand at 22.305 kb on + strand, within Ac3H11_138 at 22.305 kb on + strand, within Ac3H11_138 at 22.306 kb on - strand, within Ac3H11_138 at 22.306 kb on - strand, within Ac3H11_138 at 22.306 kb on - strand, within Ac3H11_138 at 22.306 kb on - strand, within Ac3H11_138 at 22.470 kb on + strand, within Ac3H11_138 at 22.470 kb on + strand, within Ac3H11_138 at 22.471 kb on - strand, within Ac3H11_138 at 22.471 kb on - strand, within Ac3H11_138 at 22.549 kb on + strand, within Ac3H11_138 at 22.550 kb on - strand, within Ac3H11_138 at 22.614 kb on + strand, within Ac3H11_138 at 22.614 kb on + strand, within Ac3H11_138 at 22.614 kb on + strand, within Ac3H11_138 at 22.615 kb on - strand, within Ac3H11_138 at 22.615 kb on - strand, within Ac3H11_138 at 22.615 kb on - strand, within Ac3H11_138 at 22.615 kb on - strand, within Ac3H11_138 at 22.615 kb on - strand, within Ac3H11_138 at 22.737 kb on + strand, within Ac3H11_138 at 22.737 kb on + strand, within Ac3H11_138 at 22.738 kb on - strand, within Ac3H11_138 at 22.869 kb on + strand, within Ac3H11_138 at 22.869 kb on + strand, within Ac3H11_138 at 22.870 kb on - strand, within Ac3H11_138 at 23.075 kb on + strand at 23.075 kb on + strand at 23.075 kb on + strand at 23.076 kb on - strand at 23.076 kb on - strand at 23.270 kb on + strand, within Ac3H11_139 at 23.300 kb on + strand, within Ac3H11_139 at 23.300 kb on + strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.301 kb on - strand, within Ac3H11_139 at 23.726 kb on + strand, within Ac3H11_139 at 23.727 kb on - strand, within Ac3H11_139
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 21,359 + Ac3H11_137 0.44 +0.2 21,359 + Ac3H11_137 0.44 -1.2 21,659 + Ac3H11_137 0.68 +0.7 21,659 + Ac3H11_137 0.68 -1.3 21,660 - Ac3H11_137 0.68 -0.1 21,660 - Ac3H11_137 0.68 -1.8 21,953 + -0.2 21,954 - +0.7 21,954 - -1.0 21,954 - +3.1 21,954 - -0.1 21,959 + -0.5 21,959 + +0.5 21,959 + -2.6 21,959 + +2.9 21,959 + -3.2 21,959 + -0.2 21,960 - -1.6 21,960 - -0.0 21,960 - +0.3 21,960 - -1.6 21,960 - -0.2 22,073 + -1.4 22,305 + Ac3H11_138 0.21 +0.9 22,305 + Ac3H11_138 0.21 +0.8 22,306 - Ac3H11_138 0.21 -0.1 22,306 - Ac3H11_138 0.21 -0.9 22,306 - Ac3H11_138 0.21 -0.4 22,306 - Ac3H11_138 0.21 +0.5 22,470 + Ac3H11_138 0.39 -0.2 22,470 + Ac3H11_138 0.39 +0.7 22,471 - Ac3H11_138 0.39 +1.7 22,471 - Ac3H11_138 0.39 -0.4 22,549 + Ac3H11_138 0.47 -0.1 22,550 - Ac3H11_138 0.47 -1.3 22,614 + Ac3H11_138 0.54 +0.4 22,614 + Ac3H11_138 0.54 -0.6 22,614 + Ac3H11_138 0.54 +3.1 22,615 - Ac3H11_138 0.54 -2.4 22,615 - Ac3H11_138 0.54 +0.2 22,615 - Ac3H11_138 0.54 +1.1 22,615 - Ac3H11_138 0.54 +0.3 22,615 - Ac3H11_138 0.54 -0.4 22,737 + Ac3H11_138 0.68 +0.9 22,737 + Ac3H11_138 0.68 +0.4 22,738 - Ac3H11_138 0.68 +2.0 22,869 + Ac3H11_138 0.82 +0.1 22,869 + Ac3H11_138 0.82 -0.7 22,870 - Ac3H11_138 0.82 +0.2 23,075 + +0.1 23,075 + -0.6 23,075 + -0.2 23,076 - -1.3 23,076 - -0.6 23,270 + Ac3H11_139 0.24 -0.9 23,300 + Ac3H11_139 0.27 +0.7 23,300 + Ac3H11_139 0.27 -1.0 23,301 - Ac3H11_139 0.27 +0.5 23,301 - Ac3H11_139 0.27 +0.1 23,301 - Ac3H11_139 0.27 +0.1 23,301 - Ac3H11_139 0.27 -0.9 23,301 - Ac3H11_139 0.27 +1.6 23,301 - Ac3H11_139 0.27 -0.8 23,726 + Ac3H11_139 0.75 +1.1 23,727 - Ac3H11_139 0.75 +1.9
Or see this region's nucleotide sequence