Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4535 and BT4536 are separated by 2 nucleotides BT4536 and BT4537 are separated by 4 nucleotides BT4537 and BT4538 are separated by 16 nucleotides
BT4535: BT4535 - Type I restriction enzyme EcoR124II R protein (NCBI ptt file), at 5,952,578 to 5,955,406
BT4535
BT4536: BT4536 - conserved hypothetical protein (NCBI ptt file), at 5,955,409 to 5,956,332
BT4536
BT4537: BT4537 - conserved hypothetical protein (NCBI ptt file), at 5,956,337 to 5,958,040
BT4537
BT4538: BT4538 - Type I restriction enzyme EcoR124II M protein (NCBI ptt file), at 5,958,057 to 5,959,610
BT4538
Position (kb)
5956
5957
5958
5959 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5955.405 kb on - strand at 5955.418 kb on + strand at 5955.441 kb on - strand at 5955.450 kb on - strand at 5955.641 kb on + strand, within BT4536 at 5955.642 kb on - strand, within BT4536 at 5955.661 kb on + strand, within BT4536 at 5955.661 kb on + strand, within BT4536 at 5955.661 kb on + strand, within BT4536 at 5955.702 kb on - strand, within BT4536 at 5955.833 kb on + strand, within BT4536 at 5955.840 kb on - strand, within BT4536 at 5955.840 kb on - strand, within BT4536 at 5955.840 kb on - strand, within BT4536 at 5955.888 kb on - strand, within BT4536 at 5955.888 kb on - strand, within BT4536 at 5955.928 kb on - strand, within BT4536 at 5955.973 kb on - strand, within BT4536 at 5955.989 kb on + strand, within BT4536 at 5955.990 kb on - strand, within BT4536 at 5956.022 kb on - strand, within BT4536 at 5956.063 kb on - strand, within BT4536 at 5956.066 kb on - strand, within BT4536 at 5956.070 kb on + strand, within BT4536 at 5956.073 kb on - strand, within BT4536 at 5956.168 kb on - strand, within BT4536 at 5956.197 kb on + strand, within BT4536 at 5956.198 kb on - strand, within BT4536 at 5956.281 kb on - strand at 5956.324 kb on - strand at 5956.337 kb on - strand at 5956.343 kb on + strand at 5956.354 kb on + strand at 5956.355 kb on - strand at 5956.462 kb on + strand at 5956.465 kb on + strand at 5956.465 kb on + strand at 5956.466 kb on - strand at 5956.466 kb on - strand at 5956.484 kb on - strand at 5956.539 kb on - strand, within BT4537 at 5956.547 kb on - strand, within BT4537 at 5956.580 kb on - strand, within BT4537 at 5956.589 kb on - strand, within BT4537 at 5956.615 kb on - strand, within BT4537 at 5956.615 kb on - strand, within BT4537 at 5956.652 kb on - strand, within BT4537 at 5956.657 kb on + strand, within BT4537 at 5956.658 kb on - strand, within BT4537 at 5956.666 kb on - strand, within BT4537 at 5956.719 kb on + strand, within BT4537 at 5956.748 kb on - strand, within BT4537 at 5956.825 kb on - strand, within BT4537 at 5956.853 kb on + strand, within BT4537 at 5956.968 kb on + strand, within BT4537 at 5956.968 kb on + strand, within BT4537 at 5956.969 kb on - strand, within BT4537 at 5956.981 kb on - strand, within BT4537 at 5957.009 kb on + strand, within BT4537 at 5957.010 kb on - strand, within BT4537 at 5957.029 kb on - strand, within BT4537 at 5957.067 kb on - strand, within BT4537 at 5957.067 kb on - strand, within BT4537 at 5957.099 kb on - strand, within BT4537 at 5957.099 kb on - strand, within BT4537 at 5957.141 kb on - strand, within BT4537 at 5957.210 