Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1335 and Shewana3_1336 are separated by 18 nucleotides Shewana3_1336 and fliS are separated by 6 nucleotides fliS and Shewana3_1338 are separated by 286 nucleotides
Shewana3_1335: Shewana3_1335 - flagellar hook-associated 2 domain-containing protein (RefSeq), at 1,547,975 to 1,549,345
_1335
Shewana3_1336: Shewana3_1336 - hypothetical protein (RefSeq), at 1,549,364 to 1,549,693
_1336
Shewana3_1337: fliS - flagellar protein FliS (RefSeq), at 1,549,700 to 1,550,113
fliS
Shewana3_1338: Shewana3_1338 - sigma-54 dependent trancsriptional regulator (RefSeq), at 1,550,400 to 1,551,833
_1338
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1548.794 kb on + strand, within Shewana3_1335 at 1548.794 kb on + strand, within Shewana3_1335 at 1549.254 kb on + strand at 1549.298 kb on - strand at 1549.321 kb on + strand at 1549.512 kb on + strand, within Shewana3_1336 at 1549.562 kb on + strand, within Shewana3_1336 at 1549.571 kb on - strand, within Shewana3_1336 at 1549.694 kb on + strand at 1549.742 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.819 kb on - strand, within fliS at 1550.052 kb on + strand, within fliS at 1550.143 kb on + strand at 1550.204 kb on - strand at 1550.280 kb on + strand at 1550.298 kb on - strand at 1550.305 kb on - strand at 1550.698 kb on + strand, within Shewana3_1338 at 1550.706 kb on - strand, within Shewana3_1338 at 1550.879 kb on - strand, within Shewana3_1338
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 1,548,794 + Shewana3_1335 0.60 +1.3 1,548,794 + Shewana3_1335 0.60 +1.9 1,549,254 + +1.9 1,549,298 - +1.3 1,549,321 + +0.5 1,549,512 + Shewana3_1336 0.45 +1.7 1,549,562 + Shewana3_1336 0.60 +1.1 1,549,571 - Shewana3_1336 0.63 +0.3 1,549,694 + +0.5 1,549,742 + fliS Shewana3_1337 0.10 +1.1 1,549,811 + fliS Shewana3_1337 0.27 +1.2 1,549,811 + fliS Shewana3_1337 0.27 +0.9 1,549,811 + fliS Shewana3_1337 0.27 +2.1 1,549,819 - fliS Shewana3_1337 0.29 -0.3 1,550,052 + fliS Shewana3_1337 0.85 +0.4 1,550,143 + +0.2 1,550,204 - +0.5 1,550,280 + -1.3 1,550,298 - +3.3 1,550,305 - +2.4 1,550,698 + Shewana3_1338 0.21 +1.9 1,550,706 - Shewana3_1338 0.21 +0.3 1,550,879 - Shewana3_1338 0.33 -0.7
Or see this region's nucleotide sequence