Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2358 and Shewana3_2359 are separated by 78 nucleotides Shewana3_2359 and Shewana3_2360 are separated by 77 nucleotides Shewana3_2360 and Shewana3_2361 overlap by 13 nucleotides Shewana3_2361 and Shewana3_2362 are separated by 223 nucleotides
Shewana3_2358: Shewana3_2358 - hypothetical protein (RefSeq), at 2,837,441 to 2,837,677
_2358
Shewana3_2359: Shewana3_2359 - transposase IS3/IS911 family protein (RefSeq), at 2,837,756 to 2,838,001
_2359
Shewana3_2360: Shewana3_2360 - IS3 family transposase (RefSeq), at 2,838,079 to 2,838,426
_2360
Shewana3_2361: Shewana3_2361 - integrase catalytic subunit (RefSeq), at 2,838,414 to 2,838,917
_2361
Shewana3_2362: Shewana3_2362 - NnrS family protein (RefSeq), at 2,839,141 to 2,840,352
_2362
Position (kb)
2838
2839 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2837.754 kb on - strand at 2837.874 kb on + strand, within Shewana3_2359 at 2837.936 kb on - strand, within Shewana3_2359 at 2838.104 kb on + strand at 2838.429 kb on + strand at 2838.588 kb on + strand, within Shewana3_2361 at 2838.664 kb on + strand, within Shewana3_2361 at 2838.902 kb on - strand at 2838.932 kb on + strand at 2839.044 kb on + strand at 2839.344 kb on - strand, within Shewana3_2362 at 2839.564 kb on - strand, within Shewana3_2362 at 2839.854 kb on - strand, within Shewana3_2362 at 2839.856 kb on + strand, within Shewana3_2362 at 2839.909 kb on - strand, within Shewana3_2362
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,837,754 - -0.5 2,837,874 + Shewana3_2359 0.48 +1.8 2,837,936 - Shewana3_2359 0.73 +0.4 2,838,104 + +0.1 2,838,429 + -1.4 2,838,588 + Shewana3_2361 0.35 -0.2 2,838,664 + Shewana3_2361 0.50 -1.6 2,838,902 - +1.4 2,838,932 + -0.2 2,839,044 + -4.8 2,839,344 - Shewana3_2362 0.17 -0.4 2,839,564 - Shewana3_2362 0.35 -0.2 2,839,854 - Shewana3_2362 0.59 -1.4 2,839,856 + Shewana3_2362 0.59 +0.4 2,839,909 - Shewana3_2362 0.63 +0.4
Or see this region's nucleotide sequence