Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1001 and Ac3H11_1002 are separated by 295 nucleotides Ac3H11_1002 and Ac3H11_1003 are separated by 136 nucleotides
Ac3H11_1001 - GGDEF/PAS/PAC-domain containing protein, at 124,548 to 125,720
_1001
Ac3H11_1002 - ABC transporter ATP-binding protein, at 126,016 to 127,086
_1002
Ac3H11_1003 - ABC transporter substrate-binding protein, at 127,223 to 128,461
_1003
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 125.161 kb on + strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.162 kb on - strand, within Ac3H11_1001 at 125.173 kb on + strand, within Ac3H11_1001 at 125.173 kb on + strand, within Ac3H11_1001 at 125.173 kb on + strand, within Ac3H11_1001 at 125.174 kb on - strand, within Ac3H11_1001 at 125.174 kb on - strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.344 kb on + strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.345 kb on - strand, within Ac3H11_1001 at 125.389 kb on + strand, within Ac3H11_1001 at 125.390 kb on - strand, within Ac3H11_1001 at 125.404 kb on + strand, within Ac3H11_1001 at 125.404 kb on + strand, within Ac3H11_1001 at 125.404 kb on + strand, within Ac3H11_1001 at 125.404 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.500 kb on + strand, within Ac3H11_1001 at 125.501 kb on - strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.503 kb on + strand, within Ac3H11_1001 at 125.504 kb on - strand, within Ac3H11_1001 at 125.504 kb on - strand, within Ac3H11_1001 at 125.504 kb on - strand, within Ac3H11_1001 at 125.793 kb on - strand at 125.807 kb on + strand at 125.807 kb on + strand at 125.808 kb on - strand at 125.808 kb on - strand at 125.809 kb on + strand at 125.810 kb on - strand at 125.810 kb on - strand at 125.967 kb on - strand at 126.079 kb on + strand at 126.079 kb on + strand at 126.079 kb on + strand at 126.080 kb on - strand at 126.136 kb on + strand, within Ac3H11_1002 at 126.136 kb on + strand, within Ac3H11_1002 at 126.136 kb on + strand, within Ac3H11_1002 at 126.137 kb on + strand, within Ac3H11_1002 at 126.137 kb on - strand, within Ac3H11_1002 at 126.709 kb on + strand, within Ac3H11_1002 at 126.710 kb on - strand, within Ac3H11_1002 at 126.756 kb on + strand, within Ac3H11_1002 at 126.756 kb on + strand, within Ac3H11_1002 at 126.881 kb on - strand, within Ac3H11_1002 at 126.881 kb on - strand, within Ac3H11_1002 at 126.881 kb on - strand, within Ac3H11_1002 at 126.892 kb on + strand, within Ac3H11_1002 at 126.892 kb on + strand, within Ac3H11_1002 at 126.893 kb on - strand, within Ac3H11_1002 at 126.893 kb on - strand, within Ac3H11_1002 at 126.893 kb on - strand, within Ac3H11_1002 at 126.893 kb on - strand, within Ac3H11_1002 at 126.893 kb on - strand, within Ac3H11_1002 at 126.952 kb on + strand, within Ac3H11_1002 at 127.391 kb on + strand, within Ac3H11_1003 at 127.391 kb on + strand, within Ac3H11_1003 at 127.391 kb on + strand, within Ac3H11_1003 at 127.392 kb on - strand, within Ac3H11_1003 at 127.392 kb on - strand, within Ac3H11_1003 at 127.392 kb on - strand, within Ac3H11_1003 at 127.469 kb on + strand, within Ac3H11_1003 at 127.469 kb on + strand, within Ac3H11_1003 at 127.469 kb on + strand, within Ac3H11_1003 at 127.470 kb on - strand, within Ac3H11_1003 at 127.625 kb on + strand, within Ac3H11_1003 at 127.625 kb on + strand, within Ac3H11_1003 at 127.626 kb on - strand, within Ac3H11_1003 at 127.697 kb on + strand, within Ac3H11_1003 at 127.808 kb on - strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.859 kb on + strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.860 kb on - strand, within Ac3H11_1003 at 127.865 kb on + strand, within Ac3H11_1003 at 127.865 kb on + strand, within Ac3H11_1003
