Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1026 and Ac3H11_1027 are separated by 269 nucleotides Ac3H11_1027 and Ac3H11_1028 overlap by 4 nucleotides Ac3H11_1028 and Ac3H11_1029 are separated by 10 nucleotides Ac3H11_1029 and Ac3H11_1030 are separated by 86 nucleotides Ac3H11_1030 and Ac3H11_1031 are separated by 261 nucleotides
Ac3H11_1026 - ATP-dependent RNA helicase RhlE, at 148,010 to 149,302
_1026
Ac3H11_1027 - FIG01198047: hypothetical protein, at 149,572 to 149,982
_1027
Ac3H11_1028 - Translation initiation factor SUI1-related protein, at 149,979 to 150,341
_1028
Ac3H11_1029 - Putative cytoplasmic protein, at 150,352 to 150,579
_1029
Ac3H11_1030 - hypothetical protein, at 150,666 to 150,872
_1030
Ac3H11_1031 - hypothetical protein, at 151,134 to 151,307
_1031
Position (kb) 149
150
151 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 149.704 kb on + strand, within Ac3H11_1027 at 150.012 kb on + strand at 150.013 kb on - strand at 150.013 kb on - strand at 150.442 kb on + strand, within Ac3H11_1029 at 150.443 kb on - strand, within Ac3H11_1029 at 150.443 kb on - strand, within Ac3H11_1029 at 150.706 kb on + strand, within Ac3H11_1030 at 150.706 kb on + strand, within Ac3H11_1030 at 150.706 kb on + strand, within Ac3H11_1030 at 150.706 kb on + strand, within Ac3H11_1030 at 150.707 kb on - strand, within Ac3H11_1030 at 150.707 kb on - strand, within Ac3H11_1030 at 150.707 kb on - strand, within Ac3H11_1030 at 150.707 kb on - strand, within Ac3H11_1030 at 150.707 kb on - strand, within Ac3H11_1030 at 150.798 kb on + strand, within Ac3H11_1030 at 150.798 kb on + strand, within Ac3H11_1030 at 150.798 kb on + strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.799 kb on - strand, within Ac3H11_1030 at 150.832 kb on + strand, within Ac3H11_1030 at 150.832 kb on + strand, within Ac3H11_1030 at 150.832 kb on + strand, within Ac3H11_1030 at 150.832 kb on + strand, within Ac3H11_1030 at 150.833 kb on - strand, within Ac3H11_1030 at 150.902 kb on + strand at 150.902 kb on + strand at 150.902 kb on + strand at 150.902 kb on + strand at 150.903 kb on - strand at 150.916 kb on + strand at 150.916 kb on + strand at 150.916 kb on + strand at 150.917 kb on - strand at 150.917 kb on - strand at 150.993 kb on + strand at 150.993 kb on + strand at 151.093 kb on + strand at 151.093 kb on + strand at 151.093 kb on + strand at 151.093 kb on + strand at 151.094 kb on - strand at 151.106 kb on + strand at 151.107 kb on - strand at 151.107 kb on - strand at 151.107 kb on - strand at 151.129 kb on + strand at 151.129 kb on + strand at 151.129 kb on + strand at 151.129 kb on + strand at 151.130 kb on - strand at 151.130 kb on - strand at 151.130 kb on - strand at 151.130 kb on - strand at 151.146 kb on - strand at 151.305 kb on + strand at 151.305 kb on + strand at 151.305 kb on + strand at 151.306 kb on - strand at 151.310 kb on + strand at 151.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 149,704 + Ac3H11_1027 0.32 -1.2 150,012 + +0.0 150,013 - -1.3 150,013 - -2.1 150,442 + Ac3H11_1029 0.39 -2.3 150,443 - Ac3H11_1029 0.40 +0.5 150,443 - Ac3H11_1029 0.40 +2.5 150,706 + Ac3H11_1030 0.19 -1.3 150,706 + Ac3H11_1030 0.19 -0.5 150,706 + Ac3H11_1030 0.19 +0.7 150,706 + Ac3H11_1030 0.19 -0.9 150,707 - Ac3H11_1030 0.20 -1.4 150,707 - Ac3H11_1030 0.20 -0.2 150,707 - Ac3H11_1030 0.20 +0.0 150,707 - Ac3H11_1030 0.20 -2.9 150,707 - Ac3H11_1030 0.20 -1.8 150,798 + Ac3H11_1030 0.64 +0.6 150,798 + Ac3H11_1030 0.64 -0.2 150,798 + Ac3H11_1030 0.64 -0.2 150,799 - Ac3H11_1030 0.64 +1.6 150,799 - Ac3H11_1030 0.64 +0.5 150,799 - Ac3H11_1030 0.64 -0.1 150,799 - Ac3H11_1030 0.64 -1.8 150,799 - Ac3H11_1030 0.64 -0.6 150,799 - Ac3H11_1030 0.64 -0.3 150,799 - Ac3H11_1030 0.64 -2.2 150,799 - Ac3H11_1030 0.64 -0.4 150,799 - Ac3H11_1030 0.64 -1.7 150,799 - Ac3H11_1030 0.64 +2.1 150,832 + Ac3H11_1030 0.80 +0.4 150,832 + Ac3H11_1030 0.80 -1.6 150,832 + Ac3H11_1030 0.80 +0.2 150,832 + Ac3H11_1030 0.80 -0.6 150,833 - Ac3H11_1030 0.81 +1.2 150,902 + -1.4 150,902 + +0.6 150,902 + +0.4 150,902 + +0.6 150,903 - -2.8 150,916 + -0.1 150,916 + -1.7 150,916 + +0.6 150,917 - -0.7 150,917 - -0.2 150,993 + +2.7 150,993 + -1.0 151,093 + +1.7 151,093 + -0.3 151,093 + -1.7 151,093 + +1.5 151,094 - -0.1 151,106 + +0.2 151,107 - -2.0 151,107 - -0.8 151,107 - -0.0 151,129 + -1.2 151,129 + +0.1 151,129 + -2.4 151,129 + -0.0 151,130 - +2.0 151,130 - -0.2 151,130 - -0.8 151,130 - +1.2 151,146 - -0.1 151,305 + +1.8 151,305 + +1.4 151,305 + -1.1 151,306 - -0.9 151,310 + +0.6 151,311 - +0.6
Or see this region's nucleotide sequence