Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4430 and BT4431 are separated by 195 nucleotides BT4431 and BT4432 are separated by 4 nucleotides
BT4430: BT4430 - cytochrome c biogenesis protein (ccsA) (NCBI ptt file), at 5,835,424 to 5,837,859
BT4430
BT4431: BT4431 - hypothetical protein (NCBI ptt file), at 5,838,055 to 5,839,854
BT4431
BT4432: BT4432 - putative non-specific DNA-binding protein HU-1 (NCBI ptt file), at 5,839,859 to 5,840,272
BT4432
Position (kb)
5838
5839
5840 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5837.137 kb on + strand, within BT4430 at 5837.232 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.312 kb on - strand, within BT4430 at 5837.354 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.463 kb on - strand, within BT4430 at 5837.492 kb on + strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.605 kb on + strand, within BT4430 at 5837.650 kb on - strand at 5837.666 kb on - strand at 5837.726 kb on - strand at 5837.851 kb on + strand at 5837.869 kb on + strand at 5837.913 kb on - strand at 5838.006 kb on - strand at 5838.109 kb on + strand at 5838.119 kb on + strand at 5838.175 kb on - strand at 5838.175 kb on - strand at 5838.246 kb on + strand, within BT4431 at 5838.248 kb on + strand, within BT4431 at 5838.324 kb on + strand, within BT4431 at 5838.374 kb on + strand, within BT4431 at 5838.375 kb on - strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.377 kb on - strand, within BT4431 at 5838.379 kb on - strand, within BT4431 at 5838.381 kb on - strand, within BT4431 at 5838.547 kb on - strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.720 kb on - strand, within BT4431 at 5838.745 kb on + strand, within BT4431 at 5838.763 kb on + strand, within BT4431 at 5838.853 kb on - strand, within BT4431 at 5838.971 kb on + strand, within BT4431 at 5838.972 kb on - strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.154 kb on - strand, within BT4431 at 5839.154 kb on - strand, within BT4431 at 5839.195 kb on - strand, within BT4431 at 5839.196 kb on - strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.269 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.494 kb on - strand, within BT4431 at 5839.497 kb on + strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.504 kb on - strand, within BT4431 at 5839.529 kb on - strand, within BT4431 at 5839.624 kb on - strand, within BT4431 at 5839.676 kb on - strand at 5839.693 kb on - strand at 5840.133 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.265 kb on - strand at 5840.276 kb on - strand at 5840.346 kb on - strand at 5840.426 kb on - strand at 5840.454 kb on - strand at 5840.509 kb on - strand at 5840.650 kb on - strand at 5840.680 kb on - strand at 5840.680 kb on - strand at 5840.683 kb on - strand at 5840.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,837,137 + BT4430 0.70 -0.3 5,837,232 - BT4430 0.74 +0.9 5,837,283 - BT4430 0.76 +0.4 5,837,283 - BT4430 0.76 -2.0 5,837,312 - BT4430 0.78 -0.9 5,837,354 + BT4430 0.79 +1.1 5,837,462 + BT4430 0.84 +1.4 5,837,462 + BT4430 0.84 +0.9 5,837,463 - BT4430 0.84 +1.2 5,837,492 + BT4430 0.85 -1.2 5,837,596 - BT4430 0.89 -1.3 5,837,596 - BT4430 0.89 -1.1 5,837,605 + BT4430 0.90 +1.4 5,837,650 - -0.4 5,837,666 - -0.4 5,837,726 - -1.0 5,837,851 + -1.3 5,837,869 + -1.2 5,837,913 - +0.7 5,838,006 - +0.4 5,838,109 + +0.8 5,838,119 + +0.3 5,838,175 - -1.1 5,838,175 - +0.1 5,838,246 + BT4431 0.11 -0.0 5,838,248 + BT4431 0.11 +1.8 5,838,324 + BT4431 0.15 -0.1 5,838,374 + BT4431 0.18 -3.1 5,838,375 - BT4431 0.18 +0.4 5,838,376 + BT4431 0.18 -1.4 5,838,376 + BT4431 0.18 -2.7 5,838,376 + BT4431 0.18 -3.3 5,838,377 - BT4431 0.18 -0.8 5,838,379 - BT4431 0.18 +0.6 5,838,381 - BT4431 0.18 +0.9 5,838,547 - BT4431 0.27 +1.4 5,838,635 + BT4431 0.32 -3.7 5,838,635 + BT4431 0.32 +1.4 5,838,720 - BT4431 0.37 +1.9 5,838,745 + BT4431 0.38 -2.4 5,838,763 + BT4431 0.39 -3.2 5,838,853 - BT4431 0.44 +0.5 5,838,971 + BT4431 0.51 -2.0 5,838,972 - BT4431 0.51 +4.2 5,839,015 + BT4431 0.53 -2.9 5,839,015 + BT4431 0.53 -0.6 5,839,016 - BT4431 0.53 -1.3 5,839,016 - BT4431 0.53 -2.5 5,839,116 - BT4431 0.59 -2.0 5,839,116 - BT4431 0.59 -1.7 5,839,154 - BT4431 0.61 -1.4 5,839,154 - BT4431 0.61 -1.6 5,839,195 - BT4431 0.63 +0.8 5,839,196 - BT4431 0.63 +0.4 5,839,209 + BT4431 0.64 -4.2 5,839,209 + BT4431 0.64 -2.6 5,839,269 + BT4431 0.67 -3.1 5,839,493 + BT4431 0.80 -2.3 5,839,493 + BT4431 0.80 -1.9 5,839,494 - BT4431 0.80 -1.0 5,839,497 + BT4431 0.80 -3.1 5,839,498 - BT4431 0.80 -1.7 5,839,498 - BT4431 0.80 +0.2 5,839,498 - BT4431 0.80 +0.7 5,839,504 - BT4431 0.81 +1.4 5,839,529 - BT4431 0.82 -2.3 5,839,624 - BT4431 0.87 -2.0 5,839,676 - -0.2 5,839,693 - -0.6 5,840,133 - BT4432 0.66 -1.7 5,840,160 - BT4432 0.73 -0.4 5,840,160 - BT4432 0.73 -0.4 5,840,222 - BT4432 0.88 -2.1 5,840,222 - BT4432 0.88 -0.6 5,840,222 - BT4432 0.88 -1.1 5,840,265 - +1.4 5,840,276 - +1.7 5,840,346 - +0.2 5,840,426 - -1.6 5,840,454 - +0.1 5,840,509 - -0.6 5,840,650 - +0.1 5,840,680 - -0.1 5,840,680 - -0.9 5,840,683 - +0.6 5,840,719 - -0.7
Or see this region's nucleotide sequence