Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4899 and Ac3H11_4900 overlap by 17 nucleotides Ac3H11_4900 and Ac3H11_4901 are separated by 6 nucleotides Ac3H11_4901 and Ac3H11_4902 are separated by 160 nucleotides Ac3H11_4902 and Ac3H11_4903 overlap by 4 nucleotides
Ac3H11_4899 - ABC transporter ATP-binding protein, at 65,076 to 65,870
_4899
Ac3H11_4900 - Putative amino acid ABC transporter, permease protein, at 65,854 to 66,507
_4900
Ac3H11_4901 - amino acid ABC transporter, permease protein, at 66,514 to 67,188
_4901
Ac3H11_4902 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 67,349 to 68,170
_4902
Ac3H11_4903 - Transcriptional regulator, GntR family, at 68,167 to 68,961
_4903
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 65.774 kb on - strand, within Ac3H11_4899 at 65.774 kb on - strand, within Ac3H11_4899 at 65.774 kb on - strand, within Ac3H11_4899 at 65.774 kb on - strand, within Ac3H11_4899 at 65.853 kb on - strand at 65.853 kb on - strand at 65.927 kb on + strand, within Ac3H11_4900 at 65.927 kb on + strand, within Ac3H11_4900 at 65.948 kb on + strand, within Ac3H11_4900 at 65.948 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.966 kb on + strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 65.967 kb on - strand, within Ac3H11_4900 at 66.291 kb on - strand, within Ac3H11_4900 at 66.337 kb on + strand, within Ac3H11_4900 at 66.337 kb on + strand, within Ac3H11_4900 at 66.337 kb on + strand at 66.337 kb on + strand, within Ac3H11_4900 at 66.337 kb on + strand, within Ac3H11_4900 at 66.337 kb on + strand, within Ac3H11_4900 at 66.337 kb on + strand, within Ac3H11_4900 at 66.338 kb on - strand, within Ac3H11_4900 at 66.338 kb on - strand, within Ac3H11_4900 at 66.338 kb on - strand, within Ac3H11_4900 at 66.338 kb on - strand, within Ac3H11_4900 at 66.341 kb on + strand, within Ac3H11_4900 at 66.342 kb on - strand, within Ac3H11_4900 at 66.406 kb on + strand, within Ac3H11_4900 at 66.406 kb on + strand, within Ac3H11_4900 at 66.515 kb on + strand at 66.515 kb on + strand at 66.515 kb on + strand at 66.515 kb on + strand at 66.584 kb on + strand, within Ac3H11_4901 at 66.584 kb on + strand, within Ac3H11_4901 at 66.584 kb on + strand, within Ac3H11_4901 at 66.584 kb on + strand, within Ac3H11_4901 at 66.653 kb on + strand, within Ac3H11_4901 at 66.653 kb on + strand, within Ac3H11_4901 at 66.654 kb on - strand, within Ac3H11_4901 at 66.654 kb on - strand, within Ac3H11_4901 at 66.867 kb on - strand, within Ac3H11_4901 at 66.998 kb on + strand, within Ac3H11_4901 at 66.998 kb on + strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 66.999 kb on - strand, within Ac3H11_4901 at 67.026 kb on + strand, within Ac3H11_4901 at 67.515 kb on + strand, within Ac3H11_4902 at 67.515 kb on + strand, within Ac3H11_4902 at 67.515 kb on + strand, within Ac3H11_4902 at 67.516 kb on - strand, within Ac3H11_4902 at 67.516 kb on - strand, within Ac3H11_4902 at 67.516 kb on - strand, within Ac3H11_4902 at 67.785 kb on + strand, within