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 125,161 + Ac3H11_1001 0.52 -0.5 125,162 - Ac3H11_1001 0.52 +0.3 125,162 - Ac3H11_1001 0.52 +2.5 125,162 - Ac3H11_1001 0.52 +0.5 125,162 - Ac3H11_1001 0.52 +0.3 125,162 - Ac3H11_1001 0.52 -2.3 125,162 - Ac3H11_1001 0.52 -0.8 125,173 + Ac3H11_1001 0.53 +0.2 125,173 + Ac3H11_1001 0.53 -0.4 125,173 + Ac3H11_1001 0.53 +1.3 125,174 - Ac3H11_1001 0.53 -0.1 125,174 - Ac3H11_1001 0.53 -1.0 125,344 + Ac3H11_1001 0.68 -1.8 125,344 + Ac3H11_1001 0.68 +0.6 125,344 + Ac3H11_1001 0.68 -1.2 125,344 + Ac3H11_1001 0.68 -0.8 125,344 + Ac3H11_1001 0.68 +1.7 125,344 + Ac3H11_1001 0.68 +0.8 125,344 + Ac3H11_1001 0.68 +0.5 125,344 + Ac3H11_1001 0.68 +0.3 125,345 - Ac3H11_1001 0.68 -1.2 125,345 - Ac3H11_1001 0.68 +0.8 125,345 - Ac3H11_1001 0.68 +0.6 125,345 - Ac3H11_1001 0.68 -0.1 125,345 - Ac3H11_1001 0.68 +1.3 125,345 - Ac3H11_1001 0.68 +0.0 125,345 - Ac3H11_1001 0.68 -1.7 125,345 - Ac3H11_1001 0.68 -2.5 125,345 - Ac3H11_1001 0.68 +2.5 125,389 + Ac3H11_1001 0.72 +0.6 125,390 - Ac3H11_1001 0.72 -1.6 125,404 + Ac3H11_1001 0.73 +0.8 125,404 + Ac3H11_1001 0.73 -0.4 125,404 + Ac3H11_1001 0.73 +0.2 125,404 + Ac3H11_1001 0.73 -0.8 125,500 + Ac3H11_1001 0.81 +0.8 125,500 + Ac3H11_1001 0.81 +1.9 125,500 + Ac3H11_1001 0.81 +2.9 125,500 + Ac3H11_1001 0.81 +3.1 125,500 + Ac3H11_1001 0.81 +0.9 125,500 + Ac3H11_1001 0.81 +1.8 125,500 + Ac3H11_1001 0.81 +0.3 125,501 - Ac3H11_1001 0.81 +0.4 125,503 + Ac3H11_1001 0.81 +0.5 125,503 + Ac3H11_1001 0.81 +1.3 125,503 + Ac3H11_1001 0.81 +0.8 125,503 + Ac3H11_1001 0.81 -0.0 125,503 + Ac3H11_1001 0.81 -0.3 125,503 + Ac3H11_1001 0.81 -0.2 125,503 + Ac3H11_1001 0.81 +2.3 125,504 - Ac3H11_1001 0.82 +0.9 125,504 - Ac3H11_1001 0.82 -1.0 125,504 - Ac3H11_1001 0.82 -1.3 125,793 - -1.7 125,807 + -1.1 125,807 + -0.6 125,808 - +0.7 125,808 - -1.0 125,809 + -1.1 125,810 - +0.2 125,810 - -0.8 125,967 - +0.1 126,079 + +3.9 126,079 + +0.2 126,079 + +1.7 126,080 - +1.0 126,136 + Ac3H11_1002 0.11 -1.6 126,136 + Ac3H11_1002 0.11 -0.1 126,136 + Ac3H11_1002 0.11 +1.5 126,137 + Ac3H11_1002 0.11 +4.5 126,137 - Ac3H11_1002 0.11 +1.1 126,709 + Ac3H11_1002 0.65 +1.5 126,710 - Ac3H11_1002 0.65 +0.1 126,756 + Ac3H11_1002 0.69 +0.4 126,756 + Ac3H11_1002 0.69 +0.3 126,881 - Ac3H11_1002 0.81 -1.1 126,881 - Ac3H11_1002 0.81 -1.5 126,881 - Ac3H11_1002 0.81 +0.7 126,892 + Ac3H11_1002 0.82 +0.3 126,892 + Ac3H11_1002 0.82 -1.4 126,893 - Ac3H11_1002 0.82 -0.8 126,893 - Ac3H11_1002 0.82 -1.5 126,893 - Ac3H11_1002 0.82 -1.5 126,893 - Ac3H11_1002 0.82 -0.9 126,893 - Ac3H11_1002 0.82 +0.5 126,952 + Ac3H11_1002 0.87 -1.1 127,391 + Ac3H11_1003 0.14 +0.5 127,391 + Ac3H11_1003 0.14 +0.8 127,391 + Ac3H11_1003 0.14 -0.3 127,392 - Ac3H11_1003 0.14 -0.3 127,392 - Ac3H11_1003 0.14 -0.1 127,392 - Ac3H11_1003 0.14 +0.9 127,469 + Ac3H11_1003 0.20 -1.0 127,469 + Ac3H11_1003 0.20 -0.6 127,469 + Ac3H11_1003 0.20 -0.9 127,470 - Ac3H11_1003 0.20 -0.9 127,625 + Ac3H11_1003 0.32 -1.0 127,625 + Ac3H11_1003 0.32 -2.1 127,626 - Ac3H11_1003 0.33 -0.5 127,697 + Ac3H11_1003 0.38 -0.9 127,808 - Ac3H11_1003 0.47 +0.2 127,859 + Ac3H11_1003 0.51 +2.1 127,859 + Ac3H11_1003 0.51 -1.2 127,859 + Ac3H11_1003 0.51 -0.0 127,859 + Ac3H11_1003 0.51 -1.3 127,859 + Ac3H11_1003 0.51 +1.3 127,859 + Ac3H11_1003 0.51 -1.3 127,860 - Ac3H11_1003 0.51 -1.8 127,860 - Ac3H11_1003 0.51 -1.8 127,860 - Ac3H11_1003 0.51 -1.0 127,860 - Ac3H11_1003 0.51 -0.3 127,865 + Ac3H11_1003 0.52 +0.0 127,865 + Ac3H11_1003 0.52 +1.1
Or see this region's nucleotide sequence