Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliN are separated by 12 nucleotides fliN and Shewana3_1351 are separated by 33 nucleotides Shewana3_1351 and fliP overlap by 4 nucleotides fliP and Shewana3_1353 are separated by 17 nucleotides
Shewana3_1349: fliM - flagellar motor switch protein FliM (RefSeq), at 1,562,900 to 1,563,931
fliM
Shewana3_1350: fliN - flagellar motor switch protein (RefSeq), at 1,563,944 to 1,564,318
fliN
Shewana3_1351: Shewana3_1351 - flagellar biosynthesis protein, FliO (RefSeq), at 1,564,352 to 1,564,759
_1351
Shewana3_1352: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,564,756 to 1,565,502
fliP
Shewana3_1353: Shewana3_1353 - flagellar biosynthetic protein FliQ (RefSeq), at 1,565,520 to 1,565,789
_1353
Position (kb)
1564
1565 Strain fitness (log2 ratio)
-1
0
1 at 1563.608 kb on - strand, within fliM at 1563.635 kb on + strand, within fliM at 1563.692 kb on + strand, within fliM at 1563.961 kb on - strand at 1563.961 kb on - strand at 1563.961 kb on - strand at 1564.005 kb on - strand, within fliN at 1564.005 kb on - strand, within fliN at 1564.013 kb on - strand, within fliN at 1564.076 kb on + strand, within fliN at 1564.171 kb on - strand, within fliN at 1564.241 kb on + strand, within fliN at 1564.337 kb on + strand at 1564.337 kb on + strand at 1564.349 kb on + strand at 1564.415 kb on + strand, within Shewana3_1351 at 1564.558 kb on + strand, within Shewana3_1351 at 1564.731 kb on + strand at 1564.783 kb on - strand at 1565.442 kb on + strand at 1565.520 kb on - strand at 1565.564 kb on - strand, within Shewana3_1353
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 1,563,608 - fliM Shewana3_1349 0.69 -0.3 1,563,635 + fliM Shewana3_1349 0.71 +0.8 1,563,692 + fliM Shewana3_1349 0.77 +1.4 1,563,961 - +0.3 1,563,961 - +1.3 1,563,961 - -0.3 1,564,005 - fliN Shewana3_1350 0.16 +1.6 1,564,005 - fliN Shewana3_1350 0.16 +1.3 1,564,013 - fliN Shewana3_1350 0.18 -0.3 1,564,076 + fliN Shewana3_1350 0.35 +1.3 1,564,171 - fliN Shewana3_1350 0.61 -1.0 1,564,241 + fliN Shewana3_1350 0.79 +1.2 1,564,337 + -0.1 1,564,337 + -0.4 1,564,349 + +0.2 1,564,415 + Shewana3_1351 0.15 +0.7 1,564,558 + Shewana3_1351 0.50 +0.2 1,564,731 + -0.3 1,564,783 - +1.3 1,565,442 + +0.4 1,565,520 - +0.3 1,565,564 - Shewana3_1353 0.16 -0.7
Or see this region's nucleotide sequence