Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1671 and Shew_1672 are separated by 35 nucleotides Shew_1672 and fabG are separated by 4 nucleotides fabG and Shew_1674 are separated by 113 nucleotides Shew_1674 and Shew_1675 are separated by 9 nucleotides
Shew_1671: Shew_1671 - enoyl-CoA hydratase/isomerase (RefSeq), at 1,920,880 to 1,922,010
_1671
Shew_1672: Shew_1672 - 3-hydroxyisobutyrate dehydrogenase (RefSeq), at 1,922,046 to 1,922,945
_1672
Shew_1673: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 1,922,950 to 1,923,708
fabG
Shew_1674: Shew_1674 - hypothetical protein (RefSeq), at 1,923,822 to 1,924,079
_1674
Shew_1675: Shew_1675 - peptidase S9 prolyl oligopeptidase (RefSeq), at 1,924,089 to 1,926,140
_1675
Position (kb)
1922
1923
1924 Strain fitness (log2 ratio)
-1
0
1 at 1922.151 kb on + strand, within Shew_1672 at 1922.159 kb on - strand, within Shew_1672 at 1922.386 kb on - strand, within Shew_1672 at 1922.386 kb on - strand, within Shew_1672 at 1922.492 kb on + strand, within Shew_1672 at 1922.492 kb on + strand, within Shew_1672 at 1922.500 kb on - strand, within Shew_1672 at 1922.500 kb on - strand, within Shew_1672 at 1922.683 kb on - strand, within Shew_1672 at 1922.989 kb on - strand at 1923.385 kb on - strand, within fabG at 1923.400 kb on - strand, within fabG at 1924.267 kb on - strand at 1924.585 kb on - strand, within Shew_1675
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,922,151 + Shew_1672 0.12 -0.1 1,922,159 - Shew_1672 0.13 +0.0 1,922,386 - Shew_1672 0.38 -1.3 1,922,386 - Shew_1672 0.38 +0.1 1,922,492 + Shew_1672 0.50 +0.1 1,922,492 + Shew_1672 0.50 +0.5 1,922,500 - Shew_1672 0.50 +0.3 1,922,500 - Shew_1672 0.50 +0.1 1,922,683 - Shew_1672 0.71 +0.5 1,922,989 - +0.8 1,923,385 - fabG Shew_1673 0.57 -1.1 1,923,400 - fabG Shew_1673 0.59 -0.7 1,924,267 - +0.1 1,924,585 - Shew_1675 0.24 +0.1
Or see this region's nucleotide sequence