Ac3H11_4902 at 67.785 kb on + strand, within Ac3H11_4902 at 67.873 kb on - strand, within Ac3H11_4902 at 67.873 kb on - strand, within Ac3H11_4902 at 67.873 kb on - strand, within Ac3H11_4902 at 67.938 kb on + strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 67.939 kb on - strand, within Ac3H11_4902 at 68.171 kb on - strand at 68.171 kb on - strand at 68.183 kb on - strand at 68.183 kb on - strand at 68.183 kb on - strand at 68.186 kb on + strand at 68.186 kb on + strand at 68.187 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 65,774 - Ac3H11_4899 0.88 +0.8 65,774 - Ac3H11_4899 0.88 -0.2 65,774 - Ac3H11_4899 0.88 -2.0 65,774 - Ac3H11_4899 0.88 +0.6 65,853 - +0.6 65,853 - +0.5 65,927 + Ac3H11_4900 0.11 +1.0 65,927 + Ac3H11_4900 0.11 -1.1 65,948 + Ac3H11_4900 0.14 -1.1 65,948 + Ac3H11_4900 0.14 -0.2 65,966 + Ac3H11_4900 0.17 +0.7 65,966 + Ac3H11_4900 0.17 -1.4 65,966 + Ac3H11_4900 0.17 -0.7 65,966 + Ac3H11_4900 0.17 -1.6 65,966 + Ac3H11_4900 0.17 -0.6 65,966 + Ac3H11_4900 0.17 -1.2 65,967 - Ac3H11_4900 0.17 -0.8 65,967 - Ac3H11_4900 0.17 -0.4 65,967 - Ac3H11_4900 0.17 +0.0 65,967 - Ac3H11_4900 0.17 +1.4 65,967 - Ac3H11_4900 0.17 +0.3 65,967 - Ac3H11_4900 0.17 +0.5 66,291 - Ac3H11_4900 0.67 -0.4 66,337 + Ac3H11_4900 0.74 +0.6 66,337 + Ac3H11_4900 0.74 +2.0 66,337 + +2.6 66,337 + Ac3H11_4900 0.74 -0.6 66,337 + Ac3H11_4900 0.74 +0.6 66,337 + Ac3H11_4900 0.74 +0.5 66,337 + Ac3H11_4900 0.74 -2.0 66,338 - Ac3H11_4900 0.74 +0.4 66,338 - Ac3H11_4900 0.74 +1.6 66,338 - Ac3H11_4900 0.74 -0.6 66,338 - Ac3H11_4900 0.74 -1.2 66,341 + Ac3H11_4900 0.74 +0.3 66,342 - Ac3H11_4900 0.75 +0.6 66,406 + Ac3H11_4900 0.84 -0.3 66,406 + Ac3H11_4900 0.84 +0.1 66,515 + -1.1 66,515 + -0.5 66,515 + +0.8 66,515 + +4.8 66,584 + Ac3H11_4901 0.10 -1.6 66,584 + Ac3H11_4901 0.10 -0.9 66,584 + Ac3H11_4901 0.10 -0.0 66,584 + Ac3H11_4901 0.10 +0.9 66,653 + Ac3H11_4901 0.21 -0.4 66,653 + Ac3H11_4901 0.21 -3.0 66,654 - Ac3H11_4901 0.21 +1.1 66,654 - Ac3H11_4901 0.21 +1.0 66,867 - Ac3H11_4901 0.52 -0.8 66,998 + Ac3H11_4901 0.72 +1.2 66,998 + Ac3H11_4901 0.72 +2.5 66,999 - Ac3H11_4901 0.72 -0.4 66,999 - Ac3H11_4901 0.72 -1.7 66,999 - Ac3H11_4901 0.72 -2.9 66,999 - Ac3H11_4901 0.72 -0.4 66,999 - Ac3H11_4901 0.72 +0.4 66,999 - Ac3H11_4901 0.72 +1.6 67,026 + Ac3H11_4901 0.76 -1.8 67,515 + Ac3H11_4902 0.20 -0.5 67,515 + Ac3H11_4902 0.20 -0.8 67,515 + Ac3H11_4902 0.20 -0.8 67,516 - Ac3H11_4902 0.20 -1.0 67,516 - Ac3H11_4902 0.20 +2.1 67,516 - Ac3H11_4902 0.20 -1.0 67,785 + Ac3H11_4902 0.53 +1.6 67,785 + Ac3H11_4902 0.53 -1.4 67,873 - Ac3H11_4902 0.64 +0.3 67,873 - Ac3H11_4902 0.64 -1.1 67,873 - Ac3H11_4902 0.64 -0.6 67,938 + Ac3H11_4902 0.72 +1.6 67,939 - Ac3H11_4902 0.72 +0.2 67,939 - Ac3H11_4902 0.72 -2.2 67,939 - Ac3H11_4902 0.72 +0.4 67,939 - Ac3H11_4902 0.72 -0.8 67,939 - Ac3H11_4902 0.72 -0.1 67,939 - Ac3H11_4902 0.72 +1.6 68,171 - +0.7 68,171 - -0.0 68,183 - +0.5 68,183 - +0.8 68,183 - +1.3 68,186 + -1.3 68,186 + -1.8 68,187 - +0.8
Or see this region's nucleotide sequence