Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_379 and Ac3H11_380 are separated by 244 nucleotides Ac3H11_380 and Ac3H11_381 are separated by 521 nucleotides Ac3H11_381 and Ac3H11_566 are separated by 178 nucleotides Ac3H11_566 and Ac3H11_382 are separated by 194 nucleotides
Ac3H11_379 - hypothetical protein, at 56,409 to 56,615
_379
Ac3H11_380 - hypothetical protein, at 56,860 to 57,036
_380
Ac3H11_381 - Integrase, at 57,558 to 58,805
_381
Ac3H11_566 - tRNA-Arg-TCT, at 58,984 to 59,057
_566
Ac3H11_382 - TrkA-N:Sodium/hydrogen exchanger, at 59,252 to 60,991
_382
Position (kb) 57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 56.616 kb on + strand at 56.616 kb on + strand at 56.616 kb on + strand at 56.616 kb on + strand at 56.616 kb on + strand at 56.616 kb on + strand at 56.616 kb on + strand at 56.617 kb on - strand at 56.617 kb on - strand at 56.746 kb on + strand at 56.785 kb on + strand at 56.786 kb on - strand at 56.795 kb on - strand at 56.795 kb on - strand at 56.825 kb on + strand at 56.825 kb on + strand at 56.826 kb on - strand at 56.826 kb on - strand at 56.826 kb on - strand at 56.862 kb on - strand at 56.897 kb on + strand, within Ac3H11_380 at 56.897 kb on + strand, within Ac3H11_380 at 56.898 kb on - strand, within Ac3H11_380 at 56.898 kb on - strand, within Ac3H11_380 at 56.905 kb on + strand, within Ac3H11_380 at 56.906 kb on - strand, within Ac3H11_380 at 56.993 kb on + strand, within Ac3H11_380 at 57.049 kb on + strand at 57.049 kb on + strand at 57.050 kb on - strand at 57.050 kb on - strand at 57.050 kb on - strand at 57.103 kb on + strand at 57.103 kb on + strand at 57.103 kb on + strand at 57.144 kb on + strand at 57.144 kb on + strand at 57.145 kb on - strand at 57.145 kb on - strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.148 kb on + strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.149 kb on - strand at 57.170 kb on + strand at 57.171 kb on - strand at 57.195 kb on - strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.274 kb on - strand at 57.274 kb on - strand at 57.613 kb on + strand at 57.614 kb on - strand at 57.614 kb on - strand at 57.670 kb on + strand at 57.670 kb on + strand at 57.671 kb on - strand at 57.671 kb on - strand at 57.757 kb on - strand, within Ac3H11_381 at 58.018 kb on - strand, within Ac3H11_381 at 58.018 kb on - strand, within Ac3H11_381 at 58.132 kb on + strand, within Ac3H11_381 at 58.132 kb on + strand, within Ac3H11_381 at 58.132 kb on + strand, within Ac3H11_381 at 58.132 kb on + strand, within Ac3H11_381 at 58.133 kb on - strand, within Ac3H11_381 at 58.133 kb on - strand, within Ac3H11_381 at 58.149 kb on + strand, within Ac3H11_381 at 58.150 kb on - strand, within Ac3H11_381 at 58.205 kb on + strand, within Ac3H11_381 at 58.206 kb on - strand, within Ac3H11_381 at 58.336 kb on - strand, within Ac3H11_381 at 58.336 kb on - strand, within Ac3H11_381 at 58.336 kb on - strand, within Ac3H11_381 at 58.399 kb on + strand, within Ac3H11_381 at 58.400 kb on - strand, within Ac3H11_381 at 58.429 kb on - strand, within Ac3H11_381 at 58.429 kb on - strand, within Ac3H11_381 at 58.444 kb on + strand, within Ac3H11_381 at 58.445 kb on - strand, within Ac3H11_381 at 58.518 kb on + strand, within Ac3H11_381 at 58.518 kb on + strand, within Ac3H11_381 at 58.519 kb on - strand, within Ac3H11_381 at 58.519 kb on - strand, within Ac3H11_381 at 58.563 kb on + strand, within Ac3H11_381 at 58.563 kb on + strand, within Ac3H11_381 at 58.563 kb on + strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.564 kb on - strand, within Ac3H11_381 at 58.633 kb on + strand, within Ac3H11_381 at 58.675 kb on + strand, within Ac3H11_381 at 58.675 kb on + strand, within Ac3H11_381 at 58.676 kb on - strand, within Ac3H11_381 at 58.676 kb on - strand, within Ac3H11_381 at 58.676 kb on - strand, within Ac3H11_381 at 58.676 kb on - strand, within Ac3H11_381 at 58.690 kb on + strand at 58.690 kb on + strand at 58.690 kb on + strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.691 kb on - strand at 58.718 kb on - strand at 58.718 kb on - strand at 58.782 kb on + strand at 58.782 kb on + strand at 58.796 kb on + strand at 58.823 kb on + strand at 58.877 kb on + strand at 58.877 kb on + strand at 58.877 kb on + strand at 58.878 kb on - strand at 58.933 kb on - strand at 58.977 kb on + strand at 58.977 kb on + strand at 58.978 kb on - strand at 59.081 kb on - strand at 59.197 kb on - strand at 59.198 kb on + strand at 59.198 kb on + strand at 59.198 kb on + strand at 59.198 kb on + strand at 59.199 kb on - strand at 59.199 kb on - strand at 59.357 kb on + strand at 59.560 kb on + strand, within Ac3H11_382 at 59.560 kb on + strand, within Ac3H11_382 at 59.561 kb on - strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.684 kb on + strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382 at 59.685 kb on - strand, within Ac3H11_382
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 56,616 + -0.2 56,616 + +0.1 56,616 + -0.3 56,616 + -1.4 56,616 + +1.3 56,616 + +0.2 56,616 + -1.2 56,617 - -1.0 56,617 - -1.5 56,746 + -0.6 56,785 + -0.4 56,786 - -1.1 56,795 - +1.1 56,795 - +0.6 56,825 + -1.0 56,825 + +1.4 56,826 - +0.5 56,826 - -0.6 56,826 - +1.5 56,862 - -0.8 56,897 + Ac3H11_380 0.21 +0.6 56,897 + Ac3H11_380 0.21 -0.9 56,898 - Ac3H11_380 0.21 +1.7 56,898 - Ac3H11_380 0.21 -0.2 56,905 + Ac3H11_380 0.25 -3.2 56,906 - Ac3H11_380 0.26 +1.6 56,993 + Ac3H11_380 0.75 +0.7 57,049 + +0.3 57,049 + +0.8 57,050 - +0.0 57,050 - +0.7 57,050 - -0.5 57,103 + +0.7 57,103 + -1.2 57,103 + +0.8 57,144 + +1.1 57,144 + -0.3 57,145 - +1.1 57,145 - +0.9 57,148 + +0.7 57,148 + +0.2 57,148 + -0.5 