Experiment: inner cut, R2A soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_167 and Ac3H11_168 are separated by 115 nucleotides Ac3H11_168 and Ac3H11_169 are separated by 4 nucleotides
Ac3H11_167 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 52,612 to 55,524
_167
Ac3H11_168 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA precursor (EC 5.2.1.8), at 55,640 to 56,044
_168
Ac3H11_169 - Albicidin resistance protein, at 56,049 to 57,101
_169
Position (kb) 55
56
57 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 54.667 kb on + strand, within Ac3H11_167 at 54.668 kb on - strand, within Ac3H11_167 at 54.668 kb on - strand, within Ac3H11_167 at 54.668 kb on - strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.946 kb on + strand, within Ac3H11_167 at 54.947 kb on - strand, within Ac3H11_167 at 54.947 kb on - strand, within Ac3H11_167 at 54.947 kb on - strand, within Ac3H11_167 at 55.012 kb on + strand, within Ac3H11_167 at 55.012 kb on + strand, within Ac3H11_167 at 55.012 kb on + strand, within Ac3H11_167 at 55.012 kb on + strand, within Ac3H11_167 at 55.012 kb on + strand, within Ac3H11_167 at 55.013 kb on - strand, within Ac3H11_167 at 55.013 kb on - strand, within Ac3H11_167 at 55.013 kb on - strand, within Ac3H11_167 at 55.013 kb on - strand, within Ac3H11_167 at 55.013 kb on - strand, within Ac3H11_167 at 55.040 kb on - strand, within Ac3H11_167 at 55.051 kb on + strand, within Ac3H11_167 at 55.051 kb on + strand, within Ac3H11_167 at 55.052 kb on - strand, within Ac3H11_167 at 55.052 kb on - strand, within Ac3H11_167 at 55.387 kb on + strand at 55.387 kb on + strand at 55.408 kb on + strand at 55.438 kb on + strand at 55.438 kb on + strand at 55.438 kb on + strand at 55.439 kb on - strand at 55.439 kb on - strand at 55.516 kb on + strand at 55.516 kb on + strand at 55.517 kb on - strand at 55.599 kb on + strand at 55.627 kb on + strand at 55.628 kb on - strand at 55.736 kb on + strand, within Ac3H11_168 at 55.736 kb on + strand, within Ac3H11_168 at 55.737 kb on - strand, within Ac3H11_168 at 55.737 kb on - strand, within Ac3H11_168 at 55.737 kb on - strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.799 kb on + strand, within Ac3H11_168 at 55.800 kb on - strand, within Ac3H11_168 at 55.800 kb on - strand, within Ac3H11_168 at 55.842 kb on - strand, within Ac3H11_168 at 56.091 kb on - strand at 56.112 kb on + strand at 56.112 kb on + strand at 56.112 kb on + strand at 56.112 kb on + strand at 56.113 kb on - strand at 56.113 kb on - strand at 56.169 kb on + strand, within Ac3H11_169 at 56.169 kb on + strand, within Ac3H11_169 at 56.270 kb on - strand, within Ac3H11_169 at 56.270 kb on - strand, within Ac3H11_169 at 56.270 kb on - strand, within Ac3H11_169 at 56.270 kb on - strand, within Ac3H11_169 at 56.434 kb on - strand, within Ac3H11_169 at 56.434 kb on - strand, within Ac3H11_169 at 56.443 kb on - strand, within Ac3H11_169 at 56.583 kb on + strand, within Ac3H11_169 at 56.716 kb on - strand, within Ac3H11_169 at 57.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, R2A soft agar motility assay remove 54,667 + Ac3H11_167 0.71 -0.6 54,668 - Ac3H11_167 0.71 +2.7 54,668 - Ac3H11_167 0.71 +0.6 54,668 - Ac3H11_167 0.71 -0.9 54,946 + Ac3H11_167 0.80 -1.3 54,946 + Ac3H11_167 0.80 +0.2 54,946 + Ac3H11_167 0.80 -0.9 54,946 + Ac3H11_167 0.80 -2.0 54,946 + Ac3H11_167 0.80 -0.6 54,946 + Ac3H11_167 0.80 -0.5 54,947 - Ac3H11_167 0.80 -0.6 54,947 - Ac3H11_167 0.80 +0.4 54,947 - Ac3H11_167 0.80 -1.0 55,012 + Ac3H11_167 0.82 -0.6 55,012 + Ac3H11_167 0.82 -0.2 55,012 + Ac3H11_167 0.82 +1.1 55,012 + Ac3H11_167 0.82 -1.3 55,012 + Ac3H11_167 0.82 +0.2 55,013 - Ac3H11_167 0.82 -0.3 55,013 - Ac3H11_167 0.82 -1.2 55,013 - Ac3H11_167 0.82 -0.2 55,013 - Ac3H11_167 0.82 -1.0 55,013 - Ac3H11_167 0.82 -1.1 55,040 - Ac3H11_167 0.83 +0.1 55,051 + Ac3H11_167 0.84 -1.0 55,051 + Ac3H11_167 0.84 +0.8 55,052 - Ac3H11_167 0.84 -2.8 55,052 - Ac3H11_167 0.84 +0.7 55,387 + +0.6 55,387 + +0.8 55,408 + +1.1 55,438 + -1.9 55,438 + +0.4 55,438 + -0.1 55,439 - -1.2 55,439 - -1.7 55,516 + +1.3 55,516 + -1.2 55,517 - +0.7 55,599 + -0.2 55,627 + +0.3 55,628 - -0.3 55,736 + Ac3H11_168 0.24 -1.0 55,736 + Ac3H11_168 0.24 +0.2 55,737 - Ac3H11_168 0.24 +1.1 55,737 - Ac3H11_168 0.24 -1.0 55,737 - Ac3H11_168 0.24 +0.2 55,799 + Ac3H11_168 0.39 -2.4 55,799 + Ac3H11_168 0.39 +1.0 55,799 + Ac3H11_168 0.39 +1.1 55,799 + Ac3H11_168 0.39 -0.2 55,799 + Ac3H11_168 0.39 +0.4 55,799 + Ac3H11_168 0.39 +0.9 55,799 + Ac3H11_168 0.39 +0.6 55,799 + Ac3H11_168 0.39 -1.5 55,799 + Ac3H11_168 0.39 -0.8 55,800 - Ac3H11_168 0.40 -0.2 55,800 - Ac3H11_168 0.40 -0.4 55,842 - Ac3H11_168 0.50 -0.1 56,091 - -0.3 56,112 + +0.1 56,112 + +3.7 56,112 + -0.5 56,112 + +1.9 56,113 - +0.3 56,113 - -1.4 56,169 + Ac3H11_169 0.11 +0.2 56,169 + Ac3H11_169 0.11 -0.5 56,270 - Ac3H11_169 0.21 -1.0 56,270 - Ac3H11_169 0.21 +1.9 56,270 - Ac3H11_169 0.21 -0.5 56,270 - Ac3H11_169 0.21 -0.8 56,434 - Ac3H11_169 0.37 -0.3 56,434 - Ac3H11_169 0.37 +1.1 56,443 - Ac3H11_169 0.37 -0.5 56,583 + Ac3H11_169 0.51 +0.5 56,716 - Ac3H11_169 0.63 -0.6 57,022 - -0.4
Or see this region's nucleotide sequence