Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_3398

Experiment: inner cut, R2A soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_3396 and Ac3H11_3397 are separated by 44 nucleotidesAc3H11_3397 and Ac3H11_3398 are separated by 54 nucleotidesAc3H11_3398 and Ac3H11_3399 are separated by 48 nucleotidesAc3H11_3399 and Ac3H11_3400 are separated by 32 nucleotides Ac3H11_3396 - ProQ: influences osmotic activation of compatible solute ProP, at 73,466 to 74,437 _3396 Ac3H11_3397 - Conserved domain protein, at 74,482 to 75,096 _3397 Ac3H11_3398 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 75,151 to 76,404 _3398 Ac3H11_3399 - Probable transmembrane protein, at 76,453 to 77,250 _3399 Ac3H11_3400 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 77,283 to 78,410 _3400 Position (kb) 75 76 77Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 74.483 kb on + strandat 74.484 kb on - strandat 74.484 kb on - strandat 74.484 kb on - strandat 74.634 kb on - strand, within Ac3H11_3397at 74.634 kb on - strand, within Ac3H11_3397at 74.634 kb on - strand, within Ac3H11_3397at 74.634 kb on - strand, within Ac3H11_3397at 74.756 kb on + strand, within Ac3H11_3397at 74.794 kb on + strand, within Ac3H11_3397at 74.794 kb on + strand, within Ac3H11_3397at 74.795 kb on - strand, within Ac3H11_3397at 74.941 kb on + strand, within Ac3H11_3397at 74.942 kb on - strand, within Ac3H11_3397at 75.133 kb on - strandat 75.320 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.347 kb on + strand, within Ac3H11_3398at 75.348 kb on - strand, within Ac3H11_3398at 75.479 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.485 kb on + strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.486 kb on - strand, within Ac3H11_3398at 75.605 kb on + strand, within Ac3H11_3398at 75.605 kb on + strand, within Ac3H11_3398at 75.635 kb on + strand, within Ac3H11_3398at 76.133 kb on + strand, within Ac3H11_3398at 76.134 kb on - strand, within Ac3H11_3398at 76.134 kb on - strand, within Ac3H11_3398at 76.247 kb on + strand, within Ac3H11_3398at 76.247 kb on + strand, within Ac3H11_3398at 76.247 kb on + strand, within Ac3H11_3398at 76.247 kb on + strand, within Ac3H11_3398at 76.248 kb on - strand, within Ac3H11_3398at 76.248 kb on - strand, within Ac3H11_3398at 76.292 kb on + strandat 76.293 kb on - strandat 76.293 kb on - strandat 76.293 kb on - strandat 76.293 kb on - strandat 77.044 kb on + strand, within Ac3H11_3399at 77.044 kb on + strand, within Ac3H11_3399at 77.044 kb on + strand, within Ac3H11_3399at 77.045 kb on - strand, within Ac3H11_3399at 77.045 kb on - strand, within Ac3H11_3399at 77.045 kb on - strand, within Ac3H11_3399at 77.045 kb on - strand, within Ac3H11_3399at 77.045 kb on - strand, within Ac3H11_3399at 77.126 kb on + strand, within Ac3H11_3399at 77.126 kb on + strand, within Ac3H11_3399at 77.126 kb on + strand, within Ac3H11_3399at 77.126 kb on + strand, within Ac3H11_3399at 77.126 kb on + strand, within Ac3H11_3399at 77.127 kb on - strand, within Ac3H11_3399at 77.127 kb on - strand, within Ac3H11_3399at 77.127 kb on - strand, within Ac3H11_3399at 77.127 kb on - strand, within Ac3H11_3399at 77.127 kb on - strand, within Ac3H11_3399at 77.284 kb on + strandat 77.284 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay
