Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2555 and Ac3H11_2556 are separated by 31 nucleotides Ac3H11_2556 and Ac3H11_2557 are separated by 76 nucleotides Ac3H11_2557 and Ac3H11_2558 are separated by 39 nucleotides
Ac3H11_2555 - ABC transporter for L-Histidine, periplasmic substrate-binding component 1 (from data) , at 832,352 to 833,101
_2555
Ac3H11_2556 - Transcriptional regulator, IclR family, at 833,133 to 833,909
_2556
Ac3H11_2557 - Urocanate hydratase (EC 4.2.1.49) (from data) , at 833,986 to 835,710
_2557
Ac3H11_2558 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 835,750 to 837,288
_2558
Position (kb)
833
834
835
836 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 833.392 kb on + strand, within Ac3H11_2556 at 833.392 kb on + strand, within Ac3H11_2556 at 833.392 kb on + strand, within Ac3H11_2556 at 833.392 kb on + strand, within Ac3H11_2556 at 833.743 kb on + strand, within Ac3H11_2556 at 833.889 kb on - strand at 833.897 kb on - strand at 833.897 kb on - strand at 833.897 kb on - strand at 833.914 kb on - strand at 833.965 kb on + strand at 833.987 kb on + strand at 833.987 kb on + strand at 833.988 kb on - strand at 833.988 kb on - strand at 833.988 kb on - strand at 834.222 kb on + strand, within Ac3H11_2557 at 834.222 kb on + strand, within Ac3H11_2557 at 834.611 kb on + strand, within Ac3H11_2557 at 834.611 kb on + strand, within Ac3H11_2557 at 834.612 kb on - strand, within Ac3H11_2557 at 834.612 kb on - strand, within Ac3H11_2557 at 834.612 kb on - strand, within Ac3H11_2557 at 834.612 kb on - strand, within Ac3H11_2557 at 834.612 kb on - strand, within Ac3H11_2557 at 834.689 kb on + strand, within Ac3H11_2557 at 834.689 kb on + strand, within Ac3H11_2557 at 834.689 kb on + strand, within Ac3H11_2557 at 834.843 kb on - strand, within Ac3H11_2557 at 835.043 kb on + strand, within Ac3H11_2557 at 835.043 kb on + strand, within Ac3H11_2557 at 835.043 kb on + strand, within Ac3H11_2557 at 835.044 kb on - strand, within Ac3H11_2557 at 835.044 kb on - strand, within Ac3H11_2557 at 835.193 kb on + strand, within Ac3H11_2557 at 835.226 kb on + strand, within Ac3H11_2557 at 835.226 kb on + strand, within Ac3H11_2557 at 835.226 kb on + strand, within Ac3H11_2557 at 835.226 kb on + strand, within Ac3H11_2557 at 835.227 kb on - strand, within Ac3H11_2557 at 835.227 kb on - strand, within Ac3H11_2557 at 835.227 kb on - strand, within Ac3H11_2557 at 835.259 kb on + strand, within Ac3H11_2557 at 835.259 kb on + strand, within Ac3H11_2557 at 835.260 kb on - strand, within Ac3H11_2557 at 835.260 kb on - strand, within Ac3H11_2557 at 835.289 kb on + strand, within Ac3H11_2557 at 835.289 kb on + strand, within Ac3H11_2557 at 835.289 kb on + strand, within Ac3H11_2557 at 835.290 kb on - strand, within Ac3H11_2557 at 835.290 kb on - strand, within Ac3H11_2557 at 835.290 kb on - strand, within Ac3H11_2557 at 835.481 kb on + strand, within Ac3H11_2557 at 835.481 kb on + strand, within Ac3H11_2557 at 835.481 kb on + strand, within Ac3H11_2557 at 835.481 kb on + strand, within Ac3H11_2557 at 835.482 