Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2134 and Shewana3_2135 are separated by 71 nucleotides Shewana3_2135 and Shewana3_2136 are separated by 271 nucleotides Shewana3_2136 and Shewana3_2137 are separated by 101 nucleotides Shewana3_2137 and Shewana3_2138 are separated by 11 nucleotides Shewana3_2138 and Shewana3_2139 are separated by 55 nucleotides
Shewana3_2134: Shewana3_2134 - phosphoglucomutase (RefSeq), at 2,559,733 to 2,561,385
_2134
Shewana3_2135: Shewana3_2135 - replication initiation regulator SeqA (RefSeq), at 2,561,457 to 2,562,008
_2135
Shewana3_2136: Shewana3_2136 - alpha/beta hydrolase fold (RefSeq), at 2,562,280 to 2,563,080
_2136
Shewana3_2137: Shewana3_2137 - hypothetical protein (RefSeq), at 2,563,182 to 2,563,403
_2137
Shewana3_2138: Shewana3_2138 - LexA regulated protein (RefSeq), at 2,563,415 to 2,563,687
_2138
Shewana3_2139: Shewana3_2139 - flavodoxin FldA (RefSeq), at 2,563,743 to 2,564,270
_2139
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-1
0
1 at 2561.465 kb on - strand at 2562.111 kb on - strand at 2562.211 kb on - strand at 2562.812 kb on + strand, within Shewana3_2136 at 2562.943 kb on - strand, within Shewana3_2136 at 2563.153 kb on - strand at 2563.371 kb on - strand, within Shewana3_2137 at 2563.537 kb on + strand, within Shewana3_2138 at 2563.638 kb on + strand, within Shewana3_2138 at 2563.710 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,561,465 - +0.4 2,562,111 - +0.7 2,562,211 - +0.9 2,562,812 + Shewana3_2136 0.66 -0.8 2,562,943 - Shewana3_2136 0.83 -0.7 2,563,153 - -0.7 2,563,371 - Shewana3_2137 0.85 +0.5 2,563,537 + Shewana3_2138 0.45 -0.3 2,563,638 + Shewana3_2138 0.82 -0.1 2,563,710 + -0.6
Or see this region's nucleotide sequence