Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1771 and Shew_1772 are separated by 10 nucleotides Shew_1772 and Shew_1773 are separated by 100 nucleotides Shew_1773 and Shew_1774 are separated by 301 nucleotides Shew_1774 and Shew_1775 are separated by 173 nucleotides
Shew_1771: Shew_1771 - nitrate reductase cytochrome c-type subunit (NapB) (RefSeq), at 2,026,726 to 2,027,205
_1771
Shew_1772: Shew_1772 - NapC/NirT family periplasmic nitrate (or nitrite) reductase c-type cytochrome (RefSeq), at 2,027,216 to 2,027,803
_1772
Shew_1773: Shew_1773 - LysR family transcriptional regulator (RefSeq), at 2,027,904 to 2,028,824
_1773
Shew_1774: Shew_1774 - excinuclease ATPase subunit (RefSeq), at 2,029,126 to 2,029,623
_1774
Shew_1775: Shew_1775 - acetolactate synthase 3 regulatory subunit (RefSeq), at 2,029,797 to 2,030,291
_1775
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-1
0
1 at 2027.029 kb on + strand, within Shew_1771 at 2027.150 kb on + strand, within Shew_1771 at 2027.157 kb on - strand, within Shew_1771 at 2027.246 kb on + strand at 2027.902 kb on + strand at 2027.910 kb on - strand at 2027.969 kb on + strand at 2028.088 kb on - strand, within Shew_1773 at 2028.634 kb on + strand, within Shew_1773 at 2028.962 kb on + strand at 2029.290 kb on + strand, within Shew_1774 at 2029.298 kb on - strand, within Shew_1774
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,027,029 + Shew_1771 0.63 -0.1 2,027,150 + Shew_1771 0.88 +0.3 2,027,157 - Shew_1771 0.90 +0.4 2,027,246 + -1.4 2,027,902 + -0.2 2,027,910 - +0.2 2,027,969 + +0.5 2,028,088 - Shew_1773 0.20 -1.1 2,028,634 + Shew_1773 0.79 -0.4 2,028,962 + -0.3 2,029,290 + Shew_1774 0.33 +0.5 2,029,298 - Shew_1774 0.35 +0.2
Or see this region's nucleotide sequence