57,148 + -1.8 57,148 + +0.4 57,148 + -0.1 57,148 + -1.7 57,148 + -0.5 57,148 + -1.3 57,148 + -0.9 57,148 + -2.2 57,148 + -1.2 57,149 - +2.9 57,149 - -1.2 57,149 - +0.3 57,149 - +1.0 57,149 - +0.4 57,149 - -1.9 57,149 - -0.1 57,149 - -0.8 57,149 - -0.1 57,149 - +1.8 57,170 + -2.5 57,171 - +1.2 57,195 - +1.9 57,273 + -0.1 57,273 + -1.1 57,273 + -0.1 57,273 + -0.7 57,274 - -1.0 57,274 - +0.0 57,613 + +0.4 57,614 - -0.5 57,614 - +1.0 57,670 + -1.9 57,670 + -1.2 57,671 - +0.1 57,671 - +0.3 57,757 - Ac3H11_381 0.16 -0.8 58,018 - Ac3H11_381 0.37 -0.9 58,018 - Ac3H11_381 0.37 +0.0 58,132 + Ac3H11_381 0.46 -0.1 58,132 + Ac3H11_381 0.46 -0.1 58,132 + Ac3H11_381 0.46 -0.1 58,132 + Ac3H11_381 0.46 -0.3 58,133 - Ac3H11_381 0.46 -1.3 58,133 - Ac3H11_381 0.46 +1.1 58,149 + Ac3H11_381 0.47 +0.1 58,150 - Ac3H11_381 0.47 +4.1 58,205 + Ac3H11_381 0.52 +0.7 58,206 - Ac3H11_381 0.52 -0.9 58,336 - Ac3H11_381 0.62 -0.3 58,336 - Ac3H11_381 0.62 -0.0 58,336 - Ac3H11_381 0.62 +1.0 58,399 + Ac3H11_381 0.67 -0.7 58,400 - Ac3H11_381 0.67 +0.1 58,429 - Ac3H11_381 0.70 +1.0 58,429 - Ac3H11_381 0.70 +1.6 58,444 + Ac3H11_381 0.71 +0.7 58,445 - Ac3H11_381 0.71 -0.6 58,518 + Ac3H11_381 0.77 +0.3 58,518 + Ac3H11_381 0.77 -0.4 58,519 - Ac3H11_381 0.77 -2.3 58,519 - Ac3H11_381 0.77 -0.0 58,563 + Ac3H11_381 0.81 +0.3 58,563 + Ac3H11_381 0.81 +1.6 58,563 + Ac3H11_381 0.81 +0.5 58,564 - Ac3H11_381 0.81 -1.8 58,564 - Ac3H11_381 0.81 -0.7 58,564 - Ac3H11_381 0.81 +0.1 58,564 - Ac3H11_381 0.81 +0.7 58,564 - Ac3H11_381 0.81 +0.4 58,564 - Ac3H11_381 0.81 -1.9 58,564 - Ac3H11_381 0.81 +0.4 58,564 - Ac3H11_381 0.81 -1.8 58,633 + Ac3H11_381 0.86 -1.1 58,675 + Ac3H11_381 0.90 +0.7 58,675 + Ac3H11_381 0.90 -0.9 58,676 - Ac3H11_381 0.90 +0.1 58,676 - Ac3H11_381 0.90 +1.1 58,676 - Ac3H11_381 0.90 -0.6 58,676 - Ac3H11_381 0.90 -1.0 58,690 + -0.9 58,690 + +1.2 58,690 + +1.2 58,691 - -0.2 58,691 - +0.2 58,691 - +0.8 58,691 - +0.7 58,691 - +1.8 58,691 - -0.9 58,691 - -0.2 58,718 - +0.1 58,718 - +0.1 58,782 + +1.0 58,782 + +1.8 58,796 + +2.5 58,823 + -0.3 58,877 + -0.7 58,877 + +0.5 58,877 + -0.2 58,878 - -0.4 58,933 - -1.3 58,977 + +1.0 58,977 + +1.1 58,978 - +1.2 59,081 - -2.3 59,197 - +1.0 59,198 + +1.1 59,198 + -1.5 59,198 + +0.2 59,198 + -0.5 59,199 - -0.1 59,199 - +0.2 59,357 + -2.1 59,560 + Ac3H11_382 0.18 -0.8 59,560 + Ac3H11_382 0.18 +1.8 59,561 - Ac3H11_382 0.18 +1.0 59,684 + Ac3H11_382 0.25 -0.4 59,684 + Ac3H11_382 0.25 -0.6 59,684 + Ac3H11_382 0.25 +5.1 59,684 + Ac3H11_382 0.25 -2.0 59,684 + Ac3H11_382 0.25 -0.6 59,684 + Ac3H11_382 0.25 -2.3 59,684 + Ac3H11_382 0.25 -0.2 59,685 - Ac3H11_382 0.25 -0.4 59,685 - Ac3H11_382 0.25 +0.2 59,685 - Ac3H11_382 0.25 +1.5 59,685 - Ac3H11_382 0.25 -0.6 59,685 - Ac3H11_382 0.25 -0.6 59,685 - Ac3H11_382 0.25 -0.7 59,685 - Ac3H11_382 0.25 +0.2
Or see this region's nucleotide sequence