Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4521 and BT4522 are separated by 6 nucleotides BT4522 and BT4523 overlap by 6 nucleotides BT4523 and BT4524 overlap by 14 nucleotides BT4524 and BT4525 are separated by 208 nucleotides
BT4521: BT4521 - integrase (NCBI ptt file), at 5,941,017 to 5,941,820
BT4521
BT4522: BT4522 - Type I restriction-modification enzyme (NCBI ptt file), at 5,941,827 to 5,942,354
BT4522
BT4523: BT4523 - Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file), at 5,942,349 to 5,943,533
BT4523
BT4524: BT4524 - conserved hypothetical protein (NCBI ptt file), at 5,943,520 to 5,943,810
BT4524
BT4525: BT4525 - hypothetical protein (NCBI ptt file), at 5,944,019 to 5,944,303
BT4525
Position (kb)
5942
5943
5944 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5941.357 kb on - strand, within BT4521 at 5941.365 kb on + strand, within BT4521 at 5941.471 kb on - strand, within BT4521 at 5941.481 kb on - strand, within BT4521 at 5941.491 kb on - strand, within BT4521 at 5941.507 kb on - strand, within BT4521 at 5941.573 kb on - strand, within BT4521 at 5941.632 kb on - strand, within BT4521 at 5941.688 kb on + strand, within BT4521 at 5941.689 kb on - strand, within BT4521 at 5941.691 kb on - strand, within BT4521 at 5941.764 kb on - strand at 5941.833 kb on + strand at 5941.858 kb on + strand at 5941.858 kb on + strand at 5941.858 kb on + strand at 5941.863 kb on + strand at 5941.866 kb on - strand at 5941.878 kb on - strand at 5941.878 kb on - strand at 5941.919 kb on - strand, within BT4522 at 5941.921 kb on + strand, within BT4522 at 5942.029 kb on - strand, within BT4522 at 5942.114 kb on + strand, within BT4522 at 5942.136 kb on - strand, within BT4522 at 5942.161 kb on - strand, within BT4522 at 5942.164 kb on + strand, within BT4522 at 5942.167 kb on + strand, within BT4522 at 5942.208 kb on - strand, within BT4522 at 5942.257 kb on + strand, within BT4522 at 5942.267 kb on + strand, within BT4522 at 5942.277 kb on + strand, within BT4522 at 5942.299 kb on + strand, within BT4522 at 5942.307 kb on - strand at 5942.308 kb on + strand at 5942.330 kb on - strand at 5942.330 kb on - strand at 5942.339 kb on - strand at 5942.352 kb on + strand at 5942.364 kb on + strand at 5942.406 kb on - strand at 5942.439 kb on + strand at 5942.439 kb on + strand at 5942.439 kb on + strand at 5942.440 kb on - strand at 5942.491 kb on - strand, within BT4523 at 5942.503 kb on - strand, within BT4523 at 5942.510 kb on + strand, within BT4523 at 5942.510 kb on + strand, within BT4523 at 5942.543 kb on + strand, within BT4523 at 5942.544 kb on - strand, within BT4523 at 5942.544 kb on - strand, within BT4523 at 5942.577 kb on + strand, within BT4523 at 5942.582 kb on + strand, within BT4523 at 5942.632 kb on - strand, within BT4523 at 5942.650 kb on + strand, within BT4523 at 