Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2184 and Ac3H11_2185 are separated by 38 nucleotides Ac3H11_2185 and Ac3H11_2186 are separated by 53 nucleotides Ac3H11_2186 and Ac3H11_2187 are separated by 13 nucleotides Ac3H11_2187 and Ac3H11_2188 are separated by 25 nucleotides
Ac3H11_2184 - FIG00348557: hypothetical protein, at 413,901 to 416,738
_2184
Ac3H11_2185 - hypothetical secreted protein, at 416,777 to 417,499
_2185
Ac3H11_2186 - Sensor protein basS/pmrB (EC 2.7.3.-), at 417,553 to 418,854
_2186
Ac3H11_2187 - DNA-binding response regulator, at 418,868 to 419,527
_2187
Ac3H11_2188 - Ni,Fe-hydrogenase I cytochrome b subunit, at 419,553 to 420,098
_2188
Position (kb) 417
418
419 Strain fitness (log2 ratio) -2
-1
0
1
2 at 416.738 kb on + strand at 416.738 kb on + strand at 416.738 kb on + strand at 416.738 kb on + strand at 416.739 kb on - strand at 416.766 kb on + strand at 416.766 kb on + strand at 416.766 kb on + strand at 416.767 kb on - strand at 416.767 kb on - strand at 416.767 kb on - strand at 416.767 kb on - strand at 417.181 kb on + strand, within Ac3H11_2185 at 417.182 kb on - strand, within Ac3H11_2185 at 417.263 kb on + strand, within Ac3H11_2185 at 417.263 kb on + strand, within Ac3H11_2185 at 417.264 kb on - strand, within Ac3H11_2185 at 417.525 kb on - strand at 417.686 kb on + strand, within Ac3H11_2186 at 417.686 kb on + strand, within Ac3H11_2186 at 417.687 kb on - strand, within Ac3H11_2186 at 417.687 kb on - strand, within Ac3H11_2186 at 417.687 kb on - strand, within Ac3H11_2186 at 418.385 kb on + strand, within Ac3H11_2186 at 418.385 kb on + strand, within Ac3H11_2186 at 418.385 kb on + strand, within Ac3H11_2186 at 418.385 kb on + strand, within Ac3H11_2186 at 418.385 kb on + strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.386 kb on - strand, within Ac3H11_2186 at 418.653 kb on - strand, within Ac3H11_2186 at 418.691 kb on - strand, within Ac3H11_2186 at 418.981 kb on + strand, within Ac3H11_2187 at 418.981 kb on + strand, within Ac3H11_2187 at 418.981 kb on + strand, within Ac3H11_2187 at 418.981 kb on + strand, within Ac3H11_2187 at 418.981 kb on + strand, within Ac3H11_2187 at 418.982 kb on - strand, within Ac3H11_2187 at 418.982 kb on - strand, within Ac3H11_2187 at 419.403 kb on + strand, within Ac3H11_2187 at 419.403 kb on + strand, within Ac3H11_2187 at 419.403 kb on + strand, within Ac3H11_2187
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 416,738 + -1.4 416,738 + -2.1 416,738 + -0.9 416,738 + -0.1 416,739 - +1.5 416,766 + +1.0 416,766 + -2.7 416,766 + +1.1 416,767 - +0.8 416,767 - -0.5 416,767 - +1.1 416,767 - +0.9 417,181 + Ac3H11_2185 0.56 -0.2 417,182 - Ac3H11_2185 0.56 -0.1 417,263 + Ac3H11_2185 0.67 +0.7 417,263 + Ac3H11_2185 0.67 +1.5 417,264 - Ac3H11_2185 0.67 +1.9 417,525 - +0.2 417,686 + Ac3H11_2186 0.10 +0.2 417,686 + Ac3H11_2186 0.10 +0.4 417,687 - Ac3H11_2186 0.10 +0.7 417,687 - Ac3H11_2186 0.10 +1.0 417,687 - Ac3H11_2186 0.10 +0.3 418,385 + Ac3H11_2186 0.64 +0.6 418,385 + Ac3H11_2186 0.64 +1.0 418,385 + Ac3H11_2186 0.64 -0.9 418,385 + Ac3H11_2186 0.64 -1.0 418,385 + Ac3H11_2186 0.64 +0.4 418,386 - Ac3H11_2186 0.64 +0.3 418,386 - Ac3H11_2186 0.64 -0.2 418,386 - Ac3H11_2186 0.64 +0.3 418,386 - Ac3H11_2186 0.64 -0.5 418,386 - Ac3H11_2186 0.64 +0.5 418,386 - Ac3H11_2186 0.64 +0.4 418,653 - Ac3H11_2186 0.84 +0.2 418,691 - Ac3H11_2186 0.87 +0.5 418,981 + Ac3H11_2187 0.17 +1.0 418,981 + Ac3H11_2187 0.17 +0.2 418,981 + Ac3H11_2187 0.17 -2.2 418,981 + Ac3H11_2187 0.17 -0.8 418,981 + Ac3H11_2187 0.17 -0.3 418,982 - Ac3H11_2187 0.17 +1.6 418,982 - Ac3H11_2187 0.17 +0.5 419,403 + Ac3H11_2187 0.81 +1.5 419,403 + Ac3H11_2187 0.81 +0.9 419,403 + Ac3H11_2187 0.81 -2.5
Or see this region's nucleotide sequence