Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2147 and Shewana3_2148 are separated by 70 nucleotides Shewana3_2148 and Shewana3_2149 are separated by 20 nucleotides Shewana3_2149 and Shewana3_2150 are separated by 12 nucleotides Shewana3_2150 and Shewana3_2151 are separated by 9 nucleotides
Shewana3_2147: Shewana3_2147 - deoxyguanosinetriphosphate triphosphohydrolase-like protein (RefSeq), at 2,571,878 to 2,573,218
_2147
Shewana3_2148: Shewana3_2148 - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (RefSeq), at 2,573,289 to 2,573,930
_2148
Shewana3_2149: Shewana3_2149 - phosphogluconate dehydratase (RefSeq), at 2,573,951 to 2,575,777
_2149
Shewana3_2150: Shewana3_2150 - 6-phosphogluconolactonase (RefSeq), at 2,575,790 to 2,576,488
_2150
Shewana3_2151: Shewana3_2151 - glucose-6-phosphate 1-dehydrogenase (RefSeq), at 2,576,498 to 2,577,970
_2151
Position (kb)
2573
2574
2575
2576 Strain fitness (log2 ratio)
-1
0
1 at 2572.996 kb on - strand, within Shewana3_2147 at 2573.153 kb on + strand at 2574.181 kb on - strand, within Shewana3_2149 at 2574.332 kb on - strand, within Shewana3_2149 at 2574.602 kb on - strand, within Shewana3_2149 at 2575.181 kb on - strand, within Shewana3_2149 at 2575.445 kb on - strand, within Shewana3_2149 at 2575.805 kb on - strand at 2576.006 kb on - strand, within Shewana3_2150 at 2576.242 kb on - strand, within Shewana3_2150 at 2576.373 kb on - strand, within Shewana3_2150
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,572,996 - Shewana3_2147 0.83 +1.5 2,573,153 + -0.1 2,574,181 - Shewana3_2149 0.13 -0.8 2,574,332 - Shewana3_2149 0.21 +0.5 2,574,602 - Shewana3_2149 0.36 +0.5 2,575,181 - Shewana3_2149 0.67 -0.1 2,575,445 - Shewana3_2149 0.82 -0.3 2,575,805 - +0.5 2,576,006 - Shewana3_2150 0.31 -1.0 2,576,242 - Shewana3_2150 0.65 -1.5 2,576,373 - Shewana3_2150 0.83 -0.5
Or see this region's nucleotide sequence