kb on + strand, within BT4537 at 5957.210 kb on + strand, within BT4537 at 5957.210 kb on + strand, within BT4537 at 5957.230 kb on + strand, within BT4537 at 5957.233 kb on - strand, within BT4537 at 5957.233 kb on - strand, within BT4537 at 5957.238 kb on - strand, within BT4537 at 5957.269 kb on - strand, within BT4537 at 5957.276 kb on - strand, within BT4537 at 5957.323 kb on - strand, within BT4537 at 5957.333 kb on - strand, within BT4537 at 5957.347 kb on + strand, within BT4537 at 5957.380 kb on - strand, within BT4537 at 5957.421 kb on - strand, within BT4537 at 5957.435 kb on - strand, within BT4537 at 5957.435 kb on - strand, within BT4537 at 5957.505 kb on + strand, within BT4537 at 5957.506 kb on - strand, within BT4537 at 5957.506 kb on - strand, within BT4537 at 5957.510 kb on - strand, within BT4537 at 5957.536 kb on + strand, within BT4537 at 5957.567 kb on + strand, within BT4537 at 5957.568 kb on - strand, within BT4537 at 5957.655 kb on - strand, within BT4537 at 5957.655 kb on - strand, within BT4537 at 5957.655 kb on - strand, within BT4537 at 5957.682 kb on + strand, within BT4537 at 5957.723 kb on + strand, within BT4537 at 5957.737 kb on + strand, within BT4537 at 5957.738 kb on - strand, within BT4537 at 5957.738 kb on - strand, within BT4537 at 5957.742 kb on - strand, within BT4537 at 5957.796 kb on + strand, within BT4537 at 5957.796 kb on + strand, within BT4537 at 5957.800 kb on + strand, within BT4537 at 5957.824 kb on + strand, within BT4537 at 5957.882 kb on - strand at 5957.960 kb on - strand at 5957.960 kb on - strand at 5957.970 kb on - strand at 5958.125 kb on + strand at 5958.152 kb on + strand at 5958.152 kb on + strand at 5958.230 kb on + strand, within BT4538 at 5958.230 kb on + strand, within BT4538 at 5958.231 kb on - strand, within BT4538 at 5958.273 kb on - strand, within BT4538 at 5958.322 kb on - strand, within BT4538 at 5958.365 kb on + strand at 5958.389 kb on - strand, within BT4538 at 5958.429 kb on - strand, within BT4538 at 5958.519 kb on - strand, within BT4538 at 5958.575 kb on - strand, within BT4538 at 5958.596 kb on + strand, within BT4538 at 5958.597 kb on - strand, within BT4538 at 5958.620 kb on + strand, within BT4538 at 5958.670 kb on - strand, within BT4538 at 5958.672 kb on - strand, within BT4538 at 5958.697 kb on + strand, within BT4538 at 5958.697 kb on + strand, within BT4538 at 5958.742 kb on - strand, within BT4538 at 5958.742 kb on - strand, within BT4538 at 5958.772 kb on + strand, within BT4538 at 5958.788 kb on + strand, within BT4538 at 5958.789 kb on - strand, within BT4538 at 5958.837 kb on + strand, within BT4538 at 5958.840 kb on - strand, within BT4538 at 5958.857 kb on + strand, within BT4538 at 5958.909 kb on - strand, within BT4538
