Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3914 and BT3915 are separated by 397 nucleotides BT3915 and BT3916 are separated by 142 nucleotides
BT3914: BT3914 - hypothetical protein (NCBI ptt file), at 5,088,276 to 5,089,178
BT3914
BT3915: BT3915 - hypothetical protein (NCBI ptt file), at 5,089,576 to 5,089,818
BT3915
BT3916: BT3916 - site-specific recombinase IntIA (NCBI ptt file), at 5,089,961 to 5,090,911
BT3916
Position (kb)
5089
5090 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5088.616 kb on - strand, within BT3914 at 5088.718 kb on - strand, within BT3914 at 5088.718 kb on - strand, within BT3914 at 5088.754 kb on - strand, within BT3914 at 5088.799 kb on + strand, within BT3914 at 5088.808 kb on + strand, within BT3914 at 5088.808 kb on + strand at 5088.809 kb on - strand, within BT3914 at 5088.814 kb on - strand, within BT3914 at 5088.815 kb on + strand, within BT3914 at 5088.816 kb on - strand, within BT3914 at 5088.837 kb on + strand, within BT3914 at 5088.838 kb on - strand, within BT3914 at 5088.838 kb on - strand, within BT3914 at 5088.893 kb on + strand, within BT3914 at 5088.894 kb on - strand, within BT3914 at 5088.917 kb on - strand, within BT3914 at 5088.934 kb on - strand, within BT3914 at 5088.938 kb on - strand, within BT3914 at 5088.965 kb on + strand, within BT3914 at 5088.970 kb on - strand, within BT3914 at 5088.970 kb on - strand, within BT3914 at 5089.025 kb on + strand, within BT3914 at 5089.172 kb on + strand at 5089.184 kb on + strand at 5089.184 kb on + strand at 5089.185 kb on - strand at 5089.228 kb on + strand at 5089.290 kb on - strand at 5089.291 kb on + strand at 5089.333 kb on - strand at 5089.477 kb on + strand at 5089.483 kb on + strand at 5089.483 kb on + strand at 5089.487 kb on + strand at 5089.635 kb on - strand, within BT3915 at 5089.638 kb on - strand, within BT3915 at 5089.757 kb on - strand, within BT3915 at 5089.813 kb on - strand at 5089.813 kb on - strand at 5089.817 kb on - strand at 5089.817 kb on - strand at 5089.837 kb on - strand at 5089.846 kb on - strand at 5089.934 kb on - strand at 5089.938 kb on - strand at 5090.044 kb on + strand at 5090.057 kb on + strand, within BT3916 at 5090.058 kb on - strand, within BT3916 at 5090.060 kb on - strand, within BT3916 at 5090.098 kb on + strand, within BT3916 at 5090.103 kb on - strand, within BT3916 at 5090.103 kb on - strand, within BT3916 at 5090.103 kb on - strand, within BT3916 at 5090.103 kb on - strand, within BT3916 at 5090.153 kb on + strand, within BT3916 at 5090.154 kb on - strand, within BT3916 at 5090.154 kb on - strand, within BT3916 at 5090.192 kb on - strand, within BT3916 at 5090.196 kb on - strand, within BT3916 at 5090.197 kb on + strand, within BT3916 at 5090.198 kb on - strand, within BT3916 at 5090.277 kb on + strand, within BT3916 at 5090.344 kb on + strand, within BT3916 at 5090.474 kb on + strand, within BT3916 at 5090.586 kb on - strand, within BT3916 at 5090.611 kb on + strand, within BT3916 at 5090.611 kb on + strand, within BT3916 at 5090.770 kb on + strand, within BT3916 at 5090.771 kb on - strand, within BT3916 at 5090.772 kb on + strand, within BT3916 at 5090.773 kb on - strand, within BT3916 at 5090.773 kb on - strand, within BT3916 at 5090.773 kb on - strand, within BT3916
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,088,616 - BT3914 0.38 +0.0 5,088,718 - BT3914 0.49 +1.2 5,088,718 - BT3914 0.49 -0.4 5,088,754 - BT3914 0.53 -0.6 5,088,799 + BT3914 0.58 +0.1 5,088,808 + BT3914 0.59 +0.2 5,088,808 + +1.6 5,088,809 - BT3914 0.59 +1.4 5,088,814 - BT3914 0.60 -2.5 5,088,815 + BT3914 0.60 +0.6 5,088,816 - BT3914 0.60 -0.3 5,088,837 + BT3914 0.62 -0.8 5,088,838 - BT3914 0.62 -0.5 5,088,838 - BT3914 0.62 +0.0 5,088,893 + BT3914 0.68 -0.1 5,088,894 - BT3914 0.68 +0.6 5,088,917 - BT3914 0.71 -1.5 5,088,934 - BT3914 0.73 +0.7 5,088,938 - BT3914 0.73 +0.7 5,088,965 + BT3914 0.76 -0.9 5,088,970 - BT3914 0.77 +0.8 5,088,970 - BT3914 0.77 -0.0 5,089,025 + BT3914 0.83 +1.6 5,089,172 + -2.2 5,089,184 + +0.2 5,089,184 + -1.1 5,089,185 - -0.4 5,089,228 + -0.2 5,089,290 - -1.1 5,089,291 + -1.5 5,089,333 - -0.2 5,089,477 + -2.6 5,089,483 + -0.0 5,089,483 + -0.0 5,089,487 + +1.8 5,089,635 - BT3915 0.24 -2.5 5,089,638 - BT3915 0.26 +1.6 5,089,757 - BT3915 0.74 -2.1 5,089,813 - -2.6 5,089,813 - -0.2 5,089,817 - -0.1 5,089,817 - -0.0 5,089,837 - -0.0 5,089,846 - -0.7 5,089,934 - +0.3 5,089,938 - -1.7 5,090,044 + +1.4 5,090,057 + BT3916 0.10 -2.1 5,090,058 - BT3916 0.10 -1.2 5,090,060 - BT3916 0.10 +0.4 5,090,098 + BT3916 0.14 -0.9 5,090,103 - BT3916 0.15 +0.5 5,090,103 - BT3916 0.15 -1.6 5,090,103 - BT3916 0.15 +0.2 5,090,103 - BT3916 0.15 +1.6 5,090,153 + BT3916 0.20 -2.1 5,090,154 - BT3916 0.20 +0.4 5,090,154 - BT3916 0.20 -0.8 5,090,192 - BT3916 0.24 +0.1 5,090,196 - BT3916 0.25 -0.0 5,090,197 + BT3916 0.25 -0.1 5,090,198 - BT3916 0.25 -1.7 5,090,277 + BT3916 0.33 +0.4 5,090,344 + BT3916 0.40 +1.6 5,090,474 + BT3916 0.54 -0.0 5,090,586 - BT3916 0.66 +0.6 5,090,611 + BT3916 0.68 -2.2 5,090,611 + BT3916 0.68 +2.6 5,090,770 + BT3916 0.85 -0.4 5,090,771 - BT3916 0.85 -1.5 5,090,772 + BT3916 0.85 -1.0 5,090,773 - BT3916 0.85 -0.7 5,090,773 - BT3916 0.85 -0.4 5,090,773 - BT3916 0.85 -1.4
Or see this region's nucleotide sequence