Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3779 and Ac3H11_3780 are separated by 2 nucleotides Ac3H11_3780 and Ac3H11_3781 are separated by 118 nucleotides Ac3H11_3781 and Ac3H11_3782 are separated by 4 nucleotides Ac3H11_3782 and Ac3H11_3783 are separated by 43 nucleotides
Ac3H11_3779 - Mu-like prophage FluMu protein gp29, at 27,056 to 28,627
_3779
Ac3H11_3780 - Phage (Mu-like) virion morphogenesis protein, at 28,630 to 29,907
_3780
Ac3H11_3781 - hypothetical protein, at 30,026 to 30,481
_3781
Ac3H11_3782 - hypothetical protein, at 30,486 to 30,686
_3782
Ac3H11_3783 - Phage protein, at 30,730 to 31,311
_3783
Position (kb) 28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 27.650 kb on + strand, within Ac3H11_3779 at 27.764 kb on + strand, within Ac3H11_3779 at 27.765 kb on - strand, within Ac3H11_3779 at 27.923 kb on + strand, within Ac3H11_3779 at 27.924 kb on - strand, within Ac3H11_3779 at 28.100 kb on + strand, within Ac3H11_3779 at 28.128 kb on - strand, within Ac3H11_3779 at 28.232 kb on + strand, within Ac3H11_3779 at 28.233 kb on - strand, within Ac3H11_3779 at 28.233 kb on - strand, within Ac3H11_3779 at 28.531 kb on - strand at 28.984 kb on + strand, within Ac3H11_3780 at 28.985 kb on - strand, within Ac3H11_3780 at 28.985 kb on - strand, within Ac3H11_3780 at 29.008 kb on + strand, within Ac3H11_3780 at 29.008 kb on + strand, within Ac3H11_3780 at 29.009 kb on - strand, within Ac3H11_3780 at 29.219 kb on - strand, within Ac3H11_3780 at 29.219 kb on - strand, within Ac3H11_3780 at 29.219 kb on - strand, within Ac3H11_3780 at 29.327 kb on + strand, within Ac3H11_3780 at 29.327 kb on + strand, within Ac3H11_3780 at 29.327 kb on + strand, within Ac3H11_3780 at 29.331 kb on + strand, within Ac3H11_3780 at 29.332 kb on - strand, within Ac3H11_3780 at 29.332 kb on - strand, within Ac3H11_3780 at 29.332 kb on - strand, within Ac3H11_3780 at 29.332 kb on - strand, within Ac3H11_3780 at 29.332 kb on - strand, within Ac3H11_3780 at 29.351 kb on - strand, within Ac3H11_3780 at 29.833 kb on + strand at 29.834 kb on - strand at 29.834 kb on - strand at 29.953 kb on + strand at 30.072 kb on - strand, within Ac3H11_3781 at 30.228 kb on - strand, within Ac3H11_3781 at 30.456 kb on + strand at 30.456 kb on + strand at 30.457 kb on - strand at 30.538 kb on - strand, within Ac3H11_3782 at 30.595 kb on - strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.604 kb on - strand, within Ac3H11_3782 at 30.604 kb on - strand, within Ac3H11_3782 at 30.615 kb on + strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.679 kb on - strand at 30.742 kb on + strand at 30.857 kb on + strand, within Ac3H11_3783 at 30.858 kb on - strand, within Ac3H11_3783
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 27,650 + Ac3H11_3779 0.38 +2.5 27,764 + Ac3H11_3779 0.45 +3.3 27,765 - Ac3H11_3779 0.45 -1.0 27,923 + Ac3H11_3779 0.55 -0.3 27,924 - Ac3H11_3779 0.55 -0.3 28,100 + Ac3H11_3779 0.66 -1.2 28,128 - Ac3H11_3779 0.68 -1.4 28,232 + Ac3H11_3779 0.75 +0.2 28,233 - Ac3H11_3779 0.75 +0.6 28,233 - Ac3H11_3779 0.75 -0.6 28,531 - +0.9 28,984 + Ac3H11_3780 0.28 +0.8 28,985 - Ac3H11_3780 0.28 +1.4 28,985 - Ac3H11_3780 0.28 +0.9 29,008 + Ac3H11_3780 0.30 -0.5 29,008 + Ac3H11_3780 0.30 -2.7 29,009 - Ac3H11_3780 0.30 -1.1 29,219 - Ac3H11_3780 0.46 -0.8 29,219 - Ac3H11_3780 0.46 +0.6 29,219 - Ac3H11_3780 0.46 +0.4 29,327 + Ac3H11_3780 0.55 -1.0 29,327 + Ac3H11_3780 0.55 +0.9 29,327 + Ac3H11_3780 0.55 +0.6 29,331 + Ac3H11_3780 0.55 -0.2 29,332 - Ac3H11_3780 0.55 -0.9 29,332 - Ac3H11_3780 0.55 +1.3 29,332 - Ac3H11_3780 0.55 +3.1 29,332 - Ac3H11_3780 0.55 +1.7 29,332 - Ac3H11_3780 0.55 -1.2 29,351 - Ac3H11_3780 0.56 -3.6 29,833 + +0.5 29,834 - -1.2 29,834 - -0.6 29,953 + +0.4 30,072 - Ac3H11_3781 0.10 +1.5 30,228 - Ac3H11_3781 0.44 +0.7 30,456 + -1.4 30,456 + +1.2 30,457 - +1.0 30,538 - Ac3H11_3782 0.26 -0.8 30,595 - Ac3H11_3782 0.54 +0.3 30,603 + Ac3H11_3782 0.58 +3.1 30,603 + Ac3H11_3782 0.58 -2.3 30,603 + Ac3H11_3782 0.58 -1.4 30,604 - Ac3H11_3782 0.59 +0.5 30,604 - Ac3H11_3782 0.59 +0.6 30,615 + Ac3H11_3782 0.64 -2.2 30,616 - Ac3H11_3782 0.65 +0.8 30,616 - Ac3H11_3782 0.65 +0.2 30,616 - Ac3H11_3782 0.65 +0.5 30,616 - Ac3H11_3782 0.65 +0.6 30,616 - Ac3H11_3782 0.65 -1.0 30,616 - Ac3H11_3782 0.65 -1.5 30,679 - +0.9 30,742 + +0.4 30,857 + Ac3H11_3783 0.22 +2.2 30,858 - Ac3H11_3783 0.22 -0.2
Or see this region's nucleotide sequence