Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_46

Experiment: inner cut, R2A soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_44 and Ac3H11_45 overlap by 30 nucleotidesAc3H11_45 and Ac3H11_46 are separated by 127 nucleotidesAc3H11_46 and Ac3H11_47 are separated by 47 nucleotidesAc3H11_47 and Ac3H11_48 are separated by 99 nucleotides Ac3H11_44 - TPR repeat precursor, at 41,082 to 42,158 _44 Ac3H11_45 - hypothetical protein, at 42,129 to 42,263 _45 Ac3H11_46 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 42,391 to 43,764 _46 Ac3H11_47 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 43,812 to 44,486 _47 Ac3H11_48 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 44,586 to 45,563 _48 Position (kb) 42 43 44Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 41.471 kb on + strand, within Ac3H11_44at 41.472 kb on - strand, within Ac3H11_44at 41.719 kb on + strand, within Ac3H11_44at 41.720 kb on - strand, within Ac3H11_44at 41.720 kb on - strand, within Ac3H11_44at 41.720 kb on - strand, within Ac3H11_44at 41.720 kb on - strand, within Ac3H11_44at 41.720 kb on - strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.743 kb on + strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.744 kb on - strand, within Ac3H11_44at 41.776 kb on + strand, within Ac3H11_44at 41.776 kb on + strand, within Ac3H11_44at 41.776 kb on + strand, within Ac3H11_44at 41.845 kb on + strand, within Ac3H11_44at 41.846 kb on - strand, within Ac3H11_44at 41.866 kb on + strand, within Ac3H11_44at 41.866 kb on + strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.867 kb on - strand, within Ac3H11_44at 41.887 kb on + strand, within Ac3H11_44at 42.068 kb on - strandat 42.068 kb on - strandat 42.068 kb on - strandat 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.203 kb on + strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.204 kb on - strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.205 kb on + strand, within Ac3H11_45at 42.220 kb on + strand, within Ac3H11_45at 42.220 kb on + strand, within Ac3H11_45at 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.272 kb on + strandat 42.329 kb on + strandat 42.329 kb on + strandat 43.824 kb on - strandat 43.982 kb on + strand, within Ac3H11_47at 44.151 kb on + strand, within Ac3H11_47at 44.152 kb on - strand, within Ac3H11_47at 44.295 kb on + strand, within Ac3H11_47at 44.314 kb on + strand, within Ac3H11_47at 44.361 kb on + strand, within Ac3H11_47at 44.361 kb on + strand, within Ac3H11_47at 44.445 kb on + strandat 44.481 kb on + strandat 44.481 kb on + strandat 44.481 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay
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41,471 + Ac3H11_44 0.36 +0.1
