Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2121 and Shewana3_R0089 are separated by 293 nucleotides Shewana3_R0089 and Shewana3_R0090 are separated by 49 nucleotides Shewana3_R0090 and Shewana3_R0091 are separated by 23 nucleotides Shewana3_R0091 and Shewana3_2122 are separated by 325 nucleotides Shewana3_2122 and Shewana3_2123 are separated by 206 nucleotides
Shewana3_2121: Shewana3_2121 - aromatic amino acid aminotransferase (RefSeq), at 2,544,569 to 2,545,762
_2121
Shewana3_R0089: Shewana3_R0089 - tRNA-Leu (RefSeq), at 2,546,056 to 2,546,142
_R0089
Shewana3_R0090: Shewana3_R0090 - tRNA-Cys (RefSeq), at 2,546,192 to 2,546,265
_R0090
Shewana3_R0091: Shewana3_R0091 - tRNA-Gly (RefSeq), at 2,546,289 to 2,546,364
_R0091
Shewana3_2122: Shewana3_2122 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,546,690 to 2,548,123
_2122
Shewana3_2123: Shewana3_2123 - hypothetical protein (RefSeq), at 2,548,330 to 2,549,217
_2123
Position (kb)
2546
2547
2548
2549 Strain fitness (log2 ratio)
-2
-1
0
1 at 2545.695 kb on + strand at 2545.814 kb on - strand at 2545.826 kb on + strand at 2546.002 kb on - strand at 2546.042 kb on - strand at 2546.614 kb on + strand at 2546.624 kb on + strand at 2546.918 kb on - strand, within Shewana3_2122 at 2547.243 kb on - strand, within Shewana3_2122 at 2547.684 kb on - strand, within Shewana3_2122 at 2547.801 kb on - strand, within Shewana3_2122 at 2548.370 kb on + strand at 2548.790 kb on + strand, within Shewana3_2123 at 2548.939 kb on - strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,545,695 + +1.5 2,545,814 - -2.0 2,545,826 + +0.5 2,546,002 - +1.5 2,546,042 - +1.0 2,546,614 + +0.6 2,546,624 + +0.8 2,546,918 - Shewana3_2122 0.16 -1.3 2,547,243 - Shewana3_2122 0.39 +0.5 2,547,684 - Shewana3_2122 0.69 -1.1 2,547,801 - Shewana3_2122 0.77 -0.8 2,548,370 + +1.5 2,548,790 + Shewana3_2123 0.52 +0.5 2,548,939 - Shewana3_2123 0.69 -0.5 2,549,080 + Shewana3_2123 0.84 +0.2 2,549,080 + Shewana3_2123 0.84 -0.1
Or see this region's nucleotide sequence