Strain Fitness in Shewanella sp. ANA-3 around Shewana3_0011

Experiment: syringaldehyde 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_0010 and recF are separated by 162 nucleotidesrecF and Shewana3_0012 are separated by 16 nucleotides Shewana3_0010: Shewana3_0010 - DNA polymerase III, beta subunit (RefSeq), at 11,054 to 12,154 _0010 Shewana3_0011: recF - recombination protein F (RefSeq), at 12,317 to 13,399 recF Shewana3_0012: Shewana3_0012 - DNA gyrase subunit B (RefSeq), at 13,416 to 15,833 _0012 Position (kb) 12 13 14Strain fitness (log2 ratio) -3 -2 -1 0 1at 12.213 kb on + strandat 12.213 kb on + strandat 12.221 kb on - strandat 12.285 kb on - strandat 12.285 kb on - strandat 12.329 kb on - strandat 12.365 kb on + strandat 12.373 kb on - strandat 12.433 kb on + strand, within recFat 12.511 kb on + strand, within recFat 12.519 kb on - strand, within recFat 12.524 kb on - strand, within recFat 12.541 kb on - strand, within recFat 12.542 kb on - strand, within recFat 12.877 kb on + strand, within recFat 12.885 kb on - strand, within recFat 12.895 kb on + strand, within recFat 12.922 kb on + strand, within recFat 12.930 kb on - strand, within recFat 12.967 kb on + strand, within recFat 13.005 kb on + strand, within recFat 13.042 kb on + strand, within recF

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Per-strain Table

Position Strand Gene LocusTag Fraction syringaldehyde 1 mM
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12,213 + +0.0
12,213 + +0.1
12,221 - -0.2
12,285 - -1.3
12,285 - -3.1
12,329 - -1.5
12,365 + -1.4
12,373 - -2.4
12,433 + recF Shewana3_0011 0.11 -2.0
12,511 + recF Shewana3_0011 0.18 -1.8
12,519 - recF Shewana3_0011 0.19 -2.6
12,524 - recF Shewana3_0011 0.19 -3.2
12,541 - recF Shewana3_0011 0.21 -1.3
12,542 - recF Shewana3_0011 0.21 -1.8
12,877 + recF Shewana3_0011 0.52 -0.9
12,885 - recF Shewana3_0011 0.52 -1.9
12,895 + recF Shewana3_0011 0.53 -1.9
12,922 + recF Shewana3_0011 0.56 -2.9
12,930 - recF Shewana3_0011 0.57 -0.8
12,967 + recF Shewana3_0011 0.60 -2.8
13,005 + recF Shewana3_0011 0.64 -1.1
13,042 + recF Shewana3_0011 0.67 -2.0

Or see this region's nucleotide sequence