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,955,405 - -1.6 5,955,418 + -2.0 5,955,441 - -1.2 5,955,450 - +1.4 5,955,641 + BT4536 0.25 +0.3 5,955,642 - BT4536 0.25 -0.5 5,955,661 + BT4536 0.27 -2.3 5,955,661 + BT4536 0.27 +1.4 5,955,661 + BT4536 0.27 +0.8 5,955,702 - BT4536 0.32 -0.2 5,955,833 + BT4536 0.46 +1.7 5,955,840 - BT4536 0.47 -2.5 5,955,840 - BT4536 0.47 -1.9 5,955,840 - BT4536 0.47 -2.2 5,955,888 - BT4536 0.52 -0.7 5,955,888 - BT4536 0.52 -2.3 5,955,928 - BT4536 0.56 -2.2 5,955,973 - BT4536 0.61 -1.5 5,955,989 + BT4536 0.63 -1.2 5,955,990 - BT4536 0.63 -2.1 5,956,022 - BT4536 0.66 -1.1 5,956,063 - BT4536 0.71 -2.5 5,956,066 - BT4536 0.71 -2.5 5,956,070 + BT4536 0.72 +0.7 5,956,073 - BT4536 0.72 -2.3 5,956,168 - BT4536 0.82 -1.3 5,956,197 + BT4536 0.85 -0.2 5,956,198 - BT4536 0.85 -0.9 5,956,281 - -0.2 5,956,324 - -1.8 5,956,337 - -3.2 5,956,343 + -0.2 5,956,354 + -1.0 5,956,355 - -1.4 5,956,462 + -0.6 5,956,465 + +0.7 5,956,465 + -0.9 5,956,466 - +0.8 5,956,466 - -2.8 5,956,484 - +1.4 5,956,539 - BT4537 0.12 -1.6 5,956,547 - BT4537 0.12 +0.3 5,956,580 - BT4537 0.14 -3.1 5,956,589 - BT4537 0.15 -2.0 5,956,615 - BT4537 0.16 -1.9 5,956,615 - BT4537 0.16 -1.2 5,956,652 - BT4537 0.18 -1.6 5,956,657 + BT4537 0.19 -1.3 5,956,658 - BT4537 0.19 +1.2 5,956,666 - BT4537 0.19 -1.6 5,956,719 + BT4537 0.22 +2.4 5,956,748 - BT4537 0.24 -1.1 5,956,825 - BT4537 0.29 -2.1 5,956,853 + BT4537 0.30 -2.9 5,956,968 + BT4537 0.37 +0.5 5,956,968 + BT4537 0.37 -2.8 5,956,969 - BT4537 0.37 -1.1 5,956,981 - BT4537 0.38 -2.7 5,957,009 + BT4537 0.39 -2.5 5,957,010 - BT4537 0.39 -1.8 5,957,029 - BT4537 0.41 -0.9 5,957,067 - BT4537 0.43 -0.2 5,957,067 - BT4537 0.43 -1.3 5,957,099 - BT4537 0.45 -0.5 5,957,099 - BT4537 0.45 +1.4 5,957,141 - BT4537 0.47 -1.4 5,957,210 + BT4537 0.51 +0.1 5,957,210 + BT4537 0.51 -1.5 5,957,210 + BT4537 0.51 -1.0 5,957,230 + BT4537 0.52 -0.5 5,957,233 - BT4537 0.53 -0.4 5,957,233 - BT4537 0.53 -2.4 5,957,238 - BT4537 0.53 -1.9 5,957,269 - BT4537 0.55 -1.6 5,957,276 - BT4537 0.55 -1.6 5,957,323 - BT4537 0.58 -3.5 5,957,333 - BT4537 0.58 -1.2 5,957,347 + BT4537 0.59 +0.4 5,957,380 - BT4537 0.61 -1.2 5,957,421 - BT4537 0.64 -1.2 5,957,435 - BT4537 0.64 -2.0 5,957,435 - BT4537 0.64 -1.9 5,957,505 + BT4537 0.69 -1.6 5,957,506 - BT4537 0.69 +1.4 5,957,506 - BT4537 0.69 -1.1 5,957,510 - BT4537 0.69 -2.8 5,957,536 + BT4537 0.70 -1.4 5,957,567 + BT4537 0.72 -1.2 5,957,568 - BT4537 0.72 -2.2 5,957,655 - BT4537 0.77 -3.0 5,957,655 - BT4537 0.77 +0.1 5,957,655 - BT4537 0.77 -1.3 5,957,682 + BT4537 0.79 -0.6 5,957,723 + BT4537 0.81 -0.2 5,957,737 + BT4537 0.82 -0.3 5,957,738 - BT4537 0.82 -0.6 5,957,738 - BT4537 0.82 -0.5 5,957,742 - BT4537 0.82 -1.5 5,957,796 + BT4537 0.86 -0.2 5,957,796 + BT4537 0.86 -2.5 5,957,800 + BT4537 0.86 +0.7 5,957,824 + BT4537 0.87 -1.7 5,957,882 - +0.3 5,957,960 - -1.8 5,957,960 - +1.4 5,957,970 - -3.3 5,958,125 + +1.4 5,958,152 + -2.1 5,958,152 + -1.2 5,958,230 + BT4538 0.11 -1.9 5,958,230 + BT4538 0.11 +0.8 5,958,231 - BT4538 0.11 +0.4 5,958,273 - BT4538 0.14 -1.3 5,958,322 - BT4538 0.17 -0.5 5,958,365 + +1.4 5,958,389 - BT4538 0.21 +1.4 5,958,429 - BT4538 0.24 -0.3 5,958,519 - BT4538 0.30 +0.2 5,958,575 - BT4538 0.33 -1.2 5,958,596 + BT4538 0.35 -0.3 5,958,597 - BT4538 0.35 -1.4 5,958,620 + BT4538 0.36 -3.7 5,958,670 - BT4538 0.39 +0.2 5,958,672 - BT4538 0.40 -2.3 5,958,697 + BT4538 0.41 -0.9 5,958,697 + BT4538 0.41 -3.0 5,958,742 - BT4538 0.44 +1.0 5,958,742 - BT4538 0.44 -0.9 5,958,772 + BT4538 0.46 +1.4 5,958,788 + BT4538 0.47 -0.9 5,958,789 - BT4538 0.47 -0.6 5,958,837 + BT4538 0.50 +0.4 5,958,840 - BT4538 0.50 -0.4 5,958,857 + BT4538 0.51 +0.8 5,958,909 - BT4538 0.55 -1.6
Or see this region's nucleotide sequence