Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2385 and Ac3H11_2386 are separated by 48 nucleotides Ac3H11_2386 and Ac3H11_2387 overlap by 4 nucleotides Ac3H11_2387 and Ac3H11_2388 are separated by 210 nucleotides
Ac3H11_2385 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 644,891 to 646,003
_2385
Ac3H11_2386 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 646,052 to 646,234
_2386
Ac3H11_2387 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 646,231 to 647,049
_2387
Ac3H11_2388 - Adenylate kinase (EC 2.7.4.3), at 647,260 to 647,916
_2388
Position (kb)
646
647
648 Strain fitness (log2 ratio)
-2
-1
0
1 at 646.109 kb on + strand, within Ac3H11_2386 at 646.109 kb on + strand, within Ac3H11_2386 at 647.110 kb on + strand at 647.110 kb on + strand at 647.110 kb on + strand at 647.110 kb on + strand at 647.110 kb on + strand at 647.110 kb on + strand at 647.110 kb on + strand at 647.111 kb on - strand at 647.111 kb on - strand at 647.111 kb on - strand at 647.111 kb on - strand at 647.111 kb on - strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.122 kb on + strand at 647.123 kb on - strand at 647.123 kb on - strand at 647.123 kb on - strand at 647.123 kb on - strand at 647.123 kb on - strand at 647.148 kb on + strand at 647.148 kb on + strand at 647.148 kb on + strand at 647.149 kb on - strand at 647.196 kb on + strand at 647.242 kb on + strand at 647.928 kb on - strand at 647.939 kb on + strand at 647.939 kb on + strand at 647.970 kb on - strand at 648.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 646,109 + Ac3H11_2386 0.31 -0.4 646,109 + Ac3H11_2386 0.31 +0.3 647,110 + +0.0 647,110 + -0.1 647,110 + -0.3 647,110 + +0.9 647,110 + -0.1 647,110 + -0.1 647,110 + -1.2 647,111 - -2.2 647,111 - +0.5 647,111 - -0.1 647,111 - +1.2 647,111 - -2.7 647,122 + +0.2 647,122 + +1.2 647,122 + -0.3 647,122 + +0.8 647,122 + +0.5 647,122 + -1.0 647,122 + -1.1 647,122 + -0.4 647,123 - -0.2 647,123 - -0.5 647,123 - +0.8 647,123 - -0.8 647,123 - -1.1 647,148 + -1.7 647,148 + -1.8 647,148 + -1.5 647,149 - -0.7 647,196 + -2.1 647,242 + -1.2 647,928 - -1.7 647,939 + +0.5 647,939 + +0.5 647,970 - -0.3 648,009 + +0.7
Or see this region's nucleotide sequence