5942.650 kb on + strand, within BT4523 at 5942.651 kb on - strand, within BT4523 at 5942.654 kb on + strand, within BT4523 at 5942.720 kb on - strand, within BT4523 at 5942.732 kb on - strand, within BT4523 at 5942.751 kb on - strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.976 kb on + strand, within BT4523 at 5942.984 kb on + strand, within BT4523 at 5942.991 kb on + strand, within BT4523 at 5943.021 kb on + strand, within BT4523 at 5943.030 kb on + strand, within BT4523 at 5943.050 kb on + strand, within BT4523 at 5943.054 kb on - strand, within BT4523 at 5943.081 kb on + strand, within BT4523 at 5943.082 kb on - strand, within BT4523 at 5943.082 kb on - strand, within BT4523 at 5943.143 kb on - strand, within BT4523 at 5943.143 kb on - strand, within BT4523 at 5943.200 kb on + strand, within BT4523 at 5943.201 kb on - strand, within BT4523 at 5943.202 kb on - strand, within BT4523 at 5943.246 kb on + strand, within BT4523 at 5943.279 kb on - strand, within BT4523 at 5943.311 kb on + strand, within BT4523 at 5943.312 kb on - strand, within BT4523 at 5943.355 kb on - strand, within BT4523 at 5943.377 kb on + strand, within BT4523 at 5943.377 kb on + strand, within BT4523 at 5943.378 kb on - strand, within BT4523 at 5943.378 kb on - strand, within BT4523 at 5943.380 kb on - strand, within BT4523 at 5943.425 kb on + strand at 5943.426 kb on - strand at 5943.454 kb on - strand at 5943.533 kb on + strand at 5943.533 kb on + strand at 5943.535 kb on + strand at 5943.536 kb on - strand at 5943.595 kb on + strand, within BT4524 at 5943.595 kb on + strand, within BT4524 at 5943.599 kb on + strand, within BT4524 at 5943.640 kb on + strand, within BT4524 at 5943.670 kb on + strand, within BT4524 at 5943.779 kb on - strand, within BT4524 at 5943.798 kb on + strand at 5943.889 kb on + strand at 5943.978 kb on + strand at 5944.034 kb on + strand at 5944.035 kb on - strand at 5944.037 kb on + strand at 5944.095 kb on + strand, within BT4525 at 5944.096 kb on - strand, within BT4525 at 5944.159 kb on + strand, within BT4525 at 5944.160 kb on - strand, within BT4525 at 5944.266 kb on - strand, within BT4525 at 5944.312 kb on - strand at 5944.318 kb on - strand at 5944.318 kb on - strand at 5944.323 kb on + strand at 5944.324 kb on - strand at 5944.368 kb on - strand at 5944.401 kb on - strand at 5944.450 kb on + strand at 5944.454 kb on + strand at 5944.454 kb on + strand at 5944.455 kb on - strand at 5944.455 kb on - strand at 5944.