Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3268 and BT3269 are separated by 834 nucleotides BT3269 and BT3270 are separated by 515 nucleotides
BT3268: BT3268 - glucose-inhibited division protein A (NCBI ptt file), at 4,171,817 to 4,173,703
BT3268
BT3269: BT3269 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 4,174,538 to 4,175,110
BT3269
BT3270: BT3270 - putative anti-sigma factor (NCBI ptt file), at 4,175,626 to 4,176,834
BT3270
Position (kb)
4174
4175
4176 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4173.684 kb on - strand at 4173.684 kb on - strand at 4173.712 kb on - strand at 4173.712 kb on - strand at 4173.712 kb on - strand at 4173.912 kb on - strand at 4173.933 kb on + strand at 4173.933 kb on + strand at 4173.934 kb on - strand at 4174.036 kb on - strand at 4174.140 kb on + strand at 4174.140 kb on + strand at 4174.169 kb on - strand at 4174.185 kb on - strand at 4174.196 kb on + strand at 4174.196 kb on + strand at 4174.197 kb on - strand at 4174.211 kb on - strand at 4174.235 kb on - strand at 4174.238 kb on - strand at 4174.238 kb on - strand at 4174.244 kb on + strand at 4174.244 kb on + strand at 4174.251 kb on - strand at 4174.360 kb on - strand at 4174.390 kb on - strand at 4174.395 kb on - strand at 4174.417 kb on + strand at 4174.434 kb on + strand at 4174.435 kb on - strand at 4174.435 kb on - strand at 4174.454 kb on - strand at 4174.456 kb on - strand at 4174.468 kb on + strand at 4174.513 kb on - strand at 4174.523 kb on + strand at 4174.616 kb on + strand, within BT3269 at 4174.617 kb on - strand, within BT3269 at 4174.617 kb on - strand, within BT3269 at 4174.696 kb on + strand, within BT3269 at 4174.697 kb on - strand, within BT3269 at 4174.698 kb on + strand, within BT3269 at 4174.698 kb on + strand, within BT3269 at 4174.714 kb on + strand, within BT3269 at 4174.715 kb on - strand, within BT3269 at 4174.751 kb on + strand, within BT3269 at 4174.770 kb on - strand, within BT3269 at 4174.787 kb on + strand, within BT3269 at 4174.788 kb on - strand, within BT3269 at 4174.819 kb on - strand, within BT3269 at 4174.868 kb on + strand, within BT3269 at 4174.908 kb on - strand, within BT3269 at 4174.908 kb on - strand, within BT3269 at 4174.914 kb on - strand, within BT3269 at 4174.914 kb on - strand, within BT3269 at 4174.915 kb on + strand, within BT3269 at 4175.015 kb on - strand, within BT3269 at 4175.015 kb on - strand, within BT3269 at 4175.044 kb on + strand, within BT3269 at 4175.078 kb on + strand at 4175.199 kb on - strand at 4175.225 kb on + strand at 4175.226 kb on - strand at 4175.231 kb on - strand at 4175.231 kb on - strand at 4175.233 kb on - strand at 4175.233 kb on - strand at 4175.233 kb on - strand at 4175.281 kb on + strand at 4175.282 kb on - strand at 4175.390 kb on - strand at 4175.398 kb on + strand at 4175.398 kb on + strand at 4175.410 kb on - strand at 4175.571 kb on + strand at 4175.572 kb on - strand at 4175.582 kb on - strand at 4175.615 kb on + strand at 4175.727 kb on - strand at 4175.730 kb on + strand at 4175.797 kb on - strand, within BT3270 at 4175.909 kb on - strand, within BT3270 at 4175.954 kb on - strand, within BT3270 at 4176.086 kb on + strand, within BT3270 at 4176.092 kb on + strand, within BT3270
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 4,173,684 - -1.2 4,173,684 - -0.2 4,173,712 - +2.0 4,173,712 - +0.4 4,173,712 - -1.4 4,173,912 - -0.3 4,173,933 + +0.2 4,173,933 + -0.4 4,173,934 - +0.8 4,174,036 - -0.1 4,174,140 + -0.5 4,174,140 + -1.8 4,174,169 - +0.4 4,174,185 - +0.8 4,174,196 + -1.9 4,174,196 + -0.4 4,174,197 - -1.0 4,174,211 - +0.1 4,174,235 - +2.8 4,174,238 - +0.5 4,174,238 - +0.8 4,174,244 + -1.8 4,174,244 + +0.9 4,174,251 - -1.8 4,174,360 - +0.1 4,174,390 - -1.8 4,174,395 - -0.6 4,174,417 + -0.3 4,174,434 + -0.1 4,174,435 - +0.4 4,174,435 - -1.7 4,174,454 - -2.0 4,174,456 - -0.1 4,174,468 + -3.2 4,174,513 - -1.0 4,174,523 + -2.5 4,174,616 + BT3269 0.14 -1.8 4,174,617 - BT3269 0.14 -1.8 4,174,617 - BT3269 0.14 +0.9 4,174,696 + BT3269 0.28 -1.7 4,174,697 - BT3269 0.28 -0.3 4,174,698 + BT3269 0.28 -0.5 4,174,698 + BT3269 0.28 +0.8 4,174,714 + BT3269 0.31 -1.3 4,174,715 - BT3269 0.31 -1.9 4,174,751 + BT3269 0.37 +1.8 4,174,770 - BT3269 0.40 +1.8 4,174,787 + BT3269 0.43 -4.6 4,174,788 - BT3269 0.44 +0.8 4,174,819 - BT3269 0.49 -1.7 4,174,868 + BT3269 0.58 -2.1 4,174,908 - BT3269 0.65 -1.0 4,174,908 - BT3269 0.65 +0.2 4,174,914 - BT3269 0.66 +0.2 4,174,914 - BT3269 0.66 -0.4 4,174,915 + BT3269 0.66 -1.3 4,175,015 - BT3269 0.83 -1.4 4,175,015 - BT3269 0.83 -2.1 4,175,044 + BT3269 0.88 -2.0 4,175,078 + -2.7 4,175,199 - -0.2 4,175,225 + -1.9 4,175,226 - +0.5 4,175,231 - +1.8 4,175,231 - +0.3 4,175,233 - +0.7 4,175,233 - -0.5 4,175,233 - +0.7 4,175,281 + -2.2 4,175,282 - +0.4 4,175,390 - -1.0 4,175,398 + +0.5 4,175,398 + -1.7 4,175,410 - +1.8 4,175,571 + +1.8 4,175,572 - -0.4 4,175,582 - -3.0 4,175,615 + -2.1 4,175,727 - +0.2 4,175,730 + -1.4 4,175,797 - BT3270 0.14 -0.2 4,175,909 - BT3270 0.23 -3.4 4,175,954 - BT3270 0.27 -1.3 4,176,086 + BT3270 0.38 -1.5 4,176,092 + BT3270 0.39 -3.0
Or see this region's nucleotide sequence