Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3954 and Ac3H11_3955 are separated by 6 nucleotides Ac3H11_3955 and Ac3H11_3956 are separated by 2 nucleotides Ac3H11_3956 and Ac3H11_3957 are separated by 88 nucleotides
Ac3H11_3954 - hypothetical protein, not 6-phosphogluconolactonase, at 178,708 to 179,739
_3954
Ac3H11_3955 - TRAP-type C4-dicarboxylate transport system, large permease component, at 179,746 to 181,029
_3955
Ac3H11_3956 - TRAP-type C4-dicarboxylate transport system, small permease component, at 181,032 to 181,550
_3956
Ac3H11_3957 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 181,639 to 182,628
_3957
Position (kb) 179
180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 178.899 kb on + strand, within Ac3H11_3954 at 178.900 kb on - strand, within Ac3H11_3954 at 178.985 kb on + strand, within Ac3H11_3954 at 178.986 kb on - strand, within Ac3H11_3954 at 179.351 kb on + strand, within Ac3H11_3954 at 179.351 kb on + strand, within Ac3H11_3954 at 179.352 kb on - strand, within Ac3H11_3954 at 179.352 kb on - strand, within Ac3H11_3954 at 179.352 kb on - strand, within Ac3H11_3954 at 179.352 kb on - strand, within Ac3H11_3954 at 179.579 kb on + strand, within Ac3H11_3954 at 179.580 kb on - strand, within Ac3H11_3954 at 179.580 kb on - strand, within Ac3H11_3954 at 179.683 kb on + strand at 179.683 kb on + strand at 179.683 kb on + strand at 179.683 kb on + strand at 179.684 kb on - strand at 179.684 kb on - strand at 179.720 kb on - strand at 179.720 kb on - strand at 179.792 kb on + strand at 179.792 kb on + strand at 179.792 kb on + strand at 179.793 kb on - strand at 179.793 kb on - strand at 179.793 kb on - strand at 179.793 kb on - strand at 180.089 kb on + strand, within Ac3H11_3955 at 180.089 kb on + strand, within Ac3H11_3955 at 180.089 kb on + strand, within Ac3H11_3955 at 180.089 kb on + strand, within Ac3H11_3955 at 180.089 kb on + strand, within Ac3H11_3955 at 180.158 kb on + strand, within Ac3H11_3955 at 180.159 kb on - strand, within Ac3H11_3955 at 180.251 kb on + strand, within Ac3H11_3955 at 180.261 kb on - strand, within Ac3H11_3955 at 180.558 kb on - strand, within Ac3H11_3955 at 180.966 kb on - strand at 181.016 kb on - strand at 181.023 kb on + strand at 181.023 kb on + strand at 181.024 kb on - strand at 181.150 kb on + strand, within Ac3H11_3956 at 181.150 kb on + strand, within Ac3H11_3956 at 181.150 kb on + strand, within Ac3H11_3956 at 181.150 kb on + strand, within Ac3H11_3956 at 181.151 kb on - strand, within Ac3H11_3956 at 181.151 kb on - strand, within Ac3H11_3956 at 181.151 kb on - strand, within Ac3H11_3956 at 181.151 kb on - strand, within Ac3H11_3956 at 181.514 kb on - strand at 181.550 kb on + strand at 181.550 kb on + strand at 181.551 kb on - strand at 181.551 kb on - strand at 181.640 kb on + strand at 181.640 kb on + strand at 181.640 kb on + strand at 181.640 kb on + strand at 181.640 kb on + strand at 181.640 kb on + strand at 181.641 kb on - strand at 181.641 kb on - strand at 181.676 kb on + strand at 181.676 kb on + strand at 181.676 kb on + strand at 181.677 kb on - strand at 181.718 kb on + strand at 181.718 kb on + strand at 181.719 kb on - strand at 181.778 kb on + strand, within Ac3H11_3957 at 181.778 kb on + strand, within Ac3H11_3957 at 181.778 kb on + strand, within Ac3H11_3957 at 181.934 kb on + strand, within Ac3H11_3957 at 181.934 kb on + strand, within Ac3H11_3957
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 178,899 + Ac3H11_3954 0.19 -2.7 178,900 - Ac3H11_3954 0.19 +0.4 178,985 + Ac3H11_3954 0.27 -0.8 178,986 - Ac3H11_3954 0.27 -0.4 179,351 + Ac3H11_3954 0.62 -0.6 179,351 + Ac3H11_3954 0.62 -1.9 179,352 - Ac3H11_3954 0.62 -1.0 179,352 - Ac3H11_3954 0.62 -1.6 179,352 - Ac3H11_3954 0.62 -0.3 179,352 - Ac3H11_3954 0.62 -0.8 179,579 + Ac3H11_3954 0.84 +1.9 179,580 - Ac3H11_3954 0.84 +3.0 179,580 - Ac3H11_3954 0.84 -0.9 179,683 + +1.4 179,683 + -1.5 179,683 + -1.0 179,683 + -0.5 179,684 - +1.1 179,684 - +0.3 179,720 - -0.6 179,720 - -1.5 179,792 + +0.5 179,792 + +0.5 179,792 + +1.5 179,793 - +0.0 179,793 - +0.6 179,793 - -0.5 179,793 - +1.4 180,089 + Ac3H11_3955 0.27 -2.9 180,089 + Ac3H11_3955 0.27 +0.5 180,089 + Ac3H11_3955 0.27 +0.2 180,089 + Ac3H11_3955 0.27 -3.3 180,089 + Ac3H11_3955 0.27 -2.0 180,158 + Ac3H11_3955 0.32 +0.8 180,159 - Ac3H11_3955 0.32 +0.3 180,251 + Ac3H11_3955 0.39 +2.0 180,261 - Ac3H11_3955 0.40 +0.8 180,558 - Ac3H11_3955 0.63 -0.6 180,966 - -0.8 181,016 - -0.8 181,023 + -1.5 181,023 + +0.5 181,024 - -0.0 181,150 + Ac3H11_3956 0.23 -0.1 181,150 + Ac3H11_3956 0.23 -1.2 181,150 + Ac3H11_3956 0.23 -0.8 181,150 + Ac3H11_3956 0.23 -2.9 181,151 - Ac3H11_3956 0.23 +0.3 181,151 - Ac3H11_3956 0.23 -0.8 181,151 - Ac3H11_3956 0.23 +0.1 181,151 - Ac3H11_3956 0.23 +0.1 181,514 - -0.7 181,550 + +1.4 181,550 + +0.0 181,551 - +0.0 181,551 - -2.1 181,640 + +0.5 181,640 + +0.3 181,640 + -1.6 181,640 + -0.4 181,640 + +0.8 181,640 + -2.5 181,641 - -1.8 181,641 - -1.5 181,676 + +0.6 181,676 + +0.4 181,676 + +1.0 181,677 - -0.5 181,718 + +0.8 181,718 + -0.7 181,719 - -0.5 181,778 + Ac3H11_3957 0.14 +0.2 181,778 + Ac3H11_3957 0.14 +0.7 181,778 + Ac3H11_3957 0.14 +0.8 181,934 + Ac3H11_3957 0.30 -2.2 181,934 + Ac3H11_3957 0.30 -0.2
Or see this region's nucleotide sequence