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,941,357 - BT4521 0.42 -0.8 5,941,365 + BT4521 0.43 +1.4 5,941,471 - BT4521 0.56 +0.4 5,941,481 - BT4521 0.58 -0.0 5,941,491 - BT4521 0.59 -1.9 5,941,507 - BT4521 0.61 +0.5 5,941,573 - BT4521 0.69 +0.4 5,941,632 - BT4521 0.76 -2.8 5,941,688 + BT4521 0.83 -0.2 5,941,689 - BT4521 0.84 -2.6 5,941,691 - BT4521 0.84 -0.6 5,941,764 - +0.1 5,941,833 + -2.4 5,941,858 + -0.5 5,941,858 + -0.3 5,941,858 + -0.0 5,941,863 + +2.2 5,941,866 - +0.7 5,941,878 - +1.4 5,941,878 - +0.7 5,941,919 - BT4522 0.17 -1.2 5,941,921 + BT4522 0.18 +0.4 5,942,029 - BT4522 0.38 -1.4 5,942,114 + BT4522 0.54 -2.3 5,942,136 - BT4522 0.59 -0.2 5,942,161 - BT4522 0.63 +1.4 5,942,164 + BT4522 0.64 +0.6 5,942,167 + BT4522 0.64 +0.2 5,942,208 - BT4522 0.72 -0.4 5,942,257 + BT4522 0.81 -0.7 5,942,267 + BT4522 0.83 -2.4 5,942,277 + BT4522 0.85 -1.2 5,942,299 + BT4522 0.89 +0.6 5,942,307 - -1.9 5,942,308 + -0.7 5,942,330 - +0.6 5,942,330 - +1.4 5,942,339 - -2.0 5,942,352 + -2.5 5,942,364 + -0.5 5,942,406 - -0.0 5,942,439 + -1.2 5,942,439 + -1.4 5,942,439 + -1.0 5,942,440 - -1.2 5,942,491 - BT4523 0.12 -0.9 5,942,503 - BT4523 0.13 +1.4 5,942,510 + BT4523 0.14 +1.4 5,942,510 + BT4523 0.14 +1.4 5,942,543 + BT4523 0.16 -0.9 5,942,544 - BT4523 0.16 -2.5 5,942,544 - BT4523 0.16 -3.2 5,942,577 + BT4523 0.19 +0.3 5,942,582 + BT4523 0.20 -0.3 5,942,632 - BT4523 0.24 -1.0 5,942,650 + BT4523 0.25 +0.8 5,942,650 + BT4523 0.25 +1.5 5,942,651 - BT4523 0.25 -0.6 5,942,654 + BT4523 0.26 -1.7 5,942,720 - BT4523 0.31 -0.4 5,942,732 - BT4523 0.32 +1.4 5,942,751 - BT4523 0.34 -0.4 5,942,752 + BT4523 0.34 -1.0 5,942,752 + BT4523 0.34 -0.5 5,942,752 + BT4523 0.34 -1.5 5,942,976 + BT4523 0.53 +2.4 5,942,984 + BT4523 0.54 -1.2 5,942,991 + BT4523 0.54 -1.2 5,943,021 + BT4523 0.57 -0.8 5,943,030 + BT4523 0.57 +3.0 5,943,050 + BT4523 0.59 -1.6 5,943,054 - BT4523 0.59 +0.4 5,943,081 + BT4523 0.62 -2.0 5,943,082 - BT4523 0.62 -0.3 5,943,082 - BT4523 0.62 -0.4 5,943,143 - BT4523 0.67 +0.1 5,943,143 - BT4523 0.67 -1.7 5,943,200 + BT4523 0.72 -0.4 5,943,201 - BT4523 0.72 -1.2 5,943,202 - BT4523 0.72 -1.0 5,943,246 + BT4523 0.76 -0.3 5,943,279 - BT4523 0.78 -0.6 5,943,311 + BT4523 0.81 +0.7 5,943,312 - BT4523 0.81 -0.1 5,943,355 - BT4523 0.85 -1.5 5,943,377 + BT4523 0.87 +0.5 5,943,377 + BT4523 0.87 -0.2 5,943,378 - BT4523 0.87 +0.7 5,943,378 - BT4523 0.87 -0.7 5,943,380 - BT4523 0.87 -2.3 5,943,425 + +1.0 5,943,426 - -0.9 5,943,454 - -0.2 5,943,533 + -0.9 5,943,533 + +0.4 5,943,535 + +0.2 5,943,536 - -1.1 5,943,595 + BT4524 0.26 +0.1 5,943,595 + BT4524 0.26 -1.7 5,943,599 + BT4524 0.27 -1.3 5,943,640 + BT4524 0.41 +0.6 5,943,670 + BT4524 0.52 -0.7 5,943,779 - BT4524 0.89 -1.1 5,943,798 + +2.0 5,943,889 + -1.9 5,943,978 + +2.0 5,944,034 + -1.4 5,944,035 - -0.3 5,944,037 + -0.5 5,944,095 + BT4525 0.27 -4.9 5,944,096 - BT4525 0.27 -1.8 5,944,159 + BT4525 0.49 +0.3 5,944,160 - BT4525 0.49 +0.4 5,944,266 - BT4525 0.87 +0.8 5,944,312 - +0.5 5,944,318 - +0.4 5,944,318 - +1.0 5,944,323 + -2.4 5,944,324 - +1.6 5,944,368 - -0.9 5,944,401 - -1.4 5,944,450 + -1.6 5,944,454 + +0.7 5,944,454 + +0.7 5,944,455 - -0.4 5,944,455 - -0.8 5,944,458 - -2.6
Or see this region's nucleotide sequence