Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2857 and Ac3H11_2858 are separated by 132 nucleotides Ac3H11_2858 and Ac3H11_2859 are separated by 25 nucleotides Ac3H11_2859 and Ac3H11_2860 are separated by 37 nucleotides Ac3H11_2860 and Ac3H11_2861 are separated by 117 nucleotides
Ac3H11_2857 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 1,163,373 to 1,164,848
_2857
Ac3H11_2858 - Probable transmembrane protein, at 1,164,981 to 1,165,601
_2858
Ac3H11_2859 - UPF0125 protein yfjF, at 1,165,627 to 1,165,974
_2859
Ac3H11_2860 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 1,166,012 to 1,166,452
_2860
Ac3H11_2861 - tmRNA-binding protein SmpB, at 1,166,570 to 1,167,043
_2861
Position (kb) 1164
1165
1166 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1164.740 kb on + strand at 1164.873 kb on + strand at 1164.873 kb on + strand at 1164.873 kb on + strand at 1164.873 kb on + strand at 1164.874 kb on - strand at 1164.874 kb on - strand at 1165.726 kb on + strand, within Ac3H11_2859 at 1165.726 kb on + strand, within Ac3H11_2859 at 1165.726 kb on + strand, within Ac3H11_2859 at 1165.726 kb on + strand, within Ac3H11_2859 at 1165.727 kb on - strand, within Ac3H11_2859 at 1165.742 kb on + strand, within Ac3H11_2859 at 1165.742 kb on + strand, within Ac3H11_2859 at 1165.742 kb on + strand, within Ac3H11_2859 at 1165.743 kb on - strand, within Ac3H11_2859 at 1165.743 kb on - strand, within Ac3H11_2859 at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.019 kb on + strand at 1166.020 kb on - strand at 1166.020 kb on - strand at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.121 kb on + strand, within Ac3H11_2860 at 1166.122 kb on - strand, within Ac3H11_2860 at 1166.122 kb on - strand, within Ac3H11_2860 at 1166.122 kb on - strand, within Ac3H11_2860 at 1166.122 kb on - strand, within Ac3H11_2860 at 1166.122 kb on - strand, within Ac3H11_2860 at 1166.419 kb on - strand at 1166.496 kb on - strand at 1166.496 kb on - strand at 1166.526 kb on + strand at 1166.526 kb on + strand at 1166.526 kb on + strand at 1166.526 kb on + strand at 1166.526 kb on + strand at 1166.527 kb on - strand at 1166.527 kb on - strand at 1166.539 kb on + strand at 1166.539 kb on + strand at 1166.539 kb on + strand at 1166.540 kb on - strand at 1166.540 kb on - strand at 1166.540 kb on - strand at 1166.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 1,164,740 + -1.1 1,164,873 + -1.3 1,164,873 + +1.4 1,164,873 + +0.3 1,164,873 + -2.1 1,164,874 - +0.1 1,164,874 - +0.5 1,165,726 + Ac3H11_2859 0.28 -0.0 1,165,726 + Ac3H11_2859 0.28 +0.4 1,165,726 + Ac3H11_2859 0.28 -2.5 1,165,726 + Ac3H11_2859 0.28 -2.0 1,165,727 - Ac3H11_2859 0.29 -1.0 1,165,742 + Ac3H11_2859 0.33 -2.6 1,165,742 + Ac3H11_2859 0.33 -0.1 1,165,742 + Ac3H11_2859 0.33 +0.5 1,165,743 - Ac3H11_2859 0.33 -0.6 1,165,743 - Ac3H11_2859 0.33 -1.4 1,166,019 + +0.3 1,166,019 + +0.2 1,166,019 + -0.1 1,166,019 + +0.2 1,166,019 + -0.6 1,166,019 + -1.0 1,166,019 + +1.4 1,166,019 + +1.2 1,166,020 - +0.2 1,166,020 - -1.3 1,166,121 + Ac3H11_2860 0.25 -0.5 1,166,121 + Ac3H11_2860 0.25 +0.1 1,166,121 + Ac3H11_2860 0.25 +0.1 1,166,121 + Ac3H11_2860 0.25 -0.3 1,166,121 + Ac3H11_2860 0.25 -1.7 1,166,121 + Ac3H11_2860 0.25 -0.0 1,166,122 - Ac3H11_2860 0.25 +0.1 1,166,122 - Ac3H11_2860 0.25 +0.2 1,166,122 - Ac3H11_2860 0.25 -0.3 1,166,122 - Ac3H11_2860 0.25 -1.2 1,166,122 - Ac3H11_2860 0.25 -1.5 1,166,419 - -1.6 1,166,496 - -0.8 1,166,496 - +0.4 1,166,526 + +1.6 1,166,526 + +1.6 1,166,526 + -0.7 1,166,526 + -1.7 1,166,526 + +0.3 1,166,527 - -1.4 1,166,527 - +3.0 1,166,539 + +0.1 1,166,539 + +1.6 1,166,539 + -1.8 1,166,540 - +0.5 1,166,540 - -0.7 1,166,540 - -1.4 1,166,563 - +1.8
Or see this region's nucleotide sequence