Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4653 and Ac3H11_4654 are separated by 3 nucleotides Ac3H11_4654 and Ac3H11_4655 are separated by 45 nucleotides Ac3H11_4655 and Ac3H11_4656 are separated by 3 nucleotides Ac3H11_4656 and Ac3H11_4657 are separated by 162 nucleotides
Ac3H11_4653 - Mll3930 protein, at 261,744 to 262,130
_4653
Ac3H11_4654 - Organic hydroperoxide resistance protein, at 262,134 to 262,577
_4654
Ac3H11_4655 - Organic hydroperoxide resistance transcriptional regulator, at 262,623 to 263,087
_4655
Ac3H11_4656 - hypothetical protein, at 263,091 to 263,219
_4656
Ac3H11_4657 - Enoyl-CoA hydratase (EC 4.2.1.17), at 263,382 to 264,200
_4657
Position (kb) 263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 262.135 kb on + strand at 262.135 kb on + strand at 262.136 kb on - strand at 262.136 kb on - strand at 262.136 kb on - strand at 262.136 kb on - strand at 262.136 kb on - strand at 262.136 kb on - strand at 262.183 kb on + strand, within Ac3H11_4654 at 262.183 kb on + strand, within Ac3H11_4654 at 262.184 kb on - strand, within Ac3H11_4654 at 262.276 kb on + strand, within Ac3H11_4654 at 262.525 kb on + strand, within Ac3H11_4654 at 262.525 kb on + strand, within Ac3H11_4654 at 262.526 kb on - strand, within Ac3H11_4654 at 262.939 kb on + strand, within Ac3H11_4655 at 262.939 kb on + strand, within Ac3H11_4655 at 262.939 kb on + strand, within Ac3H11_4655 at 262.940 kb on - strand, within Ac3H11_4655 at 262.940 kb on - strand, within Ac3H11_4655 at 262.940 kb on - strand, within Ac3H11_4655 at 262.940 kb on - strand, within Ac3H11_4655 at 262.948 kb on + strand, within Ac3H11_4655 at 262.948 kb on + strand, within Ac3H11_4655 at 262.948 kb on + strand, within Ac3H11_4655 at 262.981 kb on + strand, within Ac3H11_4655 at 262.981 kb on + strand, within Ac3H11_4655 at 262.981 kb on + strand, within Ac3H11_4655 at 262.981 kb on + strand, within Ac3H11_4655 at 262.981 kb on + strand, within Ac3H11_4655 at 262.982 kb on - strand, within Ac3H11_4655 at 262.982 kb on - strand, within Ac3H11_4655 at 263.011 kb on + strand, within Ac3H11_4655 at 263.012 kb on - strand, within Ac3H11_4655 at 263.194 kb on + strand, within Ac3H11_4656 at 263.194 kb on + strand, within Ac3H11_4656 at 263.195 kb on - strand, within Ac3H11_4656 at 263.195 kb on - strand, within Ac3H11_4656 at 263.195 kb on - strand, within Ac3H11_4656 at 263.195 kb on - strand, within Ac3H11_4656 at 263.208 kb on + strand at 263.208 kb on + strand at 263.208 kb on + strand at 263.208 kb on + strand at 263.208 kb on + strand at 263.209 kb on - strand at 263.209 kb on - strand at 263.209 kb on - strand at 263.209 kb on - strand at 263.209 kb on - strand at 263.209 kb on - strand at 263.217 kb on + strand at 263.217 kb on + strand at 263.217 kb on + strand at 263.218 kb on - strand at 263.218 kb on - strand at 263.218 kb on - strand at 263.218 kb on - strand at 263.261 kb on - strand at 263.261 kb on - strand at 263.275 kb on - strand at 263.903 kb on - strand, within Ac3H11_4657 at 263.956 kb on + strand, within Ac3H11_4657 at 264.055 kb on + strand, within Ac3H11_4657 at 264.055 kb on + strand, within Ac3H11_4657 at 264.055 kb on + strand, within Ac3H11_4657 at 264.055 kb on + strand, within Ac3H11_4657 at 264.056 kb on - strand, within Ac3H11_4657 at 264.056 kb on - strand, within Ac3H11_4657 at 264.056 kb on - strand, within Ac3H11_4657 at 264.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 262,135 + +2.0 262,135 + +0.5 262,136 - -2.2 262,136 - -0.2 262,136 - -1.9 262,136 - +1.4 262,136 - +2.0 262,136 - -0.3 262,183 + Ac3H11_4654 0.11 -0.3 262,183 + Ac3H11_4654 0.11 -1.7 262,184 - Ac3H11_4654 0.11 -0.6 262,276 + Ac3H11_4654 0.32 -0.7 262,525 + Ac3H11_4654 0.88 +0.2 262,525 + Ac3H11_4654 0.88 -2.2 262,526 - Ac3H11_4654 0.88 +0.9 262,939 + Ac3H11_4655 0.68 -1.2 262,939 + Ac3H11_4655 0.68 +1.0 262,939 + Ac3H11_4655 0.68 +0.3 262,940 - Ac3H11_4655 0.68 +0.2 262,940 - Ac3H11_4655 0.68 +4.1 262,940 - Ac3H11_4655 0.68 +0.6 262,940 - Ac3H11_4655 0.68 +0.8 262,948 + Ac3H11_4655 0.70 +1.1 262,948 + Ac3H11_4655 0.70 -0.3 262,948 + Ac3H11_4655 0.70 +0.3 262,981 + Ac3H11_4655 0.77 -0.7 262,981 + Ac3H11_4655 0.77 +0.8 262,981 + Ac3H11_4655 0.77 -1.8 262,981 + Ac3H11_4655 0.77 +1.6 262,981 + Ac3H11_4655 0.77 -0.3 262,982 - Ac3H11_4655 0.77 +0.2 262,982 - Ac3H11_4655 0.77 -1.2 263,011 + Ac3H11_4655 0.83 -2.0 263,012 - Ac3H11_4655 0.84 +0.5 263,194 + Ac3H11_4656 0.80 +4.2 263,194 + Ac3H11_4656 0.80 +0.1 263,195 - Ac3H11_4656 0.81 +0.3 263,195 - Ac3H11_4656 0.81 -0.0 263,195 - Ac3H11_4656 0.81 -3.1 263,195 - Ac3H11_4656 0.81 +0.7 263,208 + +0.4 263,208 + +1.0 263,208 + +1.2 263,208 + -0.7 263,208 + +0.4 263,209 - +0.3 263,209 - +0.5 263,209 - -0.7 263,209 - -2.9 263,209 - -1.0 263,209 - +0.3 263,217 + +0.0 263,217 + -2.5 263,217 + +2.1 263,218 - -1.5 263,218 - -1.6 263,218 - -0.1 263,218 - +1.7 263,261 - -2.5 263,261 - +0.5 263,275 - +0.6 263,903 - Ac3H11_4657 0.64 -2.3 263,956 + Ac3H11_4657 0.70 -1.7 264,055 + Ac3H11_4657 0.82 +1.7 264,055 + Ac3H11_4657 0.82 +2.9 264,055 + Ac3H11_4657 0.82 -1.2 264,055 + Ac3H11_4657 0.82 -1.7 264,056 - Ac3H11_4657 0.82 +0.4 264,056 - Ac3H11_4657 0.82 -1.3 264,056 - Ac3H11_4657 0.82 -0.1 264,119 + +0.3
Or see this region's nucleotide sequence