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74,483 + +0.2
74,484 - -1.1
74,484 - -2.7
74,484 - -2.1
74,634 - Ac3H11_3397 0.25 +0.4
74,634 - Ac3H11_3397 0.25 +0.1
74,634 - Ac3H11_3397 0.25 +0.9
74,634 - Ac3H11_3397 0.25 -0.1
74,756 + Ac3H11_3397 0.45 -1.7
74,794 + Ac3H11_3397 0.51 -1.7
74,794 + Ac3H11_3397 0.51 -1.3
74,795 - Ac3H11_3397 0.51 -0.9
74,941 + Ac3H11_3397 0.75 -0.4
74,942 - Ac3H11_3397 0.75 -0.0
75,133 - -0.1
75,320 + Ac3H11_3398 0.13 +1.2
75,347 + Ac3H11_3398 0.16 -0.5
75,347 + Ac3H11_3398 0.16 +2.0
75,347 + Ac3H11_3398 0.16 -1.0
75,347 + Ac3H11_3398 0.16 -1.4
75,347 + Ac3H11_3398 0.16 -0.0
75,347 + Ac3H11_3398 0.16 -0.1
75,348 - Ac3H11_3398 0.16 +0.1
75,479 + Ac3H11_3398 0.26 +1.0
75,485 + Ac3H11_3398 0.27 +0.0
75,485 + Ac3H11_3398 0.27 +0.3
75,485 + Ac3H11_3398 0.27 -3.7
75,485 + Ac3H11_3398 0.27 +0.1
75,485 + Ac3H11_3398 0.27 -2.3
75,485 + Ac3H11_3398 0.27 -0.2
75,485 + Ac3H11_3398 0.27 -2.4
75,485 + Ac3H11_3398 0.27 -1.1
75,485 + Ac3H11_3398 0.27 -1.2
75,485 + Ac3H11_3398 0.27 -1.8
75,486 - Ac3H11_3398 0.27 +0.8
75,486 - Ac3H11_3398 0.27 +0.9
75,486 - Ac3H11_3398 0.27 -0.7
75,486 - Ac3H11_3398 0.27 -1.6
75,486 - Ac3H11_3398 0.27 +0.5
75,486 - Ac3H11_3398 0.27 +0.2
75,486 - Ac3H11_3398 0.27 +1.7
75,486 - Ac3H11_3398 0.27 +1.3
75,486 - Ac3H11_3398 0.27 -3.4
75,486 - Ac3H11_3398 0.27 +0.1
75,486 - Ac3H11_3398 0.27 -0.5
75,486 - Ac3H11_3398 0.27 +0.2
75,486 - Ac3H11_3398 0.27 +0.8
75,605 + Ac3H11_3398 0.36 -0.1
75,605 + Ac3H11_3398 0.36 -1.4
75,635 + Ac3H11_3398 0.39 -0.2
76,133 + Ac3H11_3398 0.78 +0.5
76,134 - Ac3H11_3398 0.78 -0.5
76,134 - Ac3H11_3398 0.78 -0.5
76,247 + Ac3H11_3398 0.87 -0.0
76,247 + Ac3H11_3398 0.87 +0.7
76,247 + Ac3H11_3398 0.87 -2.0
76,247 + Ac3H11_3398 0.87 -0.2
76,248 - Ac3H11_3398 0.87 +0.2
76,248 - Ac3H11_3398 0.87 -2.3
76,292 + -0.9
76,293 - -0.1
76,293 - -1.2
76,293 - -0.6
76,293 - -0.7
77,044 + Ac3H11_3399 0.74 -0.4
77,044 + Ac3H11_3399 0.74 +0.7
77,044 + Ac3H11_3399 0.74 +0.5
77,045 - Ac3H11_3399 0.74 -1.5
77,045 - Ac3H11_3399 0.74 -2.4
77,045 - Ac3H11_3399 0.74 -1.9
77,045 - Ac3H11_3399 0.74 -0.3
77,045 - Ac3H11_3399 0.74 +1.4
77,126 + Ac3H11_3399 0.84 -2.1
77,126 + Ac3H11_3399 0.84 -1.3
77,126 + Ac3H11_3399 0.84 +0.9
77,126 + Ac3H11_3399 0.84 -0.8
77,126 + Ac3H11_3399 0.84 +0.1
77,127 - Ac3H11_3399 0.84 -0.1
77,127 - Ac3H11_3399 0.84 -2.6
77,127 - Ac3H11_3399 0.84 -1.3
77,127 - Ac3H11_3399 0.84 -1.8
77,127 - Ac3H11_3399 0.84 +2.6
77,284 + +0.3
77,284 + +0.8

Or see this region's nucleotide sequence