Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3736 and Ac3H11_3737 are separated by 70 nucleotides Ac3H11_3737 and Ac3H11_3738 overlap by 4 nucleotides
Ac3H11_3736 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 94,801 to 95,868
_3736
Ac3H11_3737 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 95,939 to 96,649
_3737
Ac3H11_3738 - FIG00641944: hypothetical protein, at 96,646 to 97,923
_3738
Position (kb)
95
96
97 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 95.039 kb on + strand, within Ac3H11_3736 at 95.040 kb on - strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.063 kb on + strand, within Ac3H11_3736 at 95.064 kb on - strand, within Ac3H11_3736 at 95.064 kb on - strand, within Ac3H11_3736 at 95.064 kb on - strand, within Ac3H11_3736 at 95.065 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.231 kb on + strand, within Ac3H11_3736 at 95.232 kb on - strand, within Ac3H11_3736 at 95.480 kb on + strand, within Ac3H11_3736 at 95.480 kb on + strand, within Ac3H11_3736 at 95.480 kb on + strand, within Ac3H11_3736 at 95.481 kb on - strand, within Ac3H11_3736 at 95.481 kb on - strand, within Ac3H11_3736 at 95.481 kb on - strand, within Ac3H11_3736 at 95.481 kb on - strand, within Ac3H11_3736 at 95.486 kb on + strand, within Ac3H11_3736 at 95.703 kb on - strand, within Ac3H11_3736 at 95.703 kb on - strand, within Ac3H11_3736 at 95.787 kb on - strand at 95.805 kb on + strand at 95.805 kb on + strand at 95.805 kb on + strand at 95.805 kb on + strand at 95.805 kb on + strand at 95.805 kb on + strand at 95.806 kb on - strand at 96.240 kb on + strand, within Ac3H11_3737 at 96.240 kb on + strand, within Ac3H11_3737 at 96.387 kb on + strand, within Ac3H11_3737 at 96.407 kb on + strand, within Ac3H11_3737 at 96.407 kb on + strand, within Ac3H11_3737 at 96.408 kb on - strand, within Ac3H11_3737 at 96.408 kb on - strand, within Ac3H11_3737 at 96.516 kb on + strand, within Ac3H11_3737 at 96.517 kb on - strand, within Ac3H11_3737 at 96.564 kb on + strand, within Ac3H11_3737 at 96.564 kb on + strand, within Ac3H11_3737 at 96.564 kb on + strand, within Ac3H11_3737 at 96.564 kb on + strand, within Ac3H11_3737 at 96.565 kb on - strand, within Ac3H11_3737 at 96.565 kb on - strand, within Ac3H11_3737 at 96.565 kb on - strand, within Ac3H11_3737 at 96.641 kb on - strand at 96.983 kb on + strand, within Ac3H11_3738 at 96.983 kb on + strand, within Ac3H11_3738 at 96.983 kb on + strand, within Ac3H11_3738 at 96.984 kb on - strand, within Ac3H11_3738 at 96.984 kb on - strand, within Ac3H11_3738 at 96.984 kb on - strand, within Ac3H11_3738 at 96.984 kb on - strand, within Ac3H11_3738 at 97.237 kb on + strand, within Ac3H11_3738
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 95,039 + Ac3H11_3736 0.22 -0.2 95,040 - Ac3H11_3736 0.22 -3.1 95,063 + Ac3H11_3736 0.25 -0.5 95,063 + Ac3H11_3736 0.25 +0.6 95,063 + Ac3H11_3736 0.25 -0.6 95,063 + Ac3H11_3736 0.25 +0.2 95,063 + Ac3H11_3736 0.25 -1.7 95,063 + Ac3H11_3736 0.25 +0.8 95,063 + Ac3H11_3736 0.25 +0.2 95,064 - Ac3H11_3736 0.25 -2.6 95,064 - Ac3H11_3736 0.25 -1.6 95,064 - Ac3H11_3736 0.25 -2.3 95,065 + Ac3H11_3736 0.25 -0.9 95,231 + Ac3H11_3736 0.40 -0.7 95,231 + Ac3H11_3736 0.40 +1.1 95,231 + Ac3H11_3736 0.40 -1.4 95,231 + Ac3H11_3736 0.40 -1.6 95,231 + Ac3H11_3736 0.40 -0.4 95,231 + Ac3H11_3736 0.40 +0.8 95,231 + Ac3H11_3736 0.40 +1.3 95,231 + Ac3H11_3736 0.40 -1.4 95,232 - Ac3H11_3736 0.40 -1.0 95,480 + Ac3H11_3736 0.64 +0.2 95,480 + Ac3H11_3736 0.64 -0.9 95,480 + Ac3H11_3736 0.64 +1.1 95,481 - Ac3H11_3736 0.64 -1.0 95,481 - Ac3H11_3736 0.64 +0.1 95,481 - Ac3H11_3736 0.64 -0.6 95,481 - Ac3H11_3736 0.64 +0.7 95,486 + Ac3H11_3736 0.64 -0.4 95,703 - Ac3H11_3736 0.84 +0.7 95,703 - Ac3H11_3736 0.84 +1.6 95,787 - -0.9 95,805 + +0.4 95,805 + +1.0 95,805 + +0.0 95,805 + +2.0 95,805 + +2.0 95,805 + -1.4 95,806 - -2.0 96,240 + Ac3H11_3737 0.42 +2.5 96,240 + Ac3H11_3737 0.42 +0.8 96,387 + Ac3H11_3737 0.63 -0.9 96,407 + Ac3H11_3737 0.66 +0.1 96,407 + Ac3H11_3737 0.66 -1.7 96,408 - Ac3H11_3737 0.66 -0.1 96,408 - Ac3H11_3737 0.66 +2.5 96,516 + Ac3H11_3737 0.81 +0.5 96,517 - Ac3H11_3737 0.81 -0.5 96,564 + Ac3H11_3737 0.88 -1.0 96,564 + Ac3H11_3737 0.88 +0.5 96,564 + Ac3H11_3737 0.88 -0.9 96,564 + Ac3H11_3737 0.88 -1.0 96,565 - Ac3H11_3737 0.88 -1.5 96,565 - Ac3H11_3737 0.88 -0.4 96,565 - Ac3H11_3737 0.88 -2.3 96,641 - +0.2 96,983 + Ac3H11_3738 0.26 -0.5 96,983 + Ac3H11_3738 0.26 -2.9 96,983 + Ac3H11_3738 0.26 -0.7 96,984 - Ac3H11_3738 0.26 -0.7 96,984 - Ac3H11_3738 0.26 +2.3 96,984 - Ac3H11_3738 0.26 -0.4 96,984 - Ac3H11_3738 0.26 +1.5 97,237 + Ac3H11_3738 0.46 +1.1
Or see this region's nucleotide sequence