Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2268 and Shewana3_2269 are separated by 468 nucleotides Shewana3_2269 and ilvH overlap by 1 nucleotides ilvH and Shewana3_2271 are separated by 134 nucleotides
Shewana3_2268: Shewana3_2268 - Bcr/CflA subfamily drug resistance transporter (RefSeq), at 2,731,359 to 2,732,582
_2268
Shewana3_2269: Shewana3_2269 - acetolactate synthase 3 catalytic subunit (RefSeq), at 2,733,051 to 2,734,769
_2269
Shewana3_2270: ilvH - acetolactate synthase 3 regulatory subunit (RefSeq), at 2,734,769 to 2,735,263
ilvH
Shewana3_2271: Shewana3_2271 - heat shock protein Hsp20 (RefSeq), at 2,735,398 to 2,735,841
_2271
Position (kb)
2733
2734
2735 Strain fitness (log2 ratio)
-1
0
1 at 2732.404 kb on + strand, within Shewana3_2268 at 2733.138 kb on + strand at 2733.138 kb on + strand at 2733.146 kb on - strand at 2734.029 kb on + strand, within Shewana3_2269 at 2734.421 kb on + strand, within Shewana3_2269 at 2734.693 kb on - strand at 2734.695 kb on + strand at 2734.700 kb on + strand at 2735.123 kb on + strand, within ilvH at 2735.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,732,404 + Shewana3_2268 0.85 -0.1 2,733,138 + +0.9 2,733,138 + +0.6 2,733,146 - +0.7 2,734,029 + Shewana3_2269 0.57 +0.7 2,734,421 + Shewana3_2269 0.80 +0.3 2,734,693 - -1.6 2,734,695 + +0.6 2,734,700 + +0.2 2,735,123 + ilvH Shewana3_2270 0.72 +0.5 2,735,222 + +0.5
Or see this region's nucleotide sequence