Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0072 and Shewana3_R0073 are separated by 175 nucleotides Shewana3_R0073 and Shewana3_1584 are separated by 259 nucleotides Shewana3_1584 and cpdB are separated by 226 nucleotides
Shewana3_R0072: Shewana3_R0072 - 23S ribosomal RNA (RefSeq), at 1,855,447 to 1,858,341
_R0072
Shewana3_R0073: Shewana3_R0073 - 5S ribosomal RNA (RefSeq), at 1,858,517 to 1,858,636
_R0073
Shewana3_1584: Shewana3_1584 - hypothetical protein (RefSeq), at 1,858,896 to 1,859,612
_1584
Shewana3_1585: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 1,859,839 to 1,861,896
cpdB
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-1
0
1 at 1858.848 kb on + strand at 1859.008 kb on + strand, within Shewana3_1584 at 1859.037 kb on - strand, within Shewana3_1584 at 1859.054 kb on - strand, within Shewana3_1584 at 1859.087 kb on + strand, within Shewana3_1584 at 1859.281 kb on + strand, within Shewana3_1584 at 1859.672 kb on + strand at 1859.711 kb on - strand at 1860.223 kb on + strand, within cpdB at 1860.233 kb on + strand at 1860.240 kb on - strand, within cpdB at 1860.265 kb on - strand, within cpdB at 1860.419 kb on + strand, within cpdB at 1860.557 kb on + strand, within cpdB
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 1,858,848 + +0.1 1,859,008 + Shewana3_1584 0.16 -0.8 1,859,037 - Shewana3_1584 0.20 -0.6 1,859,054 - Shewana3_1584 0.22 -0.1 1,859,087 + Shewana3_1584 0.27 -0.6 1,859,281 + Shewana3_1584 0.54 -1.4 1,859,672 + -0.1 1,859,711 - +1.4 1,860,223 + cpdB Shewana3_1585 0.19 +1.4 1,860,233 + +1.4 1,860,240 - cpdB Shewana3_1585 0.19 +0.3 1,860,265 - cpdB Shewana3_1585 0.21 -0.1 1,860,419 + cpdB Shewana3_1585 0.28 +1.4 1,860,557 + cpdB Shewana3_1585 0.35 +1.4
Or see this region's nucleotide sequence