Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_72 and Ac3H11_73 are separated by 215 nucleotides Ac3H11_73 and Ac3H11_74 are separated by 27 nucleotides Ac3H11_74 and Ac3H11_75 are separated by 111 nucleotides Ac3H11_75 and Ac3H11_76 are separated by 8 nucleotides
Ac3H11_72 - D-(-)-3-hydroxybutyrate oligomer hydrolase, at 76,316 to 77,191
_72
Ac3H11_73 - Phenylalanyl-tRNA synthetase beta subunit, at 77,407 to 77,874
_73
Ac3H11_74 - hypothetical protein, at 77,902 to 78,144
_74
Ac3H11_75 - Outer membrane lipoprotein blc precursor, at 78,256 to 78,822
_75
Ac3H11_76 - COG3380: Amine oxidase, flavin-containing, at 78,831 to 79,835
_76
Position (kb) 77
78
79 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 76.965 kb on - strand, within Ac3H11_72 at 76.983 kb on - strand, within Ac3H11_72 at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.267 kb on + strand at 77.268 kb on - strand at 77.367 kb on + strand at 77.473 kb on + strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.678 kb on - strand, within Ac3H11_73 at 77.866 kb on + strand at 77.866 kb on + strand at 77.867 kb on - strand at 77.867 kb on - strand at 77.872 kb on + strand at 77.873 kb on - strand at 78.012 kb on - strand, within Ac3H11_74 at 78.012 kb on - strand, within Ac3H11_74 at 78.123 kb on - strand at 78.155 kb on + strand at 78.255 kb on + strand at 78.466 kb on + strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.613 kb on + strand, within Ac3H11_75 at 78.622 kb on + strand, within Ac3H11_75 at 78.649 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.830 kb on + strand at 78.830 kb on + strand at 78.830 kb on + strand at 78.831 kb on - strand at 78.831 kb on - strand at 79.014 kb on + strand, within Ac3H11_76
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 76,965 - Ac3H11_72 0.74 +0.3 76,983 - Ac3H11_72 0.76 -0.3 77,241 - +0.6 77,241 - -2.0 77,241 - +3.5 77,241 - -1.1 77,267 + -1.7 77,268 - -0.6 77,367 + -2.5 77,473 + Ac3H11_73 0.14 +1.4 77,474 - Ac3H11_73 0.14 +0.2 77,474 - Ac3H11_73 0.14 -0.4 77,474 - Ac3H11_73 0.14 +0.5 77,474 - Ac3H11_73 0.14 -0.9 77,668 + Ac3H11_73 0.56 +0.5 77,668 + Ac3H11_73 0.56 -2.7 77,678 - Ac3H11_73 0.58 +0.4 77,866 + -0.7 77,866 + -0.9 77,867 - +1.0 77,867 - +0.6 77,872 + +1.7 77,873 - -0.7 78,012 - Ac3H11_74 0.45 -0.6 78,012 - Ac3H11_74 0.45 +2.2 78,123 - +0.3 78,155 + +1.6 78,255 + +0.0 78,466 + Ac3H11_75 0.37 -0.0 78,467 - Ac3H11_75 0.37 +0.7 78,467 - Ac3H11_75 0.37 -0.6 78,613 + Ac3H11_75 0.63 +0.8 78,622 + Ac3H11_75 0.65 -0.3 78,649 + Ac3H11_75 0.69 -0.5 78,685 + Ac3H11_75 0.76 -0.3 78,685 + Ac3H11_75 0.76 -0.3 78,685 + Ac3H11_75 0.76 -0.3 78,686 - Ac3H11_75 0.76 +0.5 78,686 - Ac3H11_75 0.76 -0.8 78,830 + +2.2 78,830 + +0.6 78,830 + -0.1 78,831 - +1.4 78,831 - -0.2 79,014 + Ac3H11_76 0.18 +0.2
Or see this region's nucleotide sequence