41,472 - Ac3H11_44 0.36 -0.2
41,719 + Ac3H11_44 0.59 -0.3
41,720 - Ac3H11_44 0.59 -0.9
41,720 - Ac3H11_44 0.59 -2.1
41,720 - Ac3H11_44 0.59 +0.0
41,720 - Ac3H11_44 0.59 -1.5
41,720 - Ac3H11_44 0.59 -0.6
41,743 + Ac3H11_44 0.61 -1.1
41,743 + Ac3H11_44 0.61 -0.7
41,743 + Ac3H11_44 0.61 +0.4
41,743 + Ac3H11_44 0.61 -0.5
41,743 + Ac3H11_44 0.61 -0.0
41,743 + Ac3H11_44 0.61 -0.6
41,744 - Ac3H11_44 0.61 -1.2
41,744 - Ac3H11_44 0.61 -1.2
41,744 - Ac3H11_44 0.61 -0.2
41,744 - Ac3H11_44 0.61 -2.3
41,744 - Ac3H11_44 0.61 -0.8
41,744 - Ac3H11_44 0.61 -1.9
41,744 - Ac3H11_44 0.61 -0.5
41,744 - Ac3H11_44 0.61 -1.0
41,776 + Ac3H11_44 0.64 -0.3
41,776 + Ac3H11_44 0.64 +0.8
41,776 + Ac3H11_44 0.64 -1.3
41,845 + Ac3H11_44 0.71 -0.1
41,846 - Ac3H11_44 0.71 -0.5
41,866 + Ac3H11_44 0.73 -0.1
41,866 + Ac3H11_44 0.73 +1.0
41,867 - Ac3H11_44 0.73 +0.7
41,867 - Ac3H11_44 0.73 -0.8
41,867 - Ac3H11_44 0.73 +0.2
41,867 - Ac3H11_44 0.73 -1.7
41,867 - Ac3H11_44 0.73 -1.0
41,867 - Ac3H11_44 0.73 -1.0
41,887 + Ac3H11_44 0.75 -0.2
42,068 - -0.3
42,068 - -0.8
42,068 - -1.3
42,203 + Ac3H11_45 0.55 +0.2
42,203 + Ac3H11_45 0.55 +0.1
42,203 + Ac3H11_45 0.55 +0.3
42,203 + Ac3H11_45 0.55 +0.5
42,203 + Ac3H11_45 0.55 -3.8
42,203 + Ac3H11_45 0.55 -3.1
42,203 + Ac3H11_45 0.55 +0.1
42,203 + Ac3H11_45 0.55 -1.5
42,203 + Ac3H11_45 0.55 -1.0
42,203 + Ac3H11_45 0.55 -1.8
42,203 + Ac3H11_45 0.55 +0.7
42,203 + Ac3H11_45 0.55 -3.7
42,203 + Ac3H11_45 0.55 -0.1
42,203 + Ac3H11_45 0.55 +1.6
42,204 - Ac3H11_45 0.56 -1.5
42,204 - Ac3H11_45 0.56 -0.6
42,204 - Ac3H11_45 0.56 +1.5
42,204 - Ac3H11_45 0.56 +0.3
42,204 - Ac3H11_45 0.56 -0.1
42,204 - Ac3H11_45 0.56 -0.4
42,204 - Ac3H11_45 0.56 +0.7
42,204 - Ac3H11_45 0.56 -1.0
42,204 - Ac3H11_45 0.56 -0.5
42,204 - Ac3H11_45 0.56 -0.3
42,204 - Ac3H11_45 0.56 -1.4
42,204 - Ac3H11_45 0.56 +0.1
42,204 - Ac3H11_45 0.56 +0.2
42,204 - Ac3H11_45 0.56 -0.2
42,204 - Ac3H11_45 0.56 -1.0
42,205 + Ac3H11_45 0.56 +0.2
42,205 + Ac3H11_45 0.56 -1.5
42,205 + Ac3H11_45 0.56 +0.1
42,205 + Ac3H11_45 0.56 -0.4
42,205 + Ac3H11_45 0.56 -0.1
42,205 + Ac3H11_45 0.56 -2.1
42,205 + Ac3H11_45 0.56 -1.1
42,205 + Ac3H11_45 0.56 -1.6
42,220 + Ac3H11_45 0.67 -2.0
42,220 + Ac3H11_45 0.67 -0.0
42,272 + -0.8
42,272 + +0.8
42,272 + -0.3
42,272 + +0.3
42,272 + -0.7
42,272 + +0.4
42,272 + +0.8
42,272 + +0.8
42,272 + +0.7
42,272 + -0.5
42,329 + -0.5
42,329 + -3.1
43,824 - +0.1
43,982 + Ac3H11_47 0.25 -2.7
44,151 + Ac3H11_47 0.50 -1.6
44,152 - Ac3H11_47 0.50 -0.3
44,295 + Ac3H11_47 0.72 -0.5
44,314 + Ac3H11_47 0.74 -1.2
44,361 + Ac3H11_47 0.81 -1.3
44,361 + Ac3H11_47 0.81 -0.3
44,445 + -0.6
44,481 + -1.6
44,481 + +0.9
44,481 + -1.5

Or see this region's nucleotide sequence