Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3729 and Ac3H11_3730 are separated by 23 nucleotides Ac3H11_3730 and Ac3H11_3731 are separated by 25 nucleotides Ac3H11_3731 and Ac3H11_3732 are separated by 190 nucleotides Ac3H11_3732 and Ac3H11_3733 are separated by 51 nucleotides
Ac3H11_3729 - Cytosine deaminase (EC 3.5.4.1), at 88,802 to 90,130
_3729
Ac3H11_3730 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 90,154 to 90,741
_3730
Ac3H11_3731 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 90,767 to 91,069
_3731
Ac3H11_3732 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 91,260 to 91,934
_3732
Ac3H11_3733 - cytidine/deoxycytidylate deaminase family protein, at 91,986 to 92,561
_3733
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 90.155 kb on + strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.233 kb on + strand, within Ac3H11_3730 at 90.233 kb on + strand, within Ac3H11_3730 at 90.234 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.245 kb on + strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.536 kb on + strand, within Ac3H11_3730 at 90.537 kb on - strand, within Ac3H11_3730 at 90.623 kb on + strand, within Ac3H11_3730 at 90.699 kb on - strand at 90.710 kb on - strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.723 kb on - strand at 90.723 kb on - strand at 91.226 kb on + strand at 91.262 kb on - strand at 91.378 kb on + strand, within Ac3H11_3732 at 91.387 kb on + strand, within Ac3H11_3732 at 91.388 kb on - strand, within Ac3H11_3732 at 91.516 kb on + strand, within Ac3H11_3732 at 91.516 kb on + strand, within Ac3H11_3732 at 91.517 kb on - strand, within Ac3H11_3732 at 91.517 kb on - strand, within Ac3H11_3732 at 91.591 kb on + strand, within Ac3H11_3732 at 91.591 kb on + strand, within Ac3H11_3732 at 91.612 kb on + strand, within Ac3H11_3732 at 91.612 kb on + strand, within Ac3H11_3732 at 91.613 kb on - strand, within Ac3H11_3732 at 91.613 kb on - strand, within Ac3H11_3732 at 91.624 kb on - strand, within Ac3H11_3732 at 91.691 kb on - strand, within Ac3H11_3732 at 91.691 kb on - strand, within Ac3H11_3732 at 91.705 kb on + strand, within Ac3H11_3732 at 91.705 kb on + strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.733 kb on - strand, within Ac3H11_3732 at 91.733 kb on - strand, within Ac3H11_3732
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 90,155 + -0.4 90,156 - -0.5 90,156 - -0.8 90,156 - -0.5 90,156 - +2.7 90,156 - -1.3 90,233 + Ac3H11_3730 0.13 +0.7 90,233 + Ac3H11_3730 0.13 +1.7 90,234 - Ac3H11_3730 0.14 -0.7 90,237 - Ac3H11_3730 0.14 +1.9 90,237 - Ac3H11_3730 0.14 -0.9 90,245 + Ac3H11_3730 0.15 +1.3 90,285 - Ac3H11_3730 0.22 +1.4 90,285 - Ac3H11_3730 0.22 -0.3 90,285 - Ac3H11_3730 0.22 -0.9 90,348 - Ac3H11_3730 0.33 -1.0 90,348 - Ac3H11_3730 0.33 +1.5 90,348 - Ac3H11_3730 0.33 +0.9 90,536 + Ac3H11_3730 0.65 -3.0 90,537 - Ac3H11_3730 0.65 -0.9 90,623 + Ac3H11_3730 0.80 +0.7 90,699 - -1.3 90,710 - +0.3 90,722 + -0.9 90,722 + +2.6 90,722 + +0.9 90,723 - +1.2 90,723 - -0.4 91,226 + -0.3 91,262 - -0.9 91,378 + Ac3H11_3732 0.17 -0.8 91,387 + Ac3H11_3732 0.19 -1.6 91,388 - Ac3H11_3732 0.19 +1.7 91,516 + Ac3H11_3732 0.38 -2.1 91,516 + Ac3H11_3732 0.38 +0.1 91,517 - Ac3H11_3732 0.38 +1.9 91,517 - Ac3H11_3732 0.38 +1.0 91,591 + Ac3H11_3732 0.49 +0.9 91,591 + Ac3H11_3732 0.49 +0.9 91,612 + Ac3H11_3732 0.52 +2.7 91,612 + Ac3H11_3732 0.52 -0.1 91,613 - Ac3H11_3732 0.52 -0.8 91,613 - Ac3H11_3732 0.52 -3.2 91,624 - Ac3H11_3732 0.54 +0.2 91,691 - Ac3H11_3732 0.64 -1.6 91,691 - Ac3H11_3732 0.64 +0.4 91,705 + Ac3H11_3732 0.66 +0.5 91,705 + Ac3H11_3732 0.66 +1.1 91,706 - Ac3H11_3732 0.66 -0.3 91,706 - Ac3H11_3732 0.66 -2.9 91,706 - Ac3H11_3732 0.66 -1.6 91,729 + Ac3H11_3732 0.69 +2.6 91,729 + Ac3H11_3732 0.69 -0.5 91,729 + Ac3H11_3732 0.69 -0.9 91,729 + Ac3H11_3732 0.69 -0.0 91,729 + Ac3H11_3732 0.69 -0.7 91,729 + Ac3H11_3732 0.69 +1.8 91,729 + Ac3H11_3732 0.69 -0.7 91,729 + Ac3H11_3732 0.69 -0.2 91,729 + Ac3H11_3732 0.69 -0.4 91,729 + Ac3H11_3732 0.69 -0.9 91,729 + Ac3H11_3732 0.69 +0.3 91,730 - Ac3H11_3732 0.70 +0.3 91,730 - Ac3H11_3732 0.70 +2.6 91,730 - Ac3H11_3732 0.70 +2.2 91,730 - Ac3H11_3732 0.70 -1.1 91,730 - Ac3H11_3732 0.70 -0.2 91,730 - Ac3H11_3732 0.70 -0.9 91,733 - Ac3H11_3732 0.70 +0.3 91,733 - Ac3H11_3732 0.70 -2.9
Or see this region's nucleotide sequence