Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4079 and BT4080 are separated by 184 nucleotides BT4080 and BT4081 are separated by 31 nucleotides
BT4079: BT4079 - hypothetical protein (NCBI ptt file), at 5,319,485 to 5,321,245
BT4079
BT4080: BT4080 - conserved hypothetical protein (NCBI ptt file), at 5,321,430 to 5,322,800
BT4080
BT4081: BT4081 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,322,832 to 5,325,933
BT4081
Position (kb)
5321
5322
5323 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5320.520 kb on - strand, within BT4079 at 5320.531 kb on + strand, within BT4079 at 5320.531 kb on + strand, within BT4079 at 5320.532 kb on - strand, within BT4079 at 5320.532 kb on - strand, within BT4079 at 5320.578 kb on - strand, within BT4079 at 5320.606 kb on - strand, within BT4079 at 5320.614 kb on - strand, within BT4079 at 5320.624 kb on - strand, within BT4079 at 5320.666 kb on - strand, within BT4079 at 5320.753 kb on - strand, within BT4079 at 5320.766 kb on - strand, within BT4079 at 5320.766 kb on - strand, within BT4079 at 5320.769 kb on - strand, within BT4079 at 5320.769 kb on - strand, within BT4079 at 5320.784 kb on + strand, within BT4079 at 5320.784 kb on + strand, within BT4079 at 5320.785 kb on - strand, within BT4079 at 5320.825 kb on - strand, within BT4079 at 5320.921 kb on - strand, within BT4079 at 5320.941 kb on + strand, within BT4079 at 5321.003 kb on - strand, within BT4079 at 5321.003 kb on - strand, within BT4079 at 5321.006 kb on + strand, within BT4079 at 5321.035 kb on + strand, within BT4079 at 5321.036 kb on - strand, within BT4079 at 5321.060 kb on - strand, within BT4079 at 5321.096 kb on - strand at 5321.175 kb on - strand at 5321.214 kb on + strand at 5321.215 kb on - strand at 5321.273 kb on - strand at 5321.315 kb on + strand at 5321.604 kb on - strand, within BT4080 at 5321.631 kb on - strand, within BT4080 at 5321.631 kb on - strand, within BT4080 at 5321.697 kb on - strand, within BT4080 at 5321.697 kb on - strand, within BT4080 at 5321.697 kb on - strand, within BT4080 at 5321.697 kb on - strand, within BT4080 at 5321.729 kb on + strand, within BT4080 at 5321.729 kb on + strand, within BT4080 at 5321.730 kb on - strand, within BT4080 at 5321.730 kb on - strand, within BT4080 at 5321.761 kb on + strand, within BT4080 at 5321.762 kb on - strand, within BT4080 at 5321.767 kb on + strand, within BT4080 at 5321.768 kb on - strand, within BT4080 at 5321.768 kb on - strand, within BT4080 at 5321.780 kb on - strand, within BT4080 at 5321.829 kb on - strand, within BT4080 at 5321.876 kb on + strand, within BT4080 at 5321.877 kb on - strand, within BT4080 at 5321.877 kb on - strand, within BT4080 at 5321.909 kb on + strand, within BT4080 at 5321.910 kb on - strand, within BT4080 at 5321.922 kb on - strand, within BT4080 at 5321.963 kb on + strand, within BT4080 at 5321.964 kb on - strand, within BT4080 at 5322.017 kb on - strand, within BT4080 at 5322.035 kb on + strand, within BT4080 at 5322.113 kb on + strand, within BT4080 at 5322.130 kb on - strand, within BT4080 at 5322.151 kb on - strand, within BT4080 at 5322.162 kb on - strand, within BT4080 at 5322.162 kb on - strand, within BT4080 at 5322.207 kb on - strand, within BT4080 at 5322.207 kb on - strand, within BT4080 at 5322.284 kb on - strand, within BT4080 at 5322.311 kb on - strand, within BT4080 at 5322.376 kb on - strand, within BT4080 at 5322.431 kb on + strand, within BT4080 at 5322.440 kb on + strand, within BT4080 at 5322.446 kb on + strand, within BT4080 at 5322.534 kb on - strand, within BT4080 at 5322.609 kb on - strand, within BT4080 at 5322.636 kb on + strand, within BT4080 at 5322.636 kb on + strand, within BT4080 at 5322.637 kb on - strand, within BT4080 at 5322.637 kb on - strand, within BT4080 at 5322.645 kb on - strand, within BT4080 at 5322.746 kb on - strand at 5322.799 kb on - strand at 5322.843 kb on - strand at 5322.885 kb on - strand at 5322.921 kb on + strand at 5322.966 kb on - strand at 5322.967 kb on + strand at 5322.973 kb on - strand at 5322.995 kb on + strand at 5322.996 kb on - strand at 5322.996 kb on - strand at 5323.003 kb on - strand at 5323.006 kb on - strand at 5323.039 kb on + strand at 5323.120 kb on - strand at 5323.161 kb on - strand, within BT4081 at 5323.173 kb on + strand, within BT4081 at 5323.248 kb on + strand, within BT4081 at 5323.250 kb on + strand, within BT4081 at 5323.255 kb on - strand, within BT4081 at 5323.262 kb on + strand, within BT4081 at 5323.263 kb on - strand, within BT4081 at 5323.363 kb on - strand, within BT4081 at 5323.366 kb on - strand, within BT4081 at 5323.366 kb on - strand, within BT4081 at 5323.499 kb on + strand, within BT4081 at 5323.503 kb on - strand, within BT4081 at 5323.503 kb on - strand, within BT4081 at 5323.561 kb on - strand at 5323.578 kb on - strand, within BT4081 at 5323.594 kb on + strand, within BT4081 at 5323.595 kb on - strand, within BT4081 at 5323.660 kb on - strand, within BT4081 at 5323.664 kb on - strand, within BT4081 at 5323.700 kb on + strand, within BT4081 at 5323.774 kb on + strand, within BT4081 at 5323.775 kb on - strand, within BT4081 at 5323.775 kb on - strand, within BT4081 at 5323.779 kb on - strand, within BT4081
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,320,520 - BT4079 0.59 -0.8 5,320,531 + BT4079 0.59 -0.8 5,320,531 + BT4079 0.59 +0.0 5,320,532 - BT4079 0.59 -0.4 5,320,532 - BT4079 0.59 +0.3 5,320,578 - BT4079 0.62 -0.1 5,320,606 - BT4079 0.64 +1.5 5,320,614 - BT4079 0.64 +0.8 5,320,624 - BT4079 0.65 -3.1 5,320,666 - BT4079 0.67 -2.0 5,320,753 - BT4079 0.72 -3.5 5,320,766 - BT4079 0.73 +0.5 5,320,766 - BT4079 0.73 -0.8 5,320,769 - BT4079 0.73 -1.0 5,320,769 - BT4079 0.73 +0.5 5,320,784 + BT4079 0.74 +1.4 5,320,784 + BT4079 0.74 +0.0 5,320,785 - BT4079 0.74 -1.8 5,320,825 - BT4079 0.76 -0.3 5,320,921 - BT4079 0.82 +0.5 5,320,941 + BT4079 0.83 -0.3 5,321,003 - BT4079 0.86 -0.2 5,321,003 - BT4079 0.86 -1.6 5,321,006 + BT4079 0.86 +0.1 5,321,035 + BT4079 0.88 -0.7 5,321,036 - BT4079 0.88 -1.5 5,321,060 - BT4079 0.89 -0.5 5,321,096 - -2.3 5,321,175 - -1.7 5,321,214 + +0.4 5,321,215 - -0.3 5,321,273 - -1.5 5,321,315 + -1.3 5,321,604 - BT4080 0.13 -0.5 5,321,631 - BT4080 0.15 -1.1 5,321,631 - BT4080 0.15 +0.6 5,321,697 - BT4080 0.19 +1.4 5,321,697 - BT4080 0.19 -0.2 5,321,697 - BT4080 0.19 +0.9 5,321,697 - BT4080 0.19 +1.0 5,321,729 + BT4080 0.22 +0.5 5,321,729 + BT4080 0.22 +0.3 5,321,730 - BT4080 0.22 +2.5 5,321,730 - BT4080 0.22 +1.5 5,321,761 + BT4080 0.24 +0.6 5,321,762 - BT4080 0.24 -1.1 5,321,767 + BT4080 0.25 +0.5 5,321,768 - BT4080 0.25 +0.0 5,321,768 - BT4080 0.25 -1.6 5,321,780 - BT4080 0.26 +0.2 5,321,829 - BT4080 0.29 -2.0 5,321,876 + BT4080 0.33 +2.6 5,321,877 - BT4080 0.33 -1.1 5,321,877 - BT4080 0.33 +0.2 5,321,909 + BT4080 0.35 -0.8 5,321,910 - BT4080 0.35 +1.5 5,321,922 - BT4080 0.36 +2.3 5,321,963 + BT4080 0.39 -0.9 5,321,964 - BT4080 0.39 -2.0 5,322,017 - BT4080 0.43 +0.5 5,322,035 + BT4080 0.44 -1.0 5,322,113 + BT4080 0.50 -0.2 5,322,130 - BT4080 0.51 -1.8 5,322,151 - BT4080 0.53 -0.5 5,322,162 - BT4080 0.53 +1.7 5,322,162 - BT4080 0.53 -0.1 5,322,207 - BT4080 0.57 +0.9 5,322,207 - BT4080 0.57 -1.5 5,322,284 - BT4080 0.62 +0.4 5,322,311 - BT4080 0.64 +1.6 5,322,376 - BT4080 0.69 -0.8 5,322,431 + BT4080 0.73 +1.1 5,322,440 + BT4080 0.74 +1.4 5,322,446 + BT4080 0.74 -0.1 5,322,534 - BT4080 0.81 +0.1 5,322,609 - BT4080 0.86 -2.2 5,322,636 + BT4080 0.88 -1.0 5,322,636 + BT4080 0.88 -0.5 5,322,637 - BT4080 0.88 +0.2 5,322,637 - BT4080 0.88 +1.1 5,322,645 - BT4080 0.89 +0.8 5,322,746 - -0.2 5,322,799 - +0.0 5,322,843 - -1.2 5,322,885 - -0.0 5,322,921 + +1.5 5,322,966 - -0.5 5,322,967 + -0.6 5,322,973 - -0.0 5,322,995 + -0.2 5,322,996 - -0.9 5,322,996 - +1.5 5,323,003 - +0.1 5,323,006 - +0.8 5,323,039 + +0.5 5,323,120 - -1.4 5,323,161 - BT4081 0.11 -0.7 5,323,173 + BT4081 0.11 +0.7 5,323,248 + BT4081 0.13 -1.3 5,323,250 + BT4081 0.13 -1.1 5,323,255 - BT4081 0.14 -1.1 5,323,262 + BT4081 0.14 +0.0 5,323,263 - BT4081 0.14 -1.0 5,323,363 - BT4081 0.17 +0.2 5,323,366 - BT4081 0.17 -0.5 5,323,366 - BT4081 0.17 +0.3 5,323,499 + BT4081 0.22 -0.4 5,323,503 - BT4081 0.22 -2.1 5,323,503 - BT4081 0.22 -1.3 5,323,561 - +0.5 5,323,578 - BT4081 0.24 -0.2 5,323,594 + BT4081 0.25 +0.5 5,323,595 - BT4081 0.25 -0.1 5,323,660 - BT4081 0.27 +0.7 5,323,664 - BT4081 0.27 +0.5 5,323,700 + BT4081 0.28 -1.2 5,323,774 + BT4081 0.30 +0.2 5,323,775 - BT4081 0.30 -0.5 5,323,775 - BT4081 0.30 -1.4 5,323,779 - BT4081 0.31 +0.2
Or see this region's nucleotide sequence