kb on - strand, within Ac3H11_2557 at 835.482 kb on - strand, within Ac3H11_2557 at 835.482 kb on - strand, within Ac3H11_2557 at 835.482 kb on - strand, within Ac3H11_2557 at 835.520 kb on + strand, within Ac3H11_2557 at 835.520 kb on + strand, within Ac3H11_2557 at 835.521 kb on - strand, within Ac3H11_2557 at 835.562 kb on - strand at 835.562 kb on - strand at 835.562 kb on - strand at 835.842 kb on - strand at 835.842 kb on - strand at 835.842 kb on - strand at 835.859 kb on + strand at 836.015 kb on + strand, within Ac3H11_2558 at 836.015 kb on + strand, within Ac3H11_2558 at 836.016 kb on - strand, within Ac3H11_2558 at 836.016 kb on - strand, within Ac3H11_2558 at 836.340 kb on + strand, within Ac3H11_2558 at 836.438 kb on + strand, within Ac3H11_2558 at 836.439 kb on - strand, within Ac3H11_2558
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 833,392 + Ac3H11_2556 0.33 -1.2 833,392 + Ac3H11_2556 0.33 +0.2 833,392 + Ac3H11_2556 0.33 +0.8 833,392 + Ac3H11_2556 0.33 -0.8 833,743 + Ac3H11_2556 0.79 +0.2 833,889 - +0.6 833,897 - +1.5 833,897 - +0.6 833,897 - -0.1 833,914 - +0.0 833,965 + -0.4 833,987 + -1.3 833,987 + +0.6 833,988 - -0.1 833,988 - +0.1 833,988 - +0.7 834,222 + Ac3H11_2557 0.14 +2.5 834,222 + Ac3H11_2557 0.14 -0.2 834,611 + Ac3H11_2557 0.36 +1.7 834,611 + Ac3H11_2557 0.36 +0.7 834,612 - Ac3H11_2557 0.36 -0.1 834,612 - Ac3H11_2557 0.36 -2.6 834,612 - Ac3H11_2557 0.36 -0.7 834,612 - Ac3H11_2557 0.36 +0.5 834,612 - Ac3H11_2557 0.36 +0.9 834,689 + Ac3H11_2557 0.41 -1.4 834,689 + Ac3H11_2557 0.41 -0.6 834,689 + Ac3H11_2557 0.41 -1.4 834,843 - Ac3H11_2557 0.50 -0.3 835,043 + Ac3H11_2557 0.61 +0.5 835,043 + Ac3H11_2557 0.61 -2.1 835,043 + Ac3H11_2557 0.61 -0.5 835,044 - Ac3H11_2557 0.61 +0.1 835,044 - Ac3H11_2557 0.61 -2.2 835,193 + Ac3H11_2557 0.70 -1.3 835,226 + Ac3H11_2557 0.72 -1.0 835,226 + Ac3H11_2557 0.72 +0.6 835,226 + Ac3H11_2557 0.72 +1.4 835,226 + Ac3H11_2557 0.72 -2.9 835,227 - Ac3H11_2557 0.72 -0.7 835,227 - Ac3H11_2557 0.72 -0.5 835,227 - Ac3H11_2557 0.72 +1.5 835,259 + Ac3H11_2557 0.74 -1.1 835,259 + Ac3H11_2557 0.74 -1.9 835,260 - Ac3H11_2557 0.74 -1.0 835,260 - Ac3H11_2557 0.74 -0.5 835,289 + Ac3H11_2557 0.76 -2.6 835,289 + Ac3H11_2557 0.76 +0.1 835,289 + Ac3H11_2557 0.76 +0.7 835,290 - Ac3H11_2557 0.76 -1.5 835,290 - Ac3H11_2557 0.76 +0.5 835,290 - Ac3H11_2557 0.76 -0.5 835,481 + Ac3H11_2557 0.87 -0.2 835,481 + Ac3H11_2557 0.87 +0.0 835,481 + Ac3H11_2557 0.87 -1.9 835,481 + Ac3H11_2557 0.87 -0.5 835,482 - Ac3H11_2557 0.87 +0.1 835,482 - Ac3H11_2557 0.87 -0.3 835,482 - Ac3H11_2557 0.87 -1.8 835,482 - Ac3H11_2557 0.87 -0.1 835,520 + Ac3H11_2557 0.89 +0.2 835,520 + Ac3H11_2557 0.89 -0.6 835,521 - Ac3H11_2557 0.89 -1.5 835,562 - +2.0 835,562 - -2.2 835,562 - -1.2 835,842 - -2.8 835,842 - -0.0 835,842 - +0.2 835,859 + -1.9 836,015 + Ac3H11_2558 0.17 +2.4 836,015 + Ac3H11_2558 0.17 +0.9 836,016 - Ac3H11_2558 0.17 +0.5 836,016 - Ac3H11_2558 0.17 +0.2 836,340 + Ac3H11_2558 0.38 +1.1 836,438 + Ac3H11_2558 0.45 -0.6 836,439 - Ac3H11_2558 0.45 -2.1
Or see this